Exploration of possible catabolic factors in osteoarthritis through the analysis of large numbers of human samples.

2010 Fiscal Year Final Research Report

• Project/Area Number: 20390410
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Clinical Research Center for Allergy and Rheumatology, National Hospital Organization, Sagamihara National Hospital
• Principal Investigator: FUKUI Naoshi Clinical Research Center for Allergy and Rheumatology, National Hospital Organization, Sagamihara National Hospital, 病態総合研究室, 部長 (10251258)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Ryuji 独立行政法人国立病院機構(相模原病院臨床研究センター), 診断・治療研究室, 室長 (70373470) ; MITOMI Hiroyuki 独立行政法人国立病院機構(相模原病院臨床研究センター), 病態総合研究部, 研究員 (90181940)
• Project Period (FY): 2008 – 2010
• Keywords: 変形性関節症 / 軟骨変性因子

Research Abstract

In this project, we have obtained synovial and cartilage tissues and synovial fluid samples from a large number of knee OA patients at various disease severities, and conducted a series of gene expression and protein analyses. Through the analyses, we have constructed a data base about human OA tissues and synovial fluid, which we found highly useful in exploration of possible catabolic factors for cartilage in the disease. Utilizing this data base, we were able to identify several possible catabolic factors in OA. Through a series of analyses with the data, we have identified a possible mechanism for synovial changes in OA. Validation of the significance of these factors in the disease is a future problem which should be answered by subsequent studies.

Research Products

• [Journal Article] Aberrant p16^INK4a methylation is a frequent event in colorectal cancers : prognostic value and relation to mRNA expression and immunoreactivity. (2010)
  • Author(s): Mitomi H, Fukui N, Tanaka N, Kanazawa H, Saito T, Matsuoka T, Yao T.
  • Journal Title: J Cancer Res Clin Oncol 136 Pages: 323-331
• [Journal Article] Communication skills training on how to break bad news for Japanese nurses in oncology : effects of training on nurses' confidence and perceived effectiveness. (2010)
  • Author(s): Fukui S, Ogawa K, Fukui N.
  • Journal Title: J Cancer Educ 25 Pages: 116-119
• [Journal Article] New sequence variants in HLA class II/III region associated with susceptibility to knee osteoarthritis identified by genome-wide association study. (2010)
  • Author(s): Nakajima M, Takahashi A, Kou I, Rodriguez-Fontenla C, Gomez-Reino JJ, Furuichi T, Dai J, Sudo A, Uchida A, Fukui N, Kubo M, Kamatani N, Tsunoda T, Malizos KN, Tsezou A, Gonzalez A, Nakamura Y, Ikegawa S.
  • Journal Title: PLoS One 5 Pages: 9723
• [Journal Article] Changes of human menisci in osteoarthritic knee joints. (2010)
  • Author(s): Katsuragawa Y, Saitoh K, Tanaka N, Wake M, Ikeda Y, Furukawa H, Tohma S, Sawabe M, Ishiyama M, Yagishita S, Suzuki R, Mitomi H, Fukui N.
  • Journal Title: Osteoarthritis and Cartilage 18 Pages: 1133-1143
• [Journal Article] Relationship between radiographic changes and symptoms or physical examination findings in subjects with symptomatic medial knee osteoarthritis : a three-year prospective study. (2010)
  • Author(s): Fukui N, Yamane S, Ishida S, Tanaka K, Masuda R, Tanaka N, Katsuragawa Y, Fukui S.
  • Journal Title: BMC Muscloskelet Disord 11 Pages: 269-
• [Journal Article] Addition of an arch support improves the biomechanical effect of a laterally wedged insole. (2009)
  • Author(s): Nakajima K, Kakihana W, Nakagawa T, Mitomi H, Hikita A, Suzuki R, Akai M, Iwaya T, Nakamura K, Fukui N.
  • Journal Title: Gait and Posture 29 Pages: 208-213
• [Journal Article] Effect of communication skills training on nurses' detection of patient distress and related factors after cancer diagnosis : A randomized study. (2009)
  • Author(s): Fukui S, Ogawa K, Ohtsuka M, Fukui N.
  • Journal Title: Psycho-Oncology 18 Pages: 1156-1164
• [Journal Article] The interaction of monocytes with rheumatoid synovial cells is a key step in LIGHT-mediated inflammatory bone destruction. (2009)
  • Author(s): Ishida, S, Yamane S, Nakano S, Yanagimoto T, Hanamoto Y, Maeda-Tanimura M, Toyosaki-Maeda T, Ishizaki J, Matsuo Y, Fukui N, Itoh T, Ochi T, Suzuki R.
  • Journal Title: Immunology 128 (1 Suppl) Pages: 315-24
• [Journal Article] Involvement of a disintegrin and metalloproteinase 10 and 17 in shedding of tumor necrosis factor-α. (2009)
  • Author(s): Hikita A, Tanaka N, Yamane S, Ikeda Y, Furukawa H, Tohma S, Suzuki R, Tanaka S, Mitomi H, Fukui N.
  • Journal Title: Biocehm Cell Biol 87 Pages: 581-593
• [Journal Article] LIGHT induces cell proliferation and inflammatory responses of rheumatoid arthritic synovial fibroblasts via lymphotaxin β receptor. (2008)
  • Author(s): Ishida S, Yamane S, Ochi T, Nakano S, Mori T, Juji T, Fukui N, Itoh T, Suzuki R.
  • Journal Title: J Rheumatol 35 Pages: 960-968
• [Journal Article] Common variants in DVWA on chromosome 3p24.3 are associated with susceptibility to knee osteoarthritis. (2008)
  • Author(s): Miyamoto Y, Shi D, Nakajima M, Ozaki K, Sudo A, Kotani A, Uchida A, Tanaka T, Fukui N, Tsunoda T, Takahashi A, Nakamura Y, Jiang Q, Ikegawa S.
  • Journal Title: Nat Genet 40 Pages: 994-998
• [Journal Article] Zonal gene expression of chondrocytes in osteoarthritic cartilage. (2008)
  • Author(s): Fukui N, Miyamoto Y, Nakajima M, Ikeda Y, Hikita A, Furukawa H, Mitomi H, Tanaka N, Katsuragawa Y, Yamamoto S, Sawabe M, Juji T, Mori T, Suzuki R, Ikegawa S.
  • Journal Title: Arthritis Rheum 58 Pages: 3843-3853
• [Journal Article] A randomized study assessing efficacy of communication skill training on patients' psychologic distress and coping : Nurses' communication with patients just after being diagnosed with cancer. (2008)
  • Author(s): Fukui S, Ogawa K, Ohtsuka M, Fukui N.
  • Journal Title: Cancer 113 Pages: 1462-1470
• [Journal Article] Inhibition of Src homology 2 domain-containing protein tyrosine phosphatase substrate-1 reduces the severity of collagen-induced arthritis. (2008)
  • Author(s): Tanaka K, Horikawa T, Suzuki S, Kitaura K, Watanabe J, Gotoh A, Shiobara N, Yamane S, Suzuki R, Fukui N, Ochi T.
  • Journal Title: J Rheumatol 35 Pages: 2316-2324
• [Presentation] Exploration of possible catabolic factors for cartilage matrix in osteoarthritic synovial fluid. (2010)
  • Author(s): Fukui N, Ikeda Y, Tanaka N, Wake M, Ohmori M, Yamaguchi T, Furukawa H, Touma S, Miyamoto Y, Tashiro T, Katsuragawa Y.
  • Organizer: 2010 World Congress on Osteoarthritis.
  • Place of Presentation: Brussels, Belgium.
  • Year and Date: 20100923-20100926
• [Presentation] Dedifferentiation of monolayer cultured chondrocytes could be promoted by αvβ5 and α5β1 integrins. (2010)
  • Author(s): Fukui N.
  • Organizer: Osteoarthritis Research Society International, 2010 China Forum.
  • Place of Presentation: Nanjing, China.
  • Year and Date: 20100923-20100926
• [Presentation] Altered chondrocyte metabolism in osteoarthritic cartilage. Can it be normalized?. (2008)
  • Author(s): Fukui N.
  • Organizer: 13th Congress on the Asia Pacific League of Associations for Rheumatology.
  • Place of Presentation: Yokohama.
  • Year and Date: 20080923-20080927
• [Presentation] Addition of an arch support improves the biomechanical effect of a laterally wedged insole. (2008)
  • Author(s): Nakajima K, Kakihana W, Nakagawa T, Mitomi H, Hikita A, Suzuki R, Akai M, Iwaya T, Nakamura K, Fukui N.
  • Organizer: 2008 World Congress on Osteoarthritis.
  • Place of Presentation: Roma, Italy
  • Year and Date: 20080918-20080921
• [Presentation] Zonal gene expression of chondrocytes in osteoarthritis cartilage. (2008)
  • Author(s): Fukui N, Miyamoto Y, Nakajim M, Ikeda Y, Hikita A, Furukawa H, Mitomi H, Tanaka N, Katsuragawa Y, Yamamoto S, Sawabe M, Juji T, Mori T, Suzuki R, Ikegawa S.
  • Organizer: 2008 World Congress on Osteoarthritis.
  • Place of Presentation: Roma, Italy.
  • Year and Date: 20080918-20080921
• [Book] In : Laser capture microdissection : methods and applications. 2nd Ed. Murray GI, editor. London : Springer-Verlag
  • Author(s): Fukui N, Ikeda Y, Tanaka N.
  • Publisher: The use of laser capture microdissection on adult human articular cartilage for gene expression analysis.(in press.)
• [Remarks] ホームページ等
  • URL: http://www.hosp.go.jp/~sagami/rinken/crc/index.html