Studies on regulation of matrix ion dynamics and energy metabolism by mitochondria NCX

2010 Fiscal Year Final Research Report

• Project/Area Number: 20390057
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General physiology
• Research Institution: Kyoto University
• Principal Investigator: MATSUOKA Satoshi Kyoto University, 医学研究科, 特定准教授 (00263096)
• Co-Investigator(Kenkyū-buntansha): KIM Bongju 京都大学, 医学研究科, 研究員 (80511823)
• Co-Investigator(Renkei-kenkyūsha): TAKATSUKA Kenji 京都大学, 医学研究科, 研究員 (70378701) ; TAKEUCHI Ayako 京都大学, 医学研究科, 助教 (00378704)
• Project Period (FY): 2008 – 2010
• Keywords: ミトコンドリア / シミュレーション / Na / Ca交換 / カルシウム

Research Abstract

The aim of this study was quantitative elucidation of physiological function of mitochondria. Mitochondrial and cytoplasmic Ca dynamics were studied by using Ca-sensitive fluorescent dyes in cardiac myocytes. Based on these data and previously published data, a mathematical model of mitochondria which can well reproduce mitochondrial Ca, Na and H changes was newly developed. A comprehensive mitochondria model was accomplished by integrating the model with the metabolic model including oxidative phosphorylation and TCA cycle. It was suggested that Ca- and phosphate-dependent regulatory processes are important in keeping the balance of energy metabolites.

Research Products

• [Journal Article] Induction and enhancement of cardiac cell differentiation from mouse and human induced pluripotent stem cells with cyclosporin-a. (2010)
  • Author(s): Fujiwara M, Yan P, Otsuji TG, Narazaki G, Uosaki H, Fukushima H, Kuwahara K, Harada M, Matsuda H, Matsuoka S, Okita K, Takahashi K, Nakagawa M, Ikeda T, Sakata R, Mummery CL, Nakatsuji N, Yamanaka S, Nakao K, Yamashita JK.
  • Journal Title: PLoS One. Vol6, No2 Pages: 16734
  • Peer Reviewed
• [Journal Article] Magnesium gating of cardiac gap junction channels. (2010)
  • Author(s): Matsuda H, Kurata Y, Oka C, Matsuoka S, Noma A.
  • Journal Title: Progress in Biophysics and Molecular Biology. Vol103, No1 Pages: 102-110
  • Peer Reviewed
• [Journal Article] Controlled release of matrix metalloproteinase-1 plasmid DNA prevents left ventricular remodeling in chronic myocardial infarction of rats. (2009)
  • Author(s): Lin X, Jo H, Ishii TM, Fujita M, Fu M, Tambara K, Yamamoto M, Tabata Y, Komeda M, Matsuoka S.
  • Journal Title: Circulation Journal. Vol73, No12 Pages: 2315-2321
  • Peer Reviewed
• [Journal Article] Role of Mg^<2+> block of the inward rectifier K^+ current in cardiac repolarization reserve : a quantitative simulation. (2009)
  • Author(s): Ishihara K, Sarai N, Asakura K, Noma A, Matsuoka S.
  • Journal Title: Journal of molecular and cellular cardiology. Vol47, No1 Pages: 76-84
  • Peer Reviewed
• [Journal Article] Chronic partial unloading restores beta-adrenergic responsiveness and reverses receptor downregulation in failing rat hearts. (2009)
  • Author(s): Wang J, Tsukashita M, Nishina T, Marui A, Yoshikawa E, Muranaka H, Matsuoka S, Ikeda T.
  • Journal Title: Journal of Thoracic and Cardiovascular Surgery. Vol137, No2 Pages: 465-470
  • Peer Reviewed
• [Journal Article] Increased O_2 consumption in excitation-contraction coupling in hypertrophied rat heart slices related to increased Na^+-Ca^<2+>exchange activity. (2009)
  • Author(s): Shimizu J, Yamashita D, Misawa H, Tohne K, Matsuoka S, Kim B, Takeuchi A, Nakajima-Takenaka C, Takaki M.
  • Journal Title: Journal of Physiological Sciences. Vol59, No1 Pages: 63-74
  • Peer Reviewed
• [Journal Article] Cyclosporin-A potently induces highly cardiogenic progenitors from embryonic stem cells. (2009)
  • Author(s): Yan P, Nagasawa A, Uosaki H, Sugimoto A, Yamamizu K, Teranishi M, Matsuda H, Matsuoka S, Ikeda T, Komeda M, Sakata R, Yamashita JK.
  • Journal Title: Biochemical and biophysical research communications. Vol379, No1 Pages: 115-120
  • Peer Reviewed
• [Journal Article] A Simulation study on the activation of cardiac CaMKII delta-isoform and its regulation by phosphatases. (2008)
  • Author(s): Chiba H, Schneider NS, Matsuoka S, Noma A.
  • Journal Title: Biophysical Journal. Vol95, No5 Pages: 2139-2149
  • Peer Reviewed
• [Journal Article] Directed and systematic differentiation of cardiovascular cells from mouse induced pluripotent stem cells. (2008)
  • Author(s): Narazaki G, Uosaki H, Teranishi M, Okita K, Kim B, Matsuoka S, Yamanaka S, Yamashita JK.
  • Journal Title: Circulation. Vol118, No5 Pages: 498-506
  • Peer Reviewed
• [Presentation] Mathematical modeling Ca2+ dependent regulation of cardiac mitochondria. International Symposium (2011)
  • Author(s): Satoshi Matsuoka.
  • Organizer: Computational Physiology of Cardiac Myocytes and Pancreatic β-cells ; Interaction between Energy Metabolism and Membrane Excitability.
  • Place of Presentation: 京都市
  • Year and Date: 2011-01-30
• [Presentation] コンピュータシミュレーションによる心臓機能の探索 (2010)
  • Author(s): 松岡達
  • Organizer: 第27回日本心電学会学術集会
  • Place of Presentation: 大分市
  • Year and Date: 2010-10-09
• [Presentation] A simulation study of compensated heart failure. BBSRC UK-Japan Collaboration (2010)
  • Author(s): Satoshi Matsuoka.
  • Organizer: Cardiac Electro-Mechanical Function : Cell-Organ Cross-Talk Revealed via Integration of experiments and Models.
  • Place of Presentation: 英国オックスフォード
  • Year and Date: 2010-09-07
• [Presentation] Role of Ca^<2+>-dependent regulation in mitochondrial energy metabolism. BBSRC UK-Japan Collaboration (2010)
  • Author(s): Ayako Takeuchi.
  • Organizer: Cardiac Electro-Mechanical Function : Cell-Organ Cross-Talk Revealed via Integration of experiments and Models.
  • Place of Presentation: 英国オックスフォード
  • Year and Date: 2010-09-06
• [Presentation] Simulation Study of Physiology and Pathology of Heart : An Integrated Cardiac Cell Model. (2010)
  • Author(s): Satoshi Matsuoka.
  • Organizer: 第74回日本循環器学会総会・学術集会
  • Place of Presentation: 京都市
  • Year and Date: 2010-03-05
• [Presentation] Model and Experimental studies on regulation of mitochondrial Ca by mitochondrial Na^+/Ca^<2+> exchanger. (2009)
  • Author(s): Satoshi Matsuoka.
  • Organizer: 36th International Congress of Physiological Science Satellite Symposium.
  • Place of Presentation: 香川県直島
  • Year and Date: 2009-08-03
• [Presentation] Role of mitochondrial Na^+/Ca^<2+> exchange on B cell receptor-mediated Ca2+ responses in DT40 cell. (2009)
  • Author(s): Bongju Kim, Satoshi Matsuoka
  • Organizer: 36th International Congress of Physiological Sciences.
  • Place of Presentation: 京都市
  • Year and Date: 2009-07-28
• [Presentation] 心筋細胞におけるエネルギー代謝のシミュレーション (2009)
  • Author(s): 松岡達
  • Organizer: 第26回日本心電学会学術集会
  • Place of Presentation: 京都市
  • Year and Date: 2009-07-04
• [Presentation] Computational integration of cardiac cell function. (2009)
  • Author(s): Satoshi Matsuoka.
  • Organizer: 2009 IUPS International Conference of Physiological Sciences.
  • Place of Presentation: 韓国Busan
  • Year and Date: 2009-01-16
• [Presentation] 心筋細胞機能の包括的シミュレーション (2008)
  • Author(s): 松岡達, Kyoto Model
  • Organizer: 自然科学研究機構「自然科学における階層と全体」平成20年度シンポジウム
  • Place of Presentation: 蒲郡市
  • Year and Date: 2008-12-17
• [Presentation] Computational integration of cardiac cell function. (2008)
  • Author(s): Satoshi Matsuoka.
  • Organizer: 2008 Annual Conference of the Korean Association of Biological Sciences.
  • Place of Presentation: 韓国Mokpo
  • Year and Date: 2008-08-20
• [Presentation] Cell/Biodynamics simulation project in Kyoto university : Medical systems biology of the heart. International Physiome Symposium2008 (2008)
  • Author(s): Satoshi Matsuoka.
  • Organizer: Toward Holistic Medicine : Physiome and Oriental Medicine.
  • Place of Presentation: 韓国Seoul
  • Year and Date: 2008-04-11