2010 Fiscal Year Final Research Report
Immunofluorescence imaging analysis for intra- and inter-cellular signal transduction mechanisms in chemical carcinogenesis models
| Project/Area Number |
20510071
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | National Cancer Center Research Institute and Research Center for Innovative Oncology, National Cancer Center Hospital East |
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Principal Investigator |
IMAI Toshio National Cancer Center Research Institute and Research Center for Innovative Oncology, National Cancer Center Hospital East, 研究所, 支援施設長 (20342884)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 化学発がん / ラット / シグナル伝達 / イメージング解析 / 多重蛍光染色 |
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| Research Abstract |
Carcinoma tissues obtained from chemically induced rat colorectal and mammary carcinogenesis models were analyzed by a multiple fluorescence method. Frozen tissue sections were stained for beta-catenin and bcl-1 in a colorectal model and estrogen receptor (ER) alpha and leptin receptor (ObR) in a mammary model. Analyses for their co-localization and luminance were performed, and the results might indicate existence of negative feedback systems between beta-catenin and bcl-1 and strong association between ER alpha and ObR in each model.
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