The role of chaperones and micro RNA on regulation of radiation-induced mutation in human physiological functions.

2010 Fiscal Year Final Research Report

• Project/Area Number: 20510048
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Chiba University
• Principal Investigator: SUZUKI Nobuo Chiba University, 大学院・医学研究院, 教授 (90111426)
• Co-Investigator(Kenkyū-buntansha): KITA Kazuko 千葉大学, 大学院・医学研究院, 講師 (80302545) ; SUGAYA Shigeru 千葉大学, 大学院・医学研究院, 助教 (90334177) ; SUZUKI Toshikazu 千葉大学, 大学院・医学研究院, 助教 (70270527)
• Project Period (FY): 2008 – 2010
• Keywords: ヒト / 放射線 / 変異 / シャペロン / プロテアーゼ / 血清 / 細胞 / DNA修復

Research Abstract

The following two points were mainly identified in mechanisms which regulate mutability in human. (1) When the HSP27 and annexin II complex is involved in DNA repair pathways for UVC-caused DNA damages, HSP27 may protect annexin II from the breakdown after UVC irradiation. Annexin II is released from cells and the released one may be involved in cellular UVC-resistance via signal transduction pathways. (2) The miRNAs, which may be involved in cell growth and mutation, were found.

Research Products

• [Journal Article] Screening of novel peptide ligands bound to the carboxy-terminal domain of GRP94 by phage display (2011)
  • Author(s): Zahed M., Suzuki T., Kita K., Sugaya S., Suzuki N.
  • Journal Title: Chiba Medical Journal 87 Pages: 61-68
• [Journal Article] Studies on the amounts of serum hydroperoxide, MMP-3, urinary 8-OHdG, and salivary IgA in rheumatoid arthritis who experienced Shinrin-yoku (forest-air bathing and walking) (2011)
  • Author(s): Sugaya S., Tanaka K., Akagi T., Kasetani T., Qiu-Ji Z., Guo W-Z., Udagawa A., Sugita K., Ohta R., Suzuki N.
  • Journal Title: Chiba Medical Journal 87 Pages: 5
• [Journal Article] Combinatory cytotoxic effects produced by E1B-55kDa-deleted adenoviruses and chemotherapeutic agents are dependent on the agents in esophageal carcinoma.Detail Only Available (2010)
  • Author(s): Ma G., Kawamura K., Li Q., Okamoto S. Suzuki N., Kobayashi H., Liang M., Tada Y., Tatsumi K., Hiroshima K., Shimada H., Tagawa M.
  • Journal Title: Cancer Gene Therapy 17 Pages: 803-813
• [Journal Article] Anti-proliferative and apoptosis-inducible activity of labdane and abietane diterpenoids from the pulp of Torreya nucifera in HeLa cells (2010)
  • Author(s): Chen S-P., Dong M., Kita K., Shi Q-W., Cong B., Guo W-Z., Sugaya S., Sugita K., Suzuki N.
  • Journal Title: Mol.Med.Rep. 3 Pages: 673-678
• [Journal Article] Interferon-λ induces G1 phase arrest or apoptosis in oesophageal carcinoma cells and produces anti-tumour effects in combination with anti-cancer agents (2010)
  • Author(s): Li Q., Kawamura K., Ma G., Iwata F., Numasaki M., Suzuki N., Shimada H., Tarawa M.
  • Journal Title: European Journal of Cancer 46 Pages: 180-190
• [Journal Article] Xylomexicanins A and B, New △14,15-Mexicanolides from Seeds of the Chinese Mangrove Xylocarpus granatum (2009)
  • Author(s): Li-Ru Shen, Mei Dong, Dong Guo, Bao-Wei Yin, Man-Li Zhang, Qing-Wen Shi, Chang-Hong Huo, Hiromasa Kiyotad, Shuzuki N., Bin Cong.
  • Journal Title: Z.Naturforsch 64c Pages: 37-42
• [Journal Article] Screening of SMG7-Binding Peptides by Combination of Phage Display and Docking Simulation Analysis (2009)
  • Author(s): Zahed M., Suzuki T., Suganami A., Sugiyama H., Harada K., Takiguchi M., Tamura Y., Suzuki N.
  • Journal Title: Protein Peptide Lett 16 Pages: 301-5
• [Journal Article] Cyclicalcell stretching of skin-derived fibroblasts downregulates connective tissue growth factor (CTGF) production (2009)
  • Author(s): Kanazawa Y., Nomura J., Yoshimoto S., Suzuki T., Kita K., Suzuki N., Ichinose, M.
  • Journal Title: Connective Tissue Res. 50 Pages: 323-329
• [Journal Article] Luteolin increases the expression of GRP94 in HeLa cells (2009)
  • Author(s): Hu G., Suzuki T., Dong M., Shi Q.-W., Cong B., Suzuki N.
  • Journal Title: Biosci.Biotec.Biochem. 73 Pages: 2341-2344
• [Journal Article] Anti-proliferative and apoptosis-inducible activity of Sarcodonin G from Sarcodon scabrosus in HeLa cells (2009)
  • Author(s): Dong M., Chen S.P., Kita K., Ichimura Y., Guo W-Z., Lu S., Sugaya S., Hiwasa T., Takiguchi M., Mori N., Kashima A., Morimura K., Hirota M., Suzuki N.
  • Journal Title: Int.J.Oncol. 34 Pages: 201-207
• [Journal Article] The roles of HSP27 and annexin II in resistance to UVC-induced cell death comparative studies between the human UVC-sensitive and -resistant cell lines, RSa and APr-1 (2009)
  • Author(s): Jin Y-H., Kita K., Sun Z., Tong X-B., Nie H., Suzuki N.
  • Journal Title: Biosci.Biotec.Biochem. 73 Pages: 1318-22
• [Journal Article] Cytotoxicity of adenoviruses expressing the wild-type p53 gene to esophageal carcinoma cells is linked with the CAR expression level and indirectly with the endogenous p53 status (2009)
  • Author(s): Ma G., Kawamura K., Li Q., Suzuki N., Liang M., Namba M., Shimada H., Tarawa M.
  • Journal Title: Cancer Gene Therapy 16 Pages: 832-840
• [Journal Article] Increase in the levels of chaperone proteins by exposure to β-estradiol, bisphenol A and 4-methoxyphenol in human cells transfected with estrogen receptor cDNA (2009)
  • Author(s): Kita K., Jin Y-H., Sun Z., Chen S-P., Sumiya Y., Hongo T., Suzuki N.
  • Journal Title: Toxicology in Vitro 23 Pages: 728-735
• [Journal Article] Nm23-H1 is responsible for SUMO-2-involved DNA synthesis induction after X-ray irradiation in human cells (2009)
  • Author(s): Guo W., Sugaya S., Satoh M., Tomonaga T., Nomura F., Hiwasa T., Kita K., Takiguchi M., Suzuki N.
  • Journal Title: Archives of Biochemistry and Biophysics 486 Pages: 81-87
• [Journal Article] Gene medicine for cancer treatment : Commercially available medicine and accumulated clinical data in China (2008)
  • Author(s): Ma G., Shimada H., Hiroshima K., Tada Y., Suzuki N., Tagawa M.
  • Journal Title: Drug Design, Development and Therapy 2 Pages: 115-122
• [Journal Article] Stable knockdown of PASG enhances DNA demethylation but does not accelerate cellular senescence in TIG-7 human fibroblasts (2008)
  • Author(s): Suzuki T., Farrar JE., Yegnasubramanian S., Zahed M., Suzuki N., Arceci RJ.
  • Journal Title: Epigenetics 3 Pages: 281-291
• [Journal Article] Involvement of glyceraldehyde-3-phosphate dehydrogenase in X-ray resistance of HeLa cells (2008)
  • Author(s): Lu J., Suzuki T., Lu S., Suzuki N.
  • Journal Title: Biosci.Biotec.Biochem. 72 Pages: 2432-2435
• [Journal Article] a novel HSP27-interacted protein, is involved in resistance to UVC-induced cell death in human APr-1 cells (2008)
  • Author(s): Tong X., Kita K., Karata K., Zhu C., Sugaya S., Ichimura Y., Satoh M., Tomonaga T., Nomura F., Jin Y., Suzuki N. Annexin II
  • Journal Title: Photochem.Photobiol. 84 Pages: 1455-1461
• [Journal Article] Involvement of aldolase A in X-ray resistance of human HeLa and UVr-1 cells. (2008)
  • Author(s): Lu J., Suzuki T., Satoh M., Chen S., Tomonaga T., Nomura F., Suzuki N.
  • Journal Title: Biochem.Biophys.Res.Commun. 369 Pages: 948-952
• [Journal Article] Discrepancy of Cadmium Susceptibility Between Human RSa Cells and Variant UVr-1 Cells (2008)
  • Author(s): Ji Z-q., Sugaya S., Suzuki N.
  • Journal Title: Chiba Med.J. 84 Pages: 75-82
• [Journal Article] Down-regulation of Molecular Chaperone GRP78/Bip Expression Involved in Enhancement of Human RS Cell Mutability (2008)
  • Author(s): Hirano J., Kita K., Sugaya S., Ichimura Y., Yamamori H., Nakajima N., Suzuki N.
  • Journal Title: Pancreas 36 Pages: e7-14
• [Journal Article] Cancer therapy with local oncolysis and topical cytokine secretion (2008)
  • Author(s): Tagawa M., Kawamura K., Ueyama T., Nakamura N., Tada Y., Ma G., Li Q., Suzuki N., Shimada H., Ochiai T.
  • Journal Title: Front.Biosci. 13 Pages: 2578-2587
• [Journal Article] Retrovirus-mediated transduction of a short hairpin RNA gene for GRP78 fails to downregulate GRP78 expression but leads to cisplatin sensitization in HeLa cells
  • Author(s): Suzuki T., Lu J., Hu G., Kita K., Suzuki N.
  • Journal Title: Oncology Reports (in press)
• [Presentation] カドミウムによるヒト細胞増殖阻害に関わるユビキチン様タンパクSUMO-2/3 (2010)
  • Author(s): 菅谷茂、古川聡子、喜多和子、田中健史、紀仲秋、鈴木信夫
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド(神戸市中央区)
  • Year and Date: 20101207-20101210
• [Presentation] 細胞外に添加されたHSPB-Aによるコケイン症候群患者由来細胞の紫外線致死抵抗化 (2010)
  • Author(s): 喜多和子、杉田克生、陳仕萍、佐藤哲生、郭文智、鈴木敏和、菅谷茂、鈴木信夫
  • Organizer: 日本放射線影響学会第53回大会
  • Place of Presentation: 京都
  • Year and Date: 20101020-20101022
• [Presentation] ユビキチン様タンパクSUMO-2/3のヒト細胞で見られるX線照射後のDNA合成能の増大に関わる役割 (2009)
  • Author(s): 郭文智、菅谷茂、佐藤守、朝長毅、野村文夫、鈴木信夫
  • Organizer: 日本放射線影響学会第51回大会
  • Place of Presentation: 北九州(小倉)
  • Year and Date: 20091118-20091121
• [Presentation] ヒト細胞の紫外線致死抵抗化に関わるタンパク質annexin IIとその代謝を調節するHSP27 (2009)
  • Author(s): 陳仕萍、喜多和子、金元虎、〓暁波、菅谷茂、鈴木敏和、鈴木信夫
  • Organizer: 日本放射線影響学会第52回大会
  • Place of Presentation: 広島
  • Year and Date: 20091111-20091113
• [Presentation] ユビキチン様タンパクSUMO-2を介したX線照射後のDNA合成能の増大に関わる腫瘍転移抑制因子NM23-H1の発見 (2009)
  • Author(s): 菅谷茂、郭文智、佐藤守、朝長毅、野村文夫、日和佐隆樹、瀧口正樹、喜多和子、鈴木信夫
  • Organizer: 日本放射線影響学会第52回大会
  • Place of Presentation: 広島
  • Year and Date: 20091111-20091113
• [Presentation] Screening of SMG7-binding peptides by combination of phage display and docking simulation analysis (2008)
  • Author(s): Zahed M., Suzuki T., Tamura Y., Suzuki N.
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] Type 5 adenoviruses bearing the type 35-derived fiber-knob region enhanced the infectivity and increased the anti-tumor effects to CAR-low expressing tumors (2008)
  • Author(s): Tagawa M., Kawamura K., Ma G., Li Q., Takei Y., Suzuki N., Yamaguchi N., Tada Y., Hiroshima K. Shimada H.
  • Organizer: 16th Annual Congress of the European Society of Gene and Cell Therapy
  • Place of Presentation: Brugge, Belgium
  • Year and Date: 2008-11-15
• [Presentation] IFN-lambda induces growth suppression of human esophageal carcinoma cells and enhances the sensitivity to chemotherapy (2008)
  • Author(s): Li Q., Kawamura K., Ma G., Suzuki N., Takei Y., Yamaguchi N., Iwata F., Numasaki M., Shimada H., Tagawa M.
  • Organizer: 16th Annual Congress of the European Society of Gene and Cell Therapy
  • Place of Presentation: Brugge, Belgium
  • Year and Date: 2008-11-14
• [Presentation] E1B 55kDa欠損アデノウイルスと抗がん剤併用による食道がんに対する細胞傷害活 Anti-tumor effects produced by combination of E1B-55kDa-deleted Ad and chemotherapeutic agents for esophageal carcinoma (2008)
  • Author(s): 馬光宇、川村希代子、李全海、武井優輝,山口直人,鈴木信夫、島田英昭、田川雅敏
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-28
• [Presentation] Combinatory use of adenoviruses-mediated cell death, chemotherapeutuc agents and a novel interferon produced enhanced cytotoxicity to targeted tumor cells (2008)
  • Author(s): Tagawa M., Kawamura K., Li Q., Ma G., Ueyama T., Takei Y., Tada Y., Takiguchi Y., Tatsumi K., Suzuki N., Yamaguchi N., Hiroshima K., Shimada H.
  • Organizer: International Society for Cell and Gene Therapy of Cancer 2008
  • Place of Presentation: Shijarzhuang, China
  • Year and Date: 2008-09-21
• [Presentation] Interferon-lambdas inhibit proliferation of human Tumor cells and enhance the sensitivity to chemotherapy (2008)
  • Author(s): Tagawa M., Li Q., Kawamura K., Ma G., Iwata F., Numazaki M., Suzuki N., Shimada H.
  • Organizer: 14th annual meeting of Japan Society of Gene Therapy
  • Place of Presentation: 札幌
  • Year and Date: 2008-06-12
• [Presentation] Replaced fiber-knob region enhanced infectivity of type 5 adenoviruses and increased the anti-tumor effects to CAR-low expressing tumors (2008)
  • Author(s): Tagawa M., Kawamura K., Ma G., Li Q., Ueyama T., Nakamura M., Tada Y., Hiroshima K., Suzuki N., Shimada H.
  • Organizer: 11th annual meeting of American Society of Gene Therapy
  • Place of Presentation: Boston
  • Year and Date: 2008-05-29
• [Presentation] Anti-tumor effects produced by the combinatory use of E1B-55 kDa-deleted adenoviruses and chemotherapeutic agents for human esophageal carcinoma cells (2008)
  • Author(s): Ma G., Kawamura K., Li Q., Ueyama T., Nakamura M., Tada Y., Hiroshima K., Suzuki N., Shimada H., Tagawa M.
  • Organizer: 11th annual meeting of American Society of Gene Therapy
  • Place of Presentation: Boston
  • Year and Date: 2008-05-29
• [Book] 水から学ぶ健康の泉-その3 (2010)
  • Author(s): 鈴木信夫、喜多和子、長谷川博之、吉田政高
  • Total Pages: 68
  • Publisher: 雄文社
• [Book] 水から学ぶ健康の泉-その2 (2008)
  • Author(s): 鈴木信夫、喜多和子
  • Total Pages: 58
  • Publisher: 雄文社
• [Patent(Industrial Property Rights)] 紫外線防止剤 (2008)
  • Inventor(s): 鈴木信夫、戸村真澄、伊藤晴夫、菅谷茂、喜多和子、呂颯
  • Industrial Property Rights Holder: 国立大学法人千葉大学、株式会社I&C、伊藤晴夫
  • Industrial Property Number: 特願,2008-327931
  • Acquisition Date: 2008-12-24