Analyses of herpesvirus-infected cells by real time imaging

2010 Fiscal Year Final Research Report

• Project/Area Number: 20390130
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: The University of Tokyo
• Principal Investigator: KAWAGUCHI Yasushi 東京大学, 医科学研究所, 准教授 (60292984)
• Co-Investigator(Kenkyū-buntansha): UEMA Masashi 東京大学, 医科学研究所, 特任研究員 (70456193)
• Project Period (FY): 2008 – 2010
• Keywords: HSV / リアルタイムイメージング

Research Abstract

Using the real-time imaging system, we identified the site of HSV-1 final envelopment as trans-Golgi network. Moreover, we identified a binding partner of UL12, which is involved in nuclear egress pathway, as nucleolin and showed that nucleolin was required for efficient nuclear egress of HSV-1 capsids.

Research Products

• [Journal Article] Non-muscle myosin IIA is a functional entry receptor for herpes simplex virus 1. (2010)
  • Author(s): J.Arii, H.Goto, T.Suenaga, M.Oyama, H.Kozuka-Hata, T.Imai, A.Minowa, H.Akashi, H.Arase, Y.Kawaoka, Y.Kawaguchi.
  • Journal Title: Nature 467 Pages: 859-862
• [Journal Article] A Single Amino Acid Substitution in Herpes simplex virus 1 Envelope Glycoprotein B at a Site Required for Binding to the Paired Immunoglobulin-like Type 2 Receptor a (PILRτ) Abrogates PILRτ-dependent Viral Entry and Reduces Pathogenesis. (2010)
  • Author(s): J.Arii, J.Wang, T.Morimoto, T.Suenaga, H.Akashi, H.Arase, Y.Kawaguchi.
  • Journal Title: J.Virol. 84 Pages: 10773-10783
• [Journal Article] Nucleolin is required for efficient nuclear egress of herpes simplex virus 1 nucleocapsids. (2010)
  • Author(s): K.Sagou, M.Uema, Y.Kawaguchi.
  • Journal Title: J.Virol. 84 Pages: 2110-2121
• [Journal Article] Effects of Phosphorylation of Herpes Simplex Virus 1 Envelope Glycoprotein B by Us3 Kinase In Vivo and In Vitro. (2010)
  • Author(s): T.Imai, K.Sagou, J.Arii, Y.Kawaguchi.
  • Journal Title: J.Virol. 84 Pages: 153-162
• [Journal Article] Selective control of type I IFN induction by the Rac activator DOCK2 during TLR!Vmediated plasmacytoid dendritic cell activation. (2010)
  • Author(s): K.Gotoh, Y.Tanaka, A.Nishikimi, R.Nakamura, H.Yamada, N.Maeda, T.Ishikawa, K.Hoshino, T.Uruno, Q.Cao, S.Higashi, Y.Kawaguchi, M.Enjoji, R.Takayanagi, T.Kaisho, Y.Yoshikai, Y.Fukui.
  • Journal Title: J.Exp.Med. 207 Pages: 721-730
• [Journal Article] Myelin-associated glycoprotein mediates membrane fusion and entry of neurotropic herpesviruses. (2010)
  • Author(s): T.Suenaga, T.Satoh, P.Somboonthum, Y.Kawaguchi, Y.Mori, H.Arase
  • Journal Title: Proc.Natl.Acad.Sci.USA 107 Pages: 866-871
• [Journal Article] Characterization of a thymidine kinase-deficient mutant of equine herpesvirus 4 and in vitro susceptibility of the virus to antiviral agents. (2010)
  • Author(s): W.Azab, K.Tsujimura, K.Kato, J.Arii, T.Morimoto, Y.Kawaguchi, Y.Tohya, T.Matsumura, H.Akashi.
  • Journal Title: Antiviral Res. 85 Pages: 389-395
• [Journal Article] Binding of herpes simplex virus glycoprotein B (gB) to PILR￡\ depends on specific sialylated O-linked glycans on gB. (2009)
  • Author(s): J.Wang, F.Fan, T.Satoh, J.Arii, L.L.Lanier, P.G.Spear, Y.Kawaguchi, H.Arase.
  • Journal Title: J.Virol. 83 Pages: 13042-13045
• [Journal Article] Differences in the Regulatory and Functional Effects of the Us3 Protein Kinase Activities of Herpes Simplex Virus 1 and 2. (2009)
  • Author(s): T.Morimoto, J.Arii, M.Tanaka, T.Sata, H.Akashi, M.Yamada, Y.Nishiyama, M.Uema, Y.Kawaguchi.
  • Journal Title: J.Virol. 83 Pages: 11624-11634
• [Journal Article] Herpesvirus protein ICP27 switches PML isoform by altering mRNA splicing. (2009)
  • Author(s): T.Nojima, T.Oshiro-Ideue, H.Nakanoya, H.Kawamura, T.Morimoto, Y.Kawaguchi, N.Kataoka,, M.Hagiwara.
  • Journal Title: Nucleic Acids Res. 37 Pages: 6515-6527
• [Journal Article] Analysis of herpesvirus host specificity determinants using herpesvirus genomes as bacterial artificial chromosomes. (2009)
  • Author(s): J.Arii, K.Kato, Y.Kawaguchi, Y.Tohya, H.Akashi.
  • Journal Title: Microbiol.Immunol. 53 Pages: 433-441
• [Journal Article] TRAF6 establishes innate immune responses by activating NF-kB and IRF7 upon sensing cytosolic viral RNA and DNA. (2009)
  • Author(s): H.Konno, T.Yamamoto, K.Yamazaki, J.Gohda, T.Akiyama, K.Semba, H.Goto, A.Kato, T.Yujiri, T.Imai, Y.Kawaguchi, B.Su, O.Takeuchi, S.Akira, Y.Tsunetsugu-Yokota, J.Inoue.
  • Journal Title: PLoS ONE 4 Pages: e5674
• [Journal Article] Epstein-Barr virus protein kinase BGLF4 interacts with viral transactivator BZLF1 and regulates its transactivation activity. (2009)
  • Author(s): R.Asai, A.Kato, Y.Kawaguchi.
  • Journal Title: J.Gen.Virol. 90 Pages: 1575-1581
• [Journal Article] Regulation of the Catalytic Activity of Herpes Simplex Virus 1 Protein Kinase Us3 by Auto-Phosphorylation and Its Role in Pathogenesis. (2009)
  • Author(s): K.Sagou, T.Imai, H.Sagara, M.Uema, Y.Kawaguchi.
  • Journal Title: J.Virol. 83 Pages: 5773-5783
• [Journal Article] Entry of herpes simplex virus 1 and other alphaherpesviruses via the paired immunoglobulin-like type 2 receptorハτ. (2009)
  • Author(s): J.Arii, M.Uema, T.Morimoto, H.Sagara, H.Akashi, E.Ono, H.Arase, Y.Kawaguchi.
  • Journal Title: J.Virol. 83 Pages: 4520-4527
• [Journal Article] Herpesvirus gB-induced fusion between the virion envelope and outer nuclear membrane during virus egress is regulated by the viral US3 kinase. (2009)
  • Author(s): T.W.Wisner, C.C.Wright, A.Kato, Y.Kawaguchi, F.Mou, J.D.Baines, R.J.Roller, D.C.Johnson.
  • Journal Title: J.Virol. 83 Pages: 3115-3126
• [Journal Article] Identification of multiple sites in herpes simplex virus genomes suitable for insertion of foreign genes. (2009)
  • Author(s): T.Morimoto, J.Arii, H.Akashi, Y.Kawaguchi.
  • Journal Title: Microbiol.Immunol. 53 Pages: 155-161
• [Journal Article] Herpes simplex virus 1 protein kinase Us3 phosphorylates viral envelope glycoprotein B and regulates its expression on the cell surface. (2009)
  • Author(s): A.Kato, J.Arii, I.Shiratori, H.Akashi, H.Arase, Y.Kawaguchi.
  • Journal Title: J.Virol. 83 Pages: 250-261
• [Journal Article] Identification of a physiological phosphorylation site of the Herpes simplex virus 1-encoded protein kinase Us3 which regulates its optimal catalytic activity in vitro and influences its function in infected cells. (2008)
  • Author(s): A.Kato, M.Tanaka, M.Yamamoto, R.Asai, T.Sata, Y.Nishiyama, Y.Kawaguchi.
  • Journal Title: J.Virol. 82 Pages: 6172-6189
• [Journal Article] Simultaneous tracking of capsid, tegument, and envelop protein localization in living cells infected with thriple fluorescent herpes simplex virus 1. (2008)
  • Author(s): K.Sugimoto, M.Uema, H.Sagara, M.Tanaka, T.Sata, Y.Hashimoto, Y.Kawaguchi.
  • Journal Title: J.Virol. 82 Pages: 5198-5211
• [Journal Article] PILRa is a herpes simplex-1 entry co-receptor that associates with glycoprotein B. (2008)
  • Author(s): T.Satoh, J.Arii, T.Suemaga, J.Wang, A.Kogure, J.Uehori, N.Arase, I.Shiratori, S.Tanaka, T.Satoh, J.Arii, T.Suemaga, J.Wang, A.Kogure, J.Uehori, N.Arase, I.Shiratori, S.Tanaka, Y.Kawaguchi, P.G.Spear, L.L.Lanier, H.Arase.
  • Journal Title: Cell 132 Pages: 1-10
• [Journal Article] Microarray analysis of transcriptional responses to infection by herpes simplex virus types 1 and 2 and their US3-deficient mutants. (2008)
  • Author(s): Kamakura, M., Nawa, A., Ushijima, Y., Goshima, F., Kawaguchi, Y., Kikkawa, F., Nishiyama, Y.
  • Journal Title: Microbes and Infection 10 Pages: 405-413
• [Journal Article] Evaluation of therapeutic effects of antiviral agents using a silkworm baculovirus infection model. (2008)
  • Author(s): Y.Orihara, H.Hamamoto, H.Kasuga, T.Shimada, Y.Kawaguchi, K.Shekimizu.
  • Journal Title: J.Gen.Virol. 89 Pages: 188-194
• [Journal Article] Primary target cells of herpes simplex virus type 1 in the hippocampus. (2008)
  • Author(s): Y.Ando, H.Kitamura, Y.Kawaguchi, Y.Koyanagi.
  • Journal Title: Microbes and Infection 10 Pages: 1514-1523
• [Journal Article] The Product of UL7 gene of Herpes simplex virus type 1 interacts with a Mitochondria protein, Adenine nucleotide translocator 2. (2008)
  • Author(s): M.Tanaka, T.Sata, Y.Kawaguchi.
  • Journal Title: Virology J. 5 Pages: 125-137
• [Journal Article] Role of the Herpes Simplex Virus 1 Us3 Kinase Phosphorylation Site and Endocytosis Motifs in EnvelopeGlycoprotein B in Its Intracellular Transport and Neurovirulence.
  • Author(s): T.Imai, J.Arii, A.Minowa, A.Kakimoto, N.Koyanagi, A.Kato, Y.Kawaguchi.
  • Journal Title: J.Virol. in press
• [Journal Article] Anterograde transport of herpes simplex virus capsids in neurons by Separate and Married mechanisms.
  • Author(s): T.W.Wisner, K.Sugimoto, P.Howard, Y.Kawaguchi, D.C.Johnson.
  • Journal Title: J.Virol. in press
• [Presentation] ヘルペスウイルスの新規受容体の同定 (2010)
  • Author(s): 川口寧
  • Organizer: 平成22年度筆界同大学遺伝子病制御研究所共同研究集会「感染・炎症・発癌」
  • Place of Presentation: 札幌(招待講演)
  • Year and Date: 20101200
• [Presentation] Non-muscle myosin IIA is a functional entry receptor for herpes simplex virus 1. (2010)
  • Author(s): 川口寧
  • Organizer: The 7th China-Japan Joint Laboratory Workshop-Pathogenesis, Gene Regulation and Signal Transduction
  • Place of Presentation: Beijing, China.
  • Year and Date: 20101000
• [Presentation] Regulation and functions of HSV Us3 protein kinases. (2010)
  • Author(s): 川口寧
  • Organizer: 14^<th> International Conference on Immunology and Prophylaxis of Human Herpesvirus Infections.
  • Place of Presentation: Kobe, Japan(招待講演)
  • Year and Date: 20101000
• [Presentation] Role of Us3 phosphorylation on HSV-1 gB in its endocytosis and pathogenesis. (2010)
  • Author(s): T.Imai, J.Arii, A.Kato, K.Sagou, Y.Kawaguchi
  • Organizer: 35^<th> International Herpesvirus Workshop
  • Place of Presentation: Salt Lake City, USA.
  • Year and Date: 20100700
• [Presentation] たった1アミノ酸のリン酸化によるウイルス病態発現の分子機構 (2010)
  • Author(s): 川口寧
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 札幌(招待講演)
  • Year and Date: 20100600
• [Presentation] Regulatory and functional effects of protein kinases encoded by herpesviruses on host cells. (2010)
  • Author(s): 川口寧
  • Organizer: Seminar in Reprogramming of host cells by microbial effectors in Institut fur Klinische und Molekulare Virologie Universitat Erlangen-Nurnberg Erlangen
  • Place of Presentation: Germany(招待講演)
  • Year and Date: 20100200
• [Presentation] ウイルスプロテインキナーゼの機能と病態への関与 (2010)
  • Author(s): 川口寧
  • Organizer: 平成22年度筆界同大学遺伝子病制御研究所共同研究集会「感染・炎症・発癌」
  • Place of Presentation: 札幌(招待講演)
  • Year and Date: 20100100
• [Presentation] Regulation of the catalytic activity of HSV protein kinase Us3 and its role in pathogenesis. (2009)
  • Author(s): K.Sagou, T.Morimoto, J.Arii, T.Imai, M.Tanaka, Y.Nishiyama, M.Uema, Y.Kawaguchi
  • Organizer: 34^<th> International Herpesvirus Workshop Ithaca
  • Place of Presentation: USA.
  • Year and Date: 20090700
• [Presentation] Entry of herpes simplex virus 1 and other aplhaherpesviruses via the paired immunoglobulin-like type 2 receptor . (2009)
  • Author(s): J.Arii, J.Wang, M.Uema, T.Morimoto, H.Sagara, H.Akashi, E.Ono, H.Arase, Y.Kawaguchi
  • Organizer: 34^<th> International Herpesvirus Workshop Ithaca
  • Place of Presentation: USA.
  • Year and Date: 20090700
• [Presentation] Regulation of the catalytic activity of HSVUs3 protein kinases in infected cells. (2009)
  • Author(s): 川口寧
  • Organizer: A Scientific Symposium for Dr.Bernard Roizman: Honoring six decades of scientific research, teaching and service.
  • Place of Presentation: Chicago, USA.
  • Year and Date: 20090500
• [Presentation] Molecular mechanisms of viral replication and pathogenesis unveiled by fluorescent herpes simplex viruses. (2008)
  • Author(s): 川口寧
  • Organizer: 8^<th> Awaji International Forum on Infection and Immunity.
  • Place of Presentation: Awaji, Japan(招待講演)
  • Year and Date: 20080900
• [Presentation] Herpesvirus vectors-How to generate the vectors.Educational Semminar (2008)
  • Author(s): 川口寧
  • Organizer: 第14回日本遺伝子治療学会
  • Place of Presentation: 札幌(招待講演)
  • Year and Date: 20080600
• [Presentation] ヘルペスウイルス生活環の可視化.そのインパクトと臨床応用 シンポジウム (Bench to Bed side) (2008)
  • Author(s): 川口寧
  • Organizer: 第49回日本臨床ウイルス学会
  • Place of Presentation: 犬山(招待講演)
  • Year and Date: 20080600
• [Remarks] ホームページ等
  • URL: http://www.ims.u-tokyo.ac.jp/Kawaguchi-lab/KawaguchiLabTop.html
• [Patent(Industrial Property Rights)] 組換えウイルス、これを保持する大腸菌およびその製造法 (2008)
  • Inventor(s): 川口寧、森本智美
  • Industrial Property Rights Holder: 国立大学法人東京大学
  • Industrial Property Number: 特願2008-142662
  • Filing Date: 2008-05-30