2010 Fiscal Year Final Research Report
Intracellular Dynamics of a drug by Raman microscopy
| Project/Area Number |
20500396
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
HARADA Yoshinori Kyoto Prefectural University of Medicine, 医学研究科, 助教 (10381956)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAOKA Yoshihisa 京都府立医科大学, 医学研究科, 助教 (80405274)
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| Project Period (FY) |
2008 – 2010
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| Keywords | バイオイメージング / ラマン分光 / 薬物代謝 / 細胞 |
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| Research Abstract |
Since Raman scattering light measurement does not require pretreatment, biochemical molecules in their innate state can be analyzed where no exogenous probes are added. However, imaging analyses of biological events in living samples by using Raman scattering light measurement have been relatively unsuccessful because of low Raman scattering efficiency and the required long measurement time. In this study, we attempted to visualize drug distribution in living cancer cells treated with an anticancer reagent CPT-11 by using high-speed Raman microscopy. We also analyzed intracellular conversion of CPT-11 to its metabolite SN-38 using Raman spectra.
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[Journal Article] Evaluation of autofluorescence colonoscopy for diagnosis of superficial colorectal neoplastic lesions.2010
Author(s)
Inoue K, Wakabayashi N, Morimoto Y, Miyawaki K, Kashiwa A, Yoshida N, Nakano K, Takada H, Harada Y, Yagi N, Naito Y, Takamatsu T, Yoshikawa T.
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Journal Title
Int J Colorectal Dis 25
Pages: 811-816
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