2010 Fiscal Year Final Research Report
Development of tailor-made catalyst (catalytic antibody) by heterologous immunization
| Project/Area Number |
20550155
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Chemistry related to living body
|
|---|
| Research Institution | Osaka Prefecture University |
|---|
Principal Investigator |
TSUMURAYA Takeshi Osaka Prefecture University, 理学系研究科, 准教授 (00372855)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
FUJII Ikuo 大阪府立大学, 理学系研究科, 教授 (70189984)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Keywords | 酵素 / モノクローナル抗体 / 抗体酵素 / 免疫学 / タンパク質 / 生体分子 |
|---|
| Research Abstract |
In this study we have studied the improvement of the catalytic antibodies by newly devised heterologous immunization. We have developed a method to improve the molecular recognition of the catalytic antibody in antigen-combining site. In heterologous immunization, in which acetyl-transition-state analog was injected into mice after injection of trifluoroacetyl-transition-state analog, catalytic antibodies with higher hydrolytic activities were obtained. The kinetic parameters and amino acid sequences were determined to discuss the catalytic mechanism of the catalytic antibodies.
|
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] Critical Contribution of Aromatic Rings to Specific Recognition of Polyether Rings by Antibody : The Case of Ciguatoxin CTX3C and Its Specific Antibody 1C92008
Author(s)
K. Tsumoto, A. Yokota, Y. Tanaka, M. Ui, T. Tsumuraya, I. Fujii, I. Kumagai, Y. Nagumo, H. Oguri, M. Inoue, M. Hirama
-
Journal Title
J.Mol.Biol. 283
Pages: 12259-12266
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
