2010 Fiscal Year Final Research Report
Single-molecule tracking study of the mechanisms of signal transduction in lipid rafts of cell plasma membranes
| Project/Area Number |
20570154
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Kyoto University |
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Principal Investigator |
SUZUKI KEenichi Kyoto University, 物質ー細胞統合システム拠点, 民間等共同研究員 (50423059)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 1分子観察 / 細胞膜 / ラフト / シグナル伝達 / 受容体 |
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| Research Abstract |
It has turned out that in steady-state cell plasma membranes, GPI-anchored proteins formed transient homo-dimers based on the extracellular protein-protein interactions. In the presence of protein-protein interactions, lipid interactions were found to facilitate the formation of the homo-dimers, while this was not observed in the absence of the protein interactions. Furthermore, it was found that upon ligation of GPI-anchored receptors, very stable homo-oligomers were formed, the oligomers recruited other raft lipid, ganglioside, and induced raft-like structure in the oligomers. The formation of the raft-like structure in the oligomers was essential for the downstream signaling such as intracellular calcium response.
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[Journal Article] Membrane molecules mobile even after chemical fixation.2010
Author(s)
K.A.K.Tanaka^*, K.G.N.Suzuki^* (*equal contribution), Y.M.Shirai, S.T.Shibutani, M.S.Miyahara, H.Tsuboi, M.Yahara, A.Yoshimura, S.Mayor, T.K.Fujiwara, A.Kusumi.
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Journal Title
Nature methods 7
Pages: 865-866
Peer Reviewed
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