2010 Fiscal Year Final Research Report
The biological characteristics of amniotic membrane and it's application for regenerative medicine.
| Project/Area Number |
20390430
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | University of Toyama |
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Principal Investigator |
NIKAIDO Toshio University of Toyama, 大学院・医学薬学研究部(医学), 教授 (50180568)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Tosiko 富山大学, 大学院・医学薬学研究部, 准教授 (00171421)
OKABE Motonori 富山大学, 大学院・医学薬学研究部, 助教 (60283066)
KOIKE Cika 富山大学, 大学院・医学薬学研究部, 助教 (10523889)
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| Co-Investigator(Renkei-kenkyūsha) |
SAITO Sigeru 富山大学, 大学院・医学薬学研究部, 教授 (30175351)
KITAGAWA Kiyotaka 富山大学, 大学院・医学薬学研究部, 准教授 (00270950)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 産科学 / 再生 / 羊膜 / 上皮細胞 / 間葉系細胞 |
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| Research Abstract |
1) Amniotic epithelial cell, mesenchymal cell and umbilical mesenchymal cell, cultured under ultra low attached dish, made spheres that had self-renewal ability, expression of mRNA or proteins of stem cell markers (Nanog, Oct3/4, SSEA4).
2) We made 11 immortalized cell lines of amniotic epithelial cell, mesenchymal cell, umbilical mesenchymal cells, which work were collaborated with Prof. Kiyono (National Cancer Center). Those cell lines were made by virus-transfection of gene such as E6E7, hTERT, bmil and cyclin inhibitor. We selected some cell lines that did not change cell growth and morphology even though over 50 PDL from these 11 lines. Those selected cell lines differentiated into bone, cartilage and adipose cells.
3) Dried amniotic membrane were used for operation of the pterygium and aqueous humor tunnel plasty under the glaucoma. Under these treatment, shortening of the operative time, available for the large tissue defect were shown as advantages.
4) i) The epithelial surface of dried amniotic membrane suppress for adhesion with the tissue, and stromal surface adherenced to tissue. And the dried amniotic membrane worked as a good scaffold even if the surface was epithelium. The conjunctiva cells grew and multiplied on the dried amniotic membrane which we transplanted in the lesion. ii) It was easy to handle and make the dried amniotic membrane for the suitable size and shape that it was compared with fresh or cryopreserved amniotic membrane, and wrinkles were hard to occur. These advantages become that an ophthalmologist could be short the operative time, could be available for the large tissue defect. iii) We developed a polymer to solubilize hydrophobic antiviral medication for treatment keratitis herpetic. We impregnated the solubilized antiviral medication to dried amniotic membrane, and presented with a similar effect that of treatment with the ointment in rabbit model.
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[Journal Article] A role for IL-17 in induction of an inflammation at the fetomaternal interface in preterm labour.2010
Author(s)
Ito M., Nakasima A., Hidaka T., Okabe M., Bac ND., Ina S., Yoneda S., Shiozaki A., Sumi S., Tuneyama K., Nikaido T., Saito S.
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Journal Title
J Reprod Immunol 84
Pages: 75-85
Peer Reviewed
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[Journal Article] Mutation-, Aging-, and Gene Dosage-dependent Accumulation of Neuroserpin (G392E) in Endoplasmic Reticula and Lysosomes of Neurons in Transgenic Mice.2008
Author(s)
Takasawa A., Kato I., Takasawa K., Ishii Y., Yoshida T., Shehata M., Kawaguchi H., Mohafez O., Sasahara M., Hiraga K.
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Journal Title
Journal of Biological Chemistry 283
Pages: 35606-35613
Peer Reviewed
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[Journal Article] Strom SC.Isolation and Characterization of Cells from Human Term Placenta. Outcome of the First International Workshop on Placenta Derived Stem Cells.2008
Author(s)
Parolini O., Alviano F., Bagnara GP., Bilic G., Buhring H-J., Evangelista M., Hennerbichler S., Liu B., Magatti M., Mao N., Miki T., Marongiu F., Nakajima H., Nikaido T., Portmann-Lanz C-B., Sankar V., Soncini M., Stadler G., Surbek D., Takahashi T., Redle H., Sakuragawa N., Wolbank S., Zeisberger S., Zisch A.
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Journal Title
Stem Cells 26
Pages: 300-311
Peer Reviewed
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[Presentation] マウス羊膜幹細胞の分化能2009
Author(s)
吉田淑子, 籐賛, 周凱旋, 岡部素典, 小池千加, 米田徳子, 野上真紀子, 樋口収, 二階堂敏雄
Organizer
第8回日本再生医療学会総会
Place of Presentation
東京
Year and Date
20090305-20090306
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[Presentation] Establishment AND Characterization of an Immortalized Human Amniotic Membrane Cells.2008
Author(s)
Teng Z., Toda A., Yoshida T., Okabe M., Higuchi O., Nogami M., Yoneda N., Zhou K., Kyo S., Kiyono T., Nikaido T.
Organizer
第7回日本再生医療学会総会
Place of Presentation
名古屋
Year and Date
20080313-20080314
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[Presentation] 羊膜間葉系細胞の骨分化誘導2008
Author(s)
津野宏彰, 吉田淑子, 野上真紀子, 岡部素典, 野口誠, 京哲, 清野透, 二階堂敏雄
Organizer
第7回日本再生医療学会総会
Place of Presentation
名古屋
Year and Date
20080313-20080314
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[Presentation] Immortalized Human Amniotic Epithelial Cells maintained the characteristics of stem cells.2008
Author(s)
Zhou K., Toda A., Teng Z., Yoshida T., Okabe M., Higuchi O., Nogami M., Yoneda N., Kyo S., Kiyono T., Nikaido T.
Organizer
第7回日本再生医療学会総会
Place of Presentation
名古屋
Year and Date
20080313-20080314
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[Presentation] 褥創モデルラットを用いた不死化羊膜間葉細胞添加Collagen gelによる創傷治癒効果の検討2008
Author(s)
高橋雅法, 吉田淑子, 岡部素典, 木村真梨, 籐賛, 戸田文香, 米田徳子, 京哲, 清野透, 柴原直利, 二階堂敏雄
Organizer
第7回日本再生医療学会総会
Place of Presentation
名古屋
Year and Date
20080313-20080314
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