Molecular mechanism of genome maintenance in Thermus thermophilus

2010 Fiscal Year Final Research Report

• Project/Area Number: 20570131
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Osaka University
• Principal Investigator: MASUI Ryoji Osaka University, 大学院・理学研究科, 准教授 (40252580)
• Project Period (FY): 2008 – 2010
• Keywords: 酵素 / 核酸 / 生体分子 / ゲノム / 細菌

Research Abstract

I prepared Thermus thermophilus HB8 proteins involved in mismatch repair and base excision repair systems and performed reconstitution of repair reactions, protein-protein interaction analysis and crystal structural analyss. Based on the results, I revealed that novel interactions between mismatch repair proteins contribute to strand discrimination, the nuclease which degrades an error-strand recognizes single-straded DNA by its unique structure, and two DNA polymerases function in different pathways of base excision repair.

Research Products

• [Journal Article] Long type endonuclease IV has an additional C-terminal loop that increases binding affinity to DNA. (2011)
  • Author(s): Asano, R., Ishikawa, H., Nakane, S., Nakagawa, N., Kuramitsu, S., Masui, R.
  • Journal Title: Acta Crystallogr. D67 Pages: 149-155
  • Peer Reviewed
• [Journal Article] A novel single-stranded DNA-specific 3'-5' exonuclease, Thermus thermophilus exonuclease I, is involved in several DNA repair pathways. (2010)
  • Author(s): Shimada, A., Masui, R., Nakagawa, N., Takahata, Y., Kim, K., Kuramitsu, S., Fukui, K.
  • Journal Title: Nucleic Acids Res. 38 Pages: 5692-5705
  • Peer Reviewed
• [Journal Article] Structure of RecJ exonuclease defines its specificity for single-stranded DNA. (2010)
  • Author(s): Wakamatsu, T., Kitamura, Y., Kotera, Y., Nakagawa, N., Kuramitsu, S., Masui, R.
  • Journal Title: J.Biol.Chem. 285 Pages: 9762-9769
  • Peer Reviewed
• [Journal Article] Characterization of DNA polymerase X from Thermus thermophilus HB8 reveals the POLXc and PHP domains are both required for 3'-5' exonuclease activity. (2009)
  • Author(s): Nakane, S., Nakagawa, N., Kuramitsu, S., Masui, R.
  • Journal Title: Nucleic Acids Res. 37 Pages: 2037-2052
  • Peer Reviewed
• [Journal Article] Bound nucleotide controls the endonuclease activity of mismatch repair enzyme MutL. (2008)
  • Author(s): Fukui, K., Nishida, M., Nakagawa, N., Masui, R., Kuramitsu, S.
  • Journal Title: J.Biol.Chem. 283 Pages: 12136-12145
  • Peer Reviewed
• [Presentation] Structural and functional analysis of bacterial DNA polymerase X involved in DNA repair processes. (2010)
  • Author(s): 中根修平, 増井良治
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-09
• [Presentation] DNAミスマッチ修復における鎖除去ステップの解析 (2010)
  • Author(s): 島田敦広, 増井良治
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] Contribution of OB-fold domain to the activity of RecJ exonuclease. (2009)
  • Author(s): 若松泰介, 増井良治
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-10
• [Presentation] DNAミスマッチ修復系初期反応におけるMutLエンドヌクレアーゼ活性の制御機構 (2008)
  • Author(s): 福井健二, 増井良治
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-12
• [Presentation] 好熱菌由来endonuclease IVの3'-5' exonuclease活性発現機構の解析 (2008)
  • Author(s): 淺野瑠一, 増井良治
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-12
• [Book] やさしい原理からはいるタンパク質科学実験法3:タンパク質のはたらきを知る(共著) (2009)
  • Author(s): 倉光成紀, 増井良治
  • Total Pages: 1-21
  • Publisher: 化学同人