2011 Fiscal Year Final Research Report
Functional analysis for molecular interaction through a new member of the immunoglobulin superfamily in tooth germ development
| Project/Area Number |
20390466
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Ieyoshi 九州大学, 歯学研究院, 准教授 (40243951)
KIYOSHIMA Tamotsu 九州大学, 歯学研究院, 講師 (20264054)
NAGATA Kengo 九州大学, 歯学研究院, 助教授 (90189134)
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| Project Period (FY) |
2008 – 2011
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| Keywords | 口腔病理学 / 歯の発生・再生 / 歯原性幹細胞 / 器官培養 |
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| Research Abstract |
Protogenin(Prtg) has been identified as a gene that is highly expressed in the mouse mandible at embryonic day 10. 5(E10. 5). Prtg is a new member of the deleted in colorectal carcinoma(DCC) family. This study examined the functional implications of Prtg in the developing tooth germ of the mouse lower first molar. Ptrg is preferentially expressed in the early stage of organogenesis. Prtg was widely expressed in the mesenchymal and oral epithelial cells in the E10. 5 mandible. The expression of Prtg after E12. 0 was markedly reduced in the mandible, and was restricted to the area where the tooth bud was likely to be formed. An antisense inhibition assay for Prtg in cultured E10. 5 mandibles showed a significant growth inhibition in the tooth germ. Bmp-4 expression had significantly been decreased in the mouse E10. 5 mandible at 24 hr after Prtg inhibition. These results indicated that the Prtg might be related to the initial morphogenesis of the tooth germ in the mouse lower first molar. In addition, in situ expression of Ifrg15, L21 and ATPase6 genes suggest that these genes participate in the initiation and the development of the tooth germ as well as those of the other craniofacial organs. Other examination results also demonstrated that Tβ4 is involved in the early stage of tooth germ development by regulation of the odontogenesis-related gene expression.
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[Journal Article] Protogenin, a new member of the immunoglobulin superfamily2010
Author(s)
Takahashi KF, Kobayashi I, Xie Kiyoshima M, Yamaza T, H, Fujiwara H, Ookuma Y, Nagata K, Wada H, Sakai T, Terada Y, and Sakai H
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Journal Title
BMC Dev Biol
Volume: Vol.10 No.115
Peer Reviewed
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