Genetic studies of cell proliferation and growth in Drosophila spermatogenesis

2010 Fiscal Year Final Research Report

• Project/Area Number: 20570003
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: INOUE Yoshihiro Kyoto Institute of Technology, 昆虫バイオメディカル教育研究センター, 准教授 (90201938)
• Co-Investigator(Kenkyū-buntansha): YAMAGUCHI Masamitsu 京都工芸繊維大学, 工芸科学研究科, 教授 (00182460)
• Project Period (FY): 2008 – 2010
• Keywords: ショウジョウバエ / 精子形成 / 生殖幹細胞 / インスリン / 細胞周期

Research Abstract

It has recently been reported that some aspects of cell division and cell growth in germ line cells are different from those in somatic cells. Our genetic analysis of insulin-like peptides, insulin receptor and its signal transducers revealed that the insulin signal induces both division of male germline stem cells and growth of premeiotic spermatocytes. On the other hand, FOXO transcription factor shown as a target of the insulin signaling in somatic cells is not involved in the cell growth but in the germline stem cell division. TOR signaling pathway is also required for the remarkable cell growth of spermatocytes.

Research Products

• [Journal Article] A Drosophila model for the screening of bioavailable NADPH oxidase inhibitors and antioxidants. (2011)
  • Author(s): Tu Anh, N.T., Nishitani, M., Harada, S., Yamaguchi, M., Kamei, K.
  • Journal Title: Mol.Cell.Biochem. In press
  • Peer Reviewed
• [Journal Article] Regulation of the Drosophila p38b gene by the transcription factor DREF in adult midgut. (2010)
  • Author(s): Park, J.-S., Kim, Y.-S., Kim, J.-G., Lee, S.-H., Park, S.-Y., Yamaguchi, M., Yoo, M.-A
  • Journal Title: Biochim. Biophys. Acta. 1799 Pages: 510-519
  • Peer Reviewed
• [Journal Article] Syntrophin-2 is required for eye development in Drosophila. (2010)
  • Author(s): Nagai, R., Hashimoto, R., Tanaka, Y., Taguchi, O., Sato, M., Matsukage, A., Yamaguchi, M.
  • Journal Title: Exp.Cell Res. 316 Pages: 272-285
  • Peer Reviewed
• [Journal Article] Over-expression of transglutaminase in the Drosophila eye imaginal disc induces a rough eye phenotype. (2010)
  • Author(s): Umehara, M., Ichikawa, A., Sakamoto, H., Yamada, A., Yoshioka, Y., Yamaguchi, M., Ikura, K.
  • Journal Title: Mol.Cell.Biochem 342 Pages: 223-232
  • Peer Reviewed
• [Journal Article] Drosophila Syntrophins are involved in locomotion and regulation of synaptic morphology. (2010)
  • Author(s): Nagai, R., Hashimoto, R., Yamaguchi, M.
  • Journal Title: Exp.Cell Res 316 Pages: 2313-2321
  • Peer Reviewed
• [Journal Article] Overexpression of transglutaminase in the Drosophila wing disc induced an extra wing crossvein phenotype. (2010)
  • Author(s): Ichikawa, A., Yamada, A., Sakamoto, H., Umehara, M., Yoshioka, Y., Yamaguchi, M., Ikura, K.
  • Journal Title: Biosci. Biotechnol. Biochem. 74 Pages: 2494-2496
  • Peer Reviewed
• [Journal Article] NF-Y transcriptionally regulates the Drosophila p53 gene. (2010)
  • Author(s): Tue, N.T., Yoshioka, Y., Yamaguchi, M.
  • Journal Title: Gene 47 Pages: 1-7
  • Peer Reviewed
• [Journal Article] dGirdin a new player of Akt/PKB signaling in Drosophila melanogaster. (2010)
  • Author(s): Yamaguchi, M., Suyari, O., Nagai, R., Takahashi, M.
  • Journal Title: Front.Biosci. 15 Pages: 1164-1171
  • Peer Reviewed
• [Journal Article] Genetic screening for modifiers of the DREF pathway in Drosophila melanogaster : Identification and characterization of HP6 as a novel target of DREF. (2009)
  • Author(s): Ida, H., Suzusho, N., Suyari, O., Yoshida, H., Ohno, K., Hirose, F., Itoh, M., Yamaguchi, M.
  • Journal Title: Nucleic Acids Res. 37 Pages: 1423-1437
  • Peer Reviewed
• [Journal Article] Identification of the Drosophila Mes4 gene as a novel target of the transcription factor DREF. (2009)
  • Author(s): Suyari, O., Ida, H., Yoshioka, Y., Kato, Y., Hashimoto, R., Yamaguchi, M.
  • Journal Title: Exp.Cell Res. Pages: 1403-1437
  • Peer Reviewed
• [Journal Article] Drosophila Larp associates with poly(A)-binding protein and is required for male fertility and syncytial embryo development. (2009)
  • Author(s): Blagden, S., Gatt, M., Ichihara, K., Archambault, K., Lilley, S., Inoue, Y.H., Glover, D.M.
  • Journal Title: Dev Biol 334 Pages: 186-197
  • Peer Reviewed
• [Journal Article] A novel Drosophila Girdin-like protein is involved in Akt pathway control of cell size. (2009)
  • Author(s): Puseenam A., Yoshioka Y., Nagai, R., Hashimoto R., Suyari, O., Itoh, M., Enomoto, A., Takahashi, M., Yamaguchi, M.
  • Journal Title: Exp.Cell Res. 315 Pages: 3370-3380
  • Peer Reviewed
• [Journal Article] Pretaporter, a Drosophila protein serving as a ligand for Draper in the phagocytosis of apoptotic cells. (2009)
  • Author(s): Kuraishi, T., Nakagawa, Y., Nagaosa, K., Hashimoto, Y., Ishimoto, T., Moki, T., Fujita, Y., Nakayama, H., Dohmae, N., Shiratsuchi, A., Yamamoto, N., Ueda, K., Yamaguchi, M., Awasaki, T., Nakanishi, Y.
  • Journal Title: EMBO J. 28 Pages: 3868-3878
  • Peer Reviewed
• [Journal Article] Role of DREF in transcriptional regulation of the Drosophila p53 gene. (2009)
  • Author(s): Tue, N.T., Thao, D.T.P., Yamaguchi, M.
  • Journal Title: Oncogene 29 Pages: 2060-2069
  • Peer Reviewed
• [Journal Article] The DRE/DREF transcriptional regulatory system : a master key for cell proliferation. (2008)
  • Author(s): Matsukage, A., Hirose, F., Yoo, Mi-Ae, Yamaguchi, M.
  • Journal Title: Biochim. Biophys. Acta. 1779 Pages: 81-89
  • Peer Reviewed
• [Journal Article] Relevant expression of Drosophila Heme oxygenase is necessary for the normal development of insect tissues. (2008)
  • Author(s): Cui, L., Yoshioka, Y., Suyari, O., Kohno, Y., Zhang, X., Adachi, Y., Ikehara, S., Yoshida, T., Yamaguchi, M., Taketani, S.
  • Journal Title: Biochem. Biophys. Res.Commun. 377 Pages: 1156-1161
  • Peer Reviewed
• [Journal Article] Big brain, a Drosophila homologue of mammalian aquaporin, is regulated by the DRE/DREF system. (2008)
  • Author(s): Park, J.S., Kim, S.R., Park, S.Y., Yang, D.J., Lee, S.H., Choi, Y.J., Bae, M.K., Yamaguchi, M., Kim, Y.S., Yoo, M.A.
  • Journal Title: Biochim. Biophys. Acta. 1779 Pages: 789-796
  • Peer Reviewed
• [Journal Article] Heat shock transcription factor 1(HSF1)-activating compounds suppress polyglutamine-induced neurodegeneration through induction of multiple molecular chaperones. (2008)
  • Author(s): Fujikake, N., Nagai, Y., Popiel, A., Okamoto, Y., Yamaguchi, M., Toda, T.
  • Journal Title: J.Biol.Chem. 283 Pages: 26188-26197
  • Peer Reviewed
• [Journal Article] ERK2 CD domain mutation from human cancer cell line enhanced anchorage-independent cell growth and abnormality in Drosophila. (2008)
  • Author(s): Mahalingam, M., Arvind, R., Ida, H., Murugan, A.K., Yamaguchi, M., Tsuchida, N.
  • Journal Title: Oncology Rep. 20 Pages: 957-962
  • Peer Reviewed
• [Journal Article] Transcriptional regulation of the Drosophila moira and osa genes by the DREF pathway. (2008)
  • Author(s): Nakamura, K., Ida, H., Yamaguchi, M.
  • Journal Title: Nucleic Acids Res. 36 Pages: 3905-3915
  • Peer Reviewed
• [Journal Article] Characterization of Drosophila G9a in vivo and identification of genetic interactants. (2008)
  • Author(s): Kato, Y., Kato, M., Tachibana, M., Shinkai, Y., Yamaguchi, M.
  • Journal Title: Genes Cells 13 Pages: 703-722
  • Peer Reviewed
• [Journal Article] Transcription factor NF-Y is involved in regulation of the JNK pathway during Drosophila thorax development. (2008)
  • Author(s): Yoshioka, Y., Suyari, O., Yamaguchi, M.
  • Journal Title: Genes Cells 13 Pages: 117-130
  • Peer Reviewed
• [Journal Article] Drosophila Myc is required for normal DREF gene expression. (2008)
  • Author(s): Thao, D.T.P., Seto, H., Yamaguchi, M.
  • Journal Title: Exp.Cell Res. 314 Pages: 184-192
  • Peer Reviewed
• [Journal Article] The myeloid leukemia factor interacts with COP9 signalosome subunit 3 in Drosophila melanogaster. (2008)
  • Author(s): Sugano, W., Ohno, K., Yoneda-Kato, N., Kato, J., Yamaguchi, M.
  • Journal Title: FEBS J. 275 Pages: 588-600
  • Peer Reviewed
• [Presentation] Genetic interaction between LARP family proteins and multiple roles of LARP1 in meiotic divisions in Drosophila males. (2010)
  • Author(s): Inoue Y.H., Ichihara, K.
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸国際会議場、兵庫県神戸市
  • Year and Date: 2010-12-12
• [Presentation] Dynamic localization of the Orbit/CLASP during meiotic divisions in Drosophila males and elucidation of its regulatory mechanism. (2010)
  • Author(s): Miyauchi, C., Ando, I., Kitazawa D., Inoue Y.H.
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸国際会議場、兵庫県神戸市
  • Year and Date: 2010-12-12
• [Presentation] インスリンならびにTORシグナル伝達系によるショウジョウバエ精母細胞の細胞成長の誘導 (2010)
  • Author(s): 緒方翼、上石暁、井上喜博
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸国際会議場、兵庫県神戸市
  • Year and Date: 2010-12-12
• [Presentation] Orbit/CLASP, a possible direct linker between central spindle and contractile ring in Drosophila male meiosis. (2010)
  • Author(s): Kitazawa D., Miyauchi, C., Inoue Y.H.
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸国際会議場、兵庫県神戸市
  • Year and Date: 2010-12-12
• [Presentation] ショウジョウバエの精子形成過程におけるG9aの機能及びヒストン修飾の解析 (2010)
  • Author(s): 牛島悠太、北澤大志、井上喜博、木村宏、山口政光
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸国際会議場、兵庫県神戸市
  • Year and Date: 2010-12-11
• [Presentation] Novel RNA binding protein families, LARPs are required for male meiotic divisions in Drosophila and show an interaction with Polycomb group proteins. (2010)
  • Author(s): Inoue Y.H., Ichihara K., Kitazawa D., M.Yamaguchi
  • Organizer: International symposium on "Epigenome network, development and reprogramming of germ cells"
  • Place of Presentation: Centinental Hall, Fukuoka, Japan
  • Year and Date: 2010-11-22
• [Presentation] A loss of LARP1 results in multiple phenotypes of spermatogenesis in Drosophila. (2010)
  • Author(s): Ichihara, K., Shimizu, H., Inoue Y.H.
  • Organizer: 1st Ann.Meeting of the LARP society. 1st International meeting of the LARP Society
  • Place of Presentation: Imperial College, UK
  • Year and Date: 2010-09-02
• [Presentation] The COPI complex is required for spindle formation and cytokinesis during meiotic cell division in Drosophila. (2010)
  • Author(s): 北澤大志, 井上喜博
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪国際会議場,大阪市
  • Year and Date: 2010-05-19
• [Presentation] Male germline stem cell division and spermatocyte growth require insulin signaling in Drosophila. (2009)
  • Author(s): 井上喜博
  • Organizer: 2009 Recent Bio-research Works.
  • Place of Presentation: Pusan National University, Korea
  • Year and Date: 2009-09-11
• [Presentation] AlphaCOP subunit of the COPI complex is required for chromosome segregation and cytokinesis during male meiotic divisions in Drosophila. (2009)
  • Author(s): 北澤大志, 井上喜博
  • Organizer: 2009 Recent Bio-research Works.
  • Place of Presentation: Pusan National University, Korea
  • Year and Date: 2009-09-11
• [Presentation] Studies on Drosophila histone methylytransferase G9a in vivo. (2009)
  • Author(s): 山口政光
  • Organizer: 2009 Recent Bio-research Works.
  • Place of Presentation: Pusan National University, Korea
  • Year and Date: 2009-09-11
• [Presentation] Male germline stem cell division and spermatocyte growth require insulin signaling in Drosophila. (2009)
  • Author(s): Ueishi S., Shimizu H., Inoue Y.H.
  • Organizer: The 9th Japanese Drosophila Research Conference.
  • Place of Presentation: Yamaha Resort Tsumagoi
  • Year and Date: 2009-07-06
• [Presentation] Mapping of functional domains in the Orbit protein required for cellular localization in Drosophila male meiosis. (2009)
  • Author(s): Miyauchi C., Kitazawa D., Ando I., Inoue Y.H.
  • Organizer: The 9th Japanese Drosophila Research Conference.
  • Place of Presentation: Yamaha Resort Tsumagoi
  • Year and Date: 2009-07-06
• [Presentation] AlphaCOP subunit of the COPI complex is required for chromosome segregation and cytokinesis during male meiotic divisions in Drosophila. (2009)
  • Author(s): 北澤大志、井上喜博
  • Organizer: 日本分子生物学会第9回春期シンポジウム
  • Place of Presentation: 宮崎シーガイア ワールドコンベンションセンター
  • Year and Date: 2009-05-11
• [Remarks] ホームページ等
  • URL: http://sites.google.com/site/ibmrcdrosophila/akusesu-1