ショウジョウバエの精子形成過程における細胞の増殖と成長を制御する機構に関する研究

2009 Fiscal Year Annual Research Report

• Project/Area Number: 20570003
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: 井上 喜博 Kyoto Institute of Technology, 昆虫バイオメディカル教育研究センター, 准教授 (90201938)
• Co-Investigator(Kenkyū-buntansha): 山口 政光 京都工芸繊維大学, 工芸科学研究科, 教授 (00182460)
• Keywords: ショウジョウバエ / 精子形成 / 生殖幹細胞 / インスリン / 細胞周期

Research Abstract

生殖細胞形成過程には、減数分裂前に通常の体細胞とは異なる細胞の増殖、成長がプログラムされている。これら生殖系列の細胞増殖と成長を制御する機構については不明な点が多い。本研究では、ショウジョウバエの精子幹細胞の分裂を誘導する分子機構、精母細胞の成長を制御する機構について解析を進めている。今年度は、とくに精母細胞の成長機構に重点をおいた。減数分裂開始前の成長期において精母細胞はその容積を最大25倍増加させる。これはショウジョウバエの2倍体細胞においては最大の細胞成長である。これを誘導する分子機構はわかっていない。InR突然変異体(InR^<E19>/InR^<339>)では、細胞サイズが最大になるS6期の精母細胞の直径が野生型の68%しかない。I1p2産生細胞を欠く変異体でも野生型の83%に減少していた。この結果は精母細胞の細胞成長もインスリンシグナル伝達系で誘導されていることを示唆している。体細胞では栄養状態が悪く、インスリンが誘導されない場合は転写因子Foxoが翻訳因子e1F4を抑制することにより、代謝を負に制御する。そこでInRとFoxoの2重変異体を作製し、精子幹細胞の分裂効率と精母細胞の成長を観察した。前者には有意な有意な抑圧がみられたが、後者にはそれがみられなかった。したがって、Foxoは精子幹細胞の分裂には負の調節因子として働いているが、精母細胞の成長には関与しない可能性が考えられる。InRの下流にはFoxoに依存しないRAS-MAPK経路も活性化しているので、細胞の種類によりシグナル伝達経路が異なる可能性も考えられる。また精母細胞の成長はInR変異により顕著に抑制されたわけではないので、他の経路の関与も示唆される。現在、Torシグナル伝達経路の構成因子であるTor、Tsc1、Tsc2、Rhebの突然変異、ノックダウン個体により細胞成長が影響を受けるかについて検討中である。

Research Products

• [Journal Article] Syntrophin-2 is required for eye development in Drosophila (2010)
  • Author(s): Nagai, R., Hashimoto, R., Tanaka, Y., Taguchi, O., Sato, M., Matsukage, A., Yamaguchi, M.
  • Journal Title: Exp.Cell Res. 316 Pages: 272-285
  • Peer Reviewed
• [Journal Article] Role of DREF in transcriptional regulation of the Drosophila p53 gene (2010)
  • Author(s): Tue, N.T., Thao, D.T.P., Yamaguchi, M
  • Journal Title: Oncogene 29 Pages: 2060-2069
  • Peer Reviewed
• [Journal Article] Two chitinase-like proteins abundantly accumulated in latex of mulberry show insecticidal activity (2010)
  • Author(s): Kitajima, S., Kamei, K., Taketani, S., Yamaguchi, M., Kawai, F., Komatsu, A., Inukai, Y
  • Journal Title: BMC Biochemistry (In press)
  • Peer Reviewed
• [Journal Article] Regulation of the Drosophila p38b gene by the transcription factor DREF in adult midgut (2010)
  • Author(s): Park, J.-S., Kim, Y.-S., Kim, J.-G., Lee, S.-H., Park, S.-Y., Yamaguchi, M., Yoo, M.-A
  • Journal Title: Biochim.Biophys.Acta. (In press)
  • Peer Reviewed
• [Journal Article] Drosophila Larp associates with poly (A)-binding protein and is required for male fertility and syncytial embryo development (2009)
  • Author(s): Blagden, S., Gatt, M., Ichihara, K., Archambault, K, Lilley, S., Inoue, Y.H., Glover, D.M.
  • Journal Title: Dev Biol 334 Pages: 186-197
  • Peer Reviewed
• [Journal Article] Genetic screening for modifiers of the DREF pathway in Drosophila melanogaster : Identification and characterization of HP6 as a novel target of DREF (2009)
  • Author(s): Ida, H., Suzusho, N., Suyari, O., Yoshida, H., Ohno, K., Hirose, F., Itoh, M., Yamaguchi, M.
  • Journal Title: Nucleic Acids Res. 37 Pages: 1423-1437
  • Peer Reviewed
• [Journal Article] Identification of the Drosophila Mes4 gene as a novel target of the transcription factor DREF (2009)
  • Author(s): Suyari, O., Ida, H., Yoshioka, Y., Kato, Y., Hashimoto, R., Yamaguchi, M.
  • Journal Title: Exp.Cell Res. 315 Pages: 1403-1414
  • Peer Reviewed
• [Journal Article] A novel Drosophila Girdin-like protein is involved in Akt pathway control of cell size (2009)
  • Author(s): Puseenam A., Yoshioka Y., Nagai, R., Hashimoto R., Suyari, O., Itoh, M., Enomoto, A., Takahashi, M., Yamaguchi, M.
  • Journal Title: Exp.Cell Res. 315 Pages: 3370-3380
  • Peer Reviewed
• [Journal Article] Pretaporter, a Drosophila protein serving as a ligand for Draper in the phagocytosis of apoptotic cells (2009)
  • Author(s): Kuraishi, T., Nakagawa, Y., Nagaosa, K., Hashimoto, Y., Ishimoto, T., Moki, T., Fujita, Y., Nakayama, H., Dohmae, N., Shiratsuchi, A., Yamamoto, N., Ueda, K., Yamaguchi, M., Awasaki, T., Nakanishi, Y
  • Journal Title: EMBO J. 28 Pages: 3868-3878
  • Peer Reviewed
• [Presentation] COPI complex that mediates membrane trafficking is required for chromosome segregation and cytokinesis in Drosophila male meiosis (2010)
  • Author(s): 井上喜博
  • Organizer: Memorial Symposium for foundation of Insect Biomedical Research Center
  • Place of Presentation: Kyoto Institute of Technology
  • Year and Date: 2010-03-26
• [Presentation] In vivo roles of components of Dystrophin Glycoprotein Complex in Drosophila (2010)
  • Author(s): 山口政光
  • Organizer: Memorial Symposium for foundation of Insect Biomedical Research Center
  • Place of Presentation: Kyoto Institute of Technology
  • Year and Date: 2010-03-26
• [Presentation] Genetic interaction between Drosophila DNA polymerase a and histone methyltransferase PrSet7 genes (2009)
  • Author(s): Sahashi R., Suyari O., Komai S., Cotterill S., Yamaguchi M.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-12
• [Presentation] ショウジョウバエの胚発生におけるG9あの機能及びヒストン修飾の解析 (2009)
  • Author(s): Konishi T., Ushijima Y., Kato Y., Kimura H., Yamaguchi M.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-12
• [Presentation] Functional analysis of the Drosophila DNA polymerase e p58 subunit (2009)
  • Author(s): Matsuda R., Suyari O., Okumura M., Taguchi O., Yamaguchi M.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-11
• [Presentation] Functional analysis of Mcm10 in Drosophila (2009)
  • Author(s): Inaba Y., Suyari O., Fukui S., Yamaguchi M.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-11
• [Presentation] Transcription factor dNF-Y is involved in differentiation of R7 photoreceptor by regulation of sevenless gene transcription (2009)
  • Author(s): Yoshioka Y., Yamaguchi M.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-11
• [Presentation] Transcription factor NF-Y is involved in regulation of the Hedgehog signaling pathway (2009)
  • Author(s): Hirayabu T., Yoshioka Y., Yamaguchi M.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-11
• [Presentation] Roles of Drosophila transcription factor DREF in bristle development (2009)
  • Author(s): Kawamori A., Yamaguchi M.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-11
• [Presentation] ショウジョウバエの減数分裂細胞におけるOrbitタンパクの細胞内局在とそれに必要な機能ドメインの同定 (2009)
  • Author(s): 宮内千恵, 北澤大姉, 安藤至, 井上喜博
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-10
• [Presentation] The COPI complex is required for spindle formation and cytokinesis during Drosophila meiotic cell divisions (2009)
  • Author(s): 北澤大姉, 井上喜博
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-10
• [Presentation] ショウジョウバエの精子形成過程におけるG9aの機能及びヒストン修飾の解析 (2009)
  • Author(s): 牛島悠太, 小西貴大, 北澤大志, 木村宏, 井上喜博, 山口政光
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-10
• [Presentation] The relevant expression of Drosophila heme oxygenase is necessary for the normal development (2009)
  • Author(s): Taketani, S., Suyari O.C.L., Yamamguchi M.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-10
• [Presentation] Genetic screen for Drosophila myeloid leukemia factor (dMLF) interactants, and analysis of dMLF in JNK pathway (2009)
  • Author(s): Yanai H., Nakao Y., Yoshioka Y., Yamaguchi M.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-10
• [Presentation] Transcriptional regulation of the Hippo pathway-related genes by Drosophila transcription factor DREF (2009)
  • Author(s): Fujiwara S., Ida H., Yamaguchi M.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-09
• [Presentation] DREF, A novel transcriptional regulatory factor of the Drosophila p53 gene (2009)
  • Author(s): Trong-Tue N., Thao D.T.P., Yamaguchi Masamitsu
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-09
• [Presentation] Functional analysis of novel Drosophila Dock family CG31048 (2009)
  • Author(s): Eguchi K., Yoshioka Y., Hiai H., Yamaguchi M.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-09
• [Presentation] In vivo function of Drosophila Syntrophin, a component of the DGC (2009)
  • Author(s): Nagai R., Hashimoto R., Tanaka Y., Sato M., Matsukage A., Yamaguchi M.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-09
• [Presentation] Male germline stem cell division and spermatocyte growth require insul in signaling in Drosophila (2009)
  • Author(s): 井上喜博
  • Organizer: 2009 Recent Bio-research Works
  • Place of Presentation: Pusan National University, Korea
  • Year and Date: 2009-09-11
• [Presentation] Mapping of functional domains in the Orbit protein required for cellular localization in Drosophila male meiosis (2009)
  • Author(s): Miyauchi C., Kitazawa D., Ando I., Inoue Y.H.
  • Organizer: 2009 Recent Bio-research Works
  • Place of Presentation: Pusan National University, Korea
  • Year and Date: 2009-09-11
• [Presentation] AlphaCOP subunit of the COPI complex is required for chromosome segregation and cytokinesis during male meiotic divisions in Drosophila (2009)
  • Author(s): 北澤大志, 井上喜博
  • Organizer: 2009 Recent Bio-research Works
  • Place of Presentation: Pusan National University, Korea
  • Year and Date: 2009-09-11
• [Presentation] Male germline stem cell division and spermatocyte growth require insulin signaling in Drosophila (2009)
  • Author(s): Ueishi S., Shimizu H., Inoue Y.H.
  • Organizer: 2009 Recent Bio-research Works
  • Place of Presentation: Pusan National University, Korea
  • Year and Date: 2009-09-11
• [Presentation] Studies on Drosophila histone methylytransferase G9a in vivo (2009)
  • Author(s): 山口政光
  • Organizer: 2009 Recent Bio-research Works
  • Place of Presentation: Pusan National University, Korea
  • Year and Date: 2009-09-11
• [Presentation] Functional analysis of Drosophila a- and g-Sarcoglycan (2009)
  • Author(s): Ito T., Hashimoto R., Ogata K., Yamaguchi M.
  • Organizer: 2009 Recent Bio-research Works
  • Place of Presentation: Pusan National University, Korea
  • Year and Date: 2009-09-11
• [Presentation] Epigenetic modification contributes to age-related changes in intestinal stem cell differentiation in Drosophila (2009)
  • Author(s): Kim J-G., Lee S-H., Kim Y-S., Yamaguchi M., Yoo M-A.
  • Organizer: 2009 Recent Bio-research Works
  • Place of Presentation: Pusan National University, Korea
  • Year and Date: 2009-09-11
• [Presentation] Role of DREF in transcriptional regulation of the Drosophila p53 gene (2009)
  • Author(s): Trong-Tue N., Thao D.T.P., Yamaguchi M.
  • Organizer: 2009 Recent Bio-research Works
  • Place of Presentation: Pusan National University, Korea
  • Year and Date: 2009-09-11
• [Presentation] Transcriptional regulation of the Drosophila p38b MAPK gene by the DRE/DREF system in adult midgut (2009)
  • Author(s): Park J-S., Kim Y-S., Kim J-G., Park S-Y., Yamaguchi M., Yoo M-A.
  • Organizer: 2009 Recent Bio-research Works
  • Place of Presentation: Pusan National University, Korea
  • Year and Date: 2009-09-11
• [Presentation] Male germline stem cell division and spermatocyte growth require insulin signaling in Drosophila (2009)
  • Author(s): Ueishi S., Shimizu H., Inoue Y.H.
  • Organizer: The 9^<th> Japanese Drosophila Research Conference
  • Place of Presentation: Yamaha Resort Tsumagoi
  • Year and Date: 2009-07-06
• [Presentation] Mapping of functional domains in the Orbit protein required for cellular localization in Drosophila male meiosis (2009)
  • Author(s): Miyauchi C., Kitazawa D., Ando I., Inoue Y.H.
  • Organizer: The 9^<th> Japanese Drosophila Research Conference
  • Place of Presentation: Yamaha Resort Tsumagoi
  • Year and Date: 2009-07-06
• [Presentation] AlphaCOP subunit of the COPI complex is required for chromosome segregation and cytokinesis during male meiotic divisions in Drosophila (2009)
  • Author(s): 北澤大志, 井上喜博
  • Organizer: The 9^<th> Japanese Drosophila Research Conference
  • Place of Presentation: Yamaha Resort Tsumagoi
  • Year and Date: 2009-07-06
• [Presentation] AlphaCOP subunit of the COPI complex is required for chromosome segregation and cytokinesis during male meiotic divisions in Drosophila (2009)
  • Author(s): 北澤大志, 井上喜博
  • Organizer: 日本分子生物学会第9回春期シンポジウム
  • Place of Presentation: 宮崎シーガイア ワールドコンベンションセンター
  • Year and Date: 2009-05-11
• [Book] 虫たちが語る生物学の未来 (2009)
  • Author(s): 井上喜博
  • Total Pages: 227
  • Publisher: 財団法人・衣笠会