2010 Fiscal Year Final Research Report
Analysis of in vivo functions of LMIR3/4/5
| Project/Area Number |
20390279
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
膠原病・アレルギー・感染症内科学
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KITAURA Jiro The University of Tokyo, 医科学研究所, 助教 (30282651)
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| Co-Investigator(Kenkyū-buntansha) |
KITAMURA Toshio 東京大学, 医科学研究所, 教授 (20282527)
NAKAO Kazuki 理化学研究所, 動物実験ユニット, ユニットリーダー (20217657)
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| Project Period (FY) |
2008 – 2010
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| Keywords | ノックアウトマウス / ペア型免疫レセプター / ミエロイド / 自己免疫 / 自然免疫 |
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| Research Abstract |
In the present study, we have clarified the physiological functions of LMIR3/4/5. Co-engagement of FcεRI and LMIR3 impaired cytokine production in bone marrow-derived mast cells (BMMCs) induced by FcεRI crosslinking alone, demonstrating that LMIR3 is an inhibitory receptor. On the other hand, LMIR3 has a unique receptor to associate with FcRγ and thereby functions as an activating receptor in concert with TLR4 stimulation. We have identified T cell Ig mucin I (TIM1) by retrovirus-mediated expression cloning. Notably, LMIR5 deficiency suppressed TIM1-Fc-induced recruitment of neutrophils in the dorsal air pouch, and LMIR5 deficiency attenuated neutrophil accumulation in a model of ischemia/reperfusion injury in the kidneys in which TIM1 expression is up-regulated. In that model, LMIR5 deficiency resulted in ameliorated tubular necrosis and cast formation in the acute phase. Collectively, TIM1-LMIR5 interaction plays a physiological role in immune regulation by myeloid cells.
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Research Products
(33 results)
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[Journal Article] Two types of C/EBP. mutations play distinct but collaborative roles in leukemogenesis : Lessonsfrom clinical data and BMT models.2011
Author(s)
Kato, N., Kitaura, J., Doki, N., Komeno, Y., Watanabe-Okochi, N., Togami, K., Nakahara, F., Oki, T., Enomoto, Y., Fukuchi, Y., Nakajima, H., Harada, Y., Harada, H., Kitamura, T.
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Journal Title
Blood. 117
Pages: 221-233
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[Journal Article] Hes1 immortalizes committed progenitors and plays a role in blastic crisis of chronic myelogenous leukemia.2010
Author(s)
Nakahara, F., Sakata-Yanagimoto, M., Komeno, Y., Kato, N., Haraguchi, K., Kumano, K., Harada, Y., Harada, H., Kitaura, J., Ogawa, S., Kurokawa, M., Kitamura, T., Chiba, S.
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Journal Title
Blood. 115
Pages: 2872-2881
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[Journal Article] Expression of ADAMTS4 in Ewing's sarcoma.2010
Author(s)
Minobe, K., Ono, R., Matsumine, A., Shibata-Minoshima, F., Izawa, K., Oki, T., Kitaura, J., Iino, T., Takita, J., Iwamoto, S., Hori, H., Komada, Y., Uchida, A., Hayashi, Y., Kitamura, T., Nosaka, T.
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Journal Title
Int J Oncol. 37
Pages: 569-581
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[Journal Article] AID-induced T-lymphoma or B-leukemia/lymphoma in a mouse BMT model.2010
Author(s)
Komeno, Y., Kitaura, J., Watanabe-Okochi, N., Kato, N., Oki, T., Nakahara, F., Harada, Y., Harada, H., Shinkura, R., Nagaoka, H., Hayashi, Y., Honjo, T., Kitamura, T.
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Journal Title
Leukemia. 24
Pages: 1018-1024
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[Journal Article] TIM1 is an endogenous ligand for LMIR5/CD300b : LMIR5 deficiency ameliorates mouse kidney ischemia/reperfusion injury.2010
Author(s)
Yamanishi, Y., Kitaura, J., Izawa, K., Kaitani, A., Komeno, Y., Nakamura, M., Yamazaki, S., Enomoto, Y., Oki, T., Akiba, H., Abe, T., Komori, T., Morikawa, Y., Kiyonari, H., Takai, T., Okumura, K., Kitamura, T.
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Journal Title
J Exp Med. 207
Pages: 1501-1511
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[Journal Article] Characterization of leukocyte mono-immunoglobulin-like receptor 7 (LMIR7)/CLM-3 as an activating receptor : its similarities to and differences from LMIR4/CLM-5.2010
Author(s)
Enomoto, Y., Yamanishi, Y., Izawa, K., Kaitani, A., Takamatsu, R., Kajikawa, M., Takai, T., Kitamura, T., Kitaura, J.
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Journal Title
J Bio Chem. 285
Pages: 35274-35283
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[Journal Article] Enhanced expression of p210BCR/ABL and aberrant expression of Zfp423/ZNF423 induce blast crisis of chronic myelogenous leukemia.2009
Author(s)
Miyazaki, K., Yamasaki, N., Oda, H., Kuwata, T., Kanno, Y., Miyazaki, M., Komeno, Y., Kitaura, J., Honda, Z., Warming, S., Jenkins, N.A., Copeland, N.G., Kitamura, T., Nakamura, T., Honda, H.
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Journal Title
Blood. 113
Pages: 4702-4710
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[Journal Article] Possible involvement of RasGRP4 in leukemogenesis.2009
Author(s)
Watanabe-Okochi, N., Oki, T., Komeno, Y., Kato, N., Yuji, K., Ono, R., Harada, Y., Harada, H., Hayashi, Y., Nakajima, H., Nosaka, T., Kitaura, J., Kitamura, T.
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Journal Title
Int J Hematol. 89
Pages: 470-481
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[Journal Article] An activating and inhibitory signal from an inhibitory receptor LMIR3/CLM-1 : LMIR3 augments lipopolysaccharide response through association with FcRgamma in mast cells.2009
Author(s)
Izawa, K., Kitaura, J., Yamanishi, Y., Matsuoka, T., Kaitani, A., Sugiuchi, M., Takahashi, M., Maehara, A., Enomoto, Y., Oki, T., Takai, T., Kitamura, T.
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Journal Title
J Immunol. 183
Pages: 925-936
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[Journal Article] Evidence that integrin τIIbυ3-dependent interaction of mast cells with fibrinogen exacerbates chronic inflammation.2009
Author(s)
Oki, T., Eto, K., Izawa, K., Yamanishi, Y., Inagaki, N., Frampton, J., Kitamura, T., Kitaura, J.
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Journal Title
J Bio Chem. 284
Pages: 31463-31472
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[Journal Article] Pivotal advance: IgE accelerates in vitro development of mast cells and modifies their phenotype.2008
Author(s)
Kashiwakura, J., Xiao, W., Kitaura, J., Kawakami, Y., Maeda-Yamamoto, M., Pfeiffer, J.R., Wilson, B.S., Blank, U., Kawakami, T.
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Journal Title
J Leukoc Biol. 84
Pages: 357-367
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[Journal Article] AML1 mutations induced MDS and MDS/AML in a mouse BMT model.2008
Author(s)
Watanabe-Okochi, N., Kitaura, J., Ono, R., Harada, H., Harada, Y., Komeno, Y., Nakajima, H., Nosaka, T., Inaba, T., Kitamura, T.
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Journal Title
Blood. 111
Pages: 4297-4308
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