2012 Fiscal Year Final Research Report
Interaction between odontoblastic syncytium coupled through gap-junction and pulpal nervous and circulatory system
| Project/Area Number |
20390482
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
IKEDA Hideharu 東京医科歯科大学, 歯学部附属病院, 講師 (20222896)
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| Co-Investigator(Kenkyū-buntansha) |
SUDA Hideaki 東京医科歯科大学, 大学院・医歯学総合研究科, 教授 (00114760)
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| Research Collaborator |
BRUCE Matthews 連合王国ブリストル大学, 医学研究所, 教授
MATTHEW Holley 連合王国シェフィールド大学, 生物学研究所, 教授
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| Project Period (FY) |
2008 – 2012
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| Keywords | 象牙芽細胞 / ギャップ結合 / イオンチャンネル / 電気カップリング / TRP チャンネル / 歯髄リンパ管 / transjunctional conductance / 歯髄炎 |
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| Research Abstract |
We electrophysiologically showed direction-independent gap-junctionalcommunication between odontoblasts and direction dependent gap-junction betweenodontoblasts and underneath pulpal cells for the first time. These communicationsmay form a functional syncytium in the odontoblastic layer.
So-called “the hydrodynamic theory” is based on the fluid movement in the dentinaltubules. However, their inner diameters are much larger than those in the enamel side.That is, hydrodynamic pressure could not be elevated due to this large diameter oftubules. We measured the functional space between odontoblast processes and the wallof dentinal tubules using fluorescent microspheres with different diameters andexcitation wave lengths. Small microspheres (<0.04μm) could reach the pulp. Thissmall space may contribute to the high hydrodynamic pressure that trigger actionpotentials in afferent terminals and activate mechanosensitive ion channels inodontoblast cell membrane.
Adrenergic β2 receptors are associated with bone formation. We found β2 receptorimmunoreactivity in the human odontoblastic layer. We also found theimmunoreactivity of cold sensitive TRP channels and lymphatic vessels. Thus,interactions among blood and lymphatic circulation, cold receptiveness, andsympathetic β2 receptors, and may contribute to dentinogenesis.
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[Book] Quintessence Int Publishing2012
Author(s)
Ikeda H, Suda H
Total Pages
全501ページ
Publisher
2nd edition, Eds Hargreaves K, Goodis H, Seltzer and Bender's The Dental Pulp, Chapter 6 Circulation of the Pulp
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