2010 Fiscal Year Final Research Report
The discovery of molecules that inhibit oral biofilm formation by inhibiting cell-to-cell communication
| Project/Area Number |
20390537
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Social dentistry
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
TAKEHARA Tadamichi Kyushu Dental College, 歯学部, 名誉教授 (00038879)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Akihiro 九州歯科大学, 歯学部, 助教 (20364151)
ANSAI Toshihiro 九州歯科大学, 歯学部, 准教授
AWANO Shuji 九州歯科大学, 歯学部, 講師 (20301442)
SOH Inho 九州歯科大学, 歯学部, 助教 (10285463)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 細菌間シグナル / クオラムセンシング / バイオフィルム / 口腔細菌 / 阻害剤 |
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| Research Abstract |
We initially produced several analogues by modifying the chemical structures of autoinducer-2 (AI-2). We examined the inhibitory activities of these analogues by examining the luminescence activities of Vibrio harveyi, a luminescent marine bacterium species. Of the analogues synthesized, we chose those that inhibited the luminescence activities of V.harveyi. Using the selected analogues, we analyzed the inhibitory effects of these analogues on biofilm formation by oral streptococci, including Streptococcus mutans, a human cariogenic species. Some of the analogues inhibited biofilm formation by these bacteria, although some of the analogues that inhibited biofilm formation by S. mutans did not inhibit biofilm formation by other streptococci. We could not find a common inhibitor for all oral streptococci. Now we are analyzing the role of these analogues in the expression of S.mutans glucosyltransferase (GTF) genes and their function in biofilm formation by S.mutans.
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[Journal Article] Streptococcus anginosus L-cysteine desulfhydrase gene expression is associated with abscess formation in BALB/c mice.2011
Author(s)
Takahashi, Y., Yoshida, A., Nagata, E., Hoshino, T., Oho, T., Awano, S., Takehara, T., Ansai, T.
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Journal Title
Molecular Oral Microbiol. 26
Pages: 221-227
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[Journal Article] Rapid LED-based fluorescence microscopy distinguishes between live and dead bacteria in oral clinical samples.2010
Author(s)
Masakiyo, Y., Yoshida, A., Takahashi, Y., Shintani, Y., Awano, S., Ansai, T., Sawayama, S., Shimakita, T., Takehara, T.
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Journal Title
Biomed Res. 31
Pages: 21-26
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[Journal Article] The identification of genes specific to Prevotella intermedia and Prevotella nigrescens using genomic subtractive hybridization.2010
Author(s)
Masakiyo, Y., Yoshida, A., Shintani, Y., Takahashi, Y., Ansai, T., Takehara, T.
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Journal Title
Anaerobe 16
Pages: 265-269
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[Journal Article] Hydrogen sulfide production from cysteine and homocysteine by periodontal and oral bacteria.2009
Author(s)
Yoshida, A., Yoshimura, M., Ohara, N., Yoshimura, S., Nagashima, S., Takehara, T., Nakayama, K.
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Journal Title
J Periodontol. 80
Pages: 1845-1851
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[Journal Article] Identification of the genes associated with a virulent strain of Porphyromonas gingivalis using the subtractive hybridization technique.2008
Author(s)
Tachibana-Ono, M., Yoshida, A., Kataoka, S., Ansai, T., Shintani, Y., Takahashi, Y., Toyoshima, K., Takehara, T.
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Journal Title
Oral Microbiol. Immunol. 23
Pages: 84-87
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[Presentation] The prevalence ofperiodontopathic bacteriain patients withchronic kidney disease.2009
Author(s)
Yoshida A., Awano S., Ansai T., Masakiyo, Teratani, G., Soh, I., Hamasaki, T., Takehara T.
Organizer
The 9th WorldCongress on Preventive Dentistry
Place of Presentation
Phuket, Thailand
Year and Date
20091107-20091110
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