2010 Fiscal Year Final Research Report
Molecular breeding of transgenic tree for phytoremediation of aromatic compounds
| Project/Area Number |
20510076
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental technology/Environmental materials
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| Research Institution | Mie University |
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Principal Investigator |
KIMURA Tetsuya Mie University, 大学院・生物資源学研究科, 准教授 (00281080)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 白色不朽菌 / マンガンペルオキシダーゼ / ヒラタケ / シロイヌナズナ / ビスフェノールA / ポプラ / 塩化安息香酸 |
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| Research Abstract |
Many white-rod fungi produce many lignin peroxidases. Among them, Mnp2 of Pleurotus ostreatus was reported to degrade recalcitrant polyaromatic compounds because of its strong peroxidase activity. We introduced a fusion gene encoding poplar cellulase signal sequence and mnp2 mature region under the control of leaf specific or root specific promoter into Arabidopsis thaliana. Several transgenic plants showed the ability to degrade BisphenolA. This suggests the possibility of molecular breeding of transgenic tree which can express the mnp2 gene. We also introduced a chlorocatechol dioxigenase gene of a soil bacterium Ralstonia eutropha NH9 under the control of CaMV35S promoter into poplar plant (hybrid aspen) and this gene was successfully expressed in the transgenic poplar plant.
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