2010 Fiscal Year Final Research Report
Elucidation of E.coli 100S ribosome structure and formation mechanisms
| Project/Area Number |
20570167
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Osaka Medical College |
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Principal Investigator |
YOSHIDA Hideji Osaka Medical College, 医学部, 准教授 (60288735)
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| Co-Investigator(Kenkyū-buntansha) |
MAKI Yasushi 大阪医科大学, 医学部, 講師 (60401733)
SAKAI Akiko 大阪医科大学, 医学部, 助教 (30225750)
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| Project Period (FY) |
2008 – 2010
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| Keywords | リボソーム / 大腸菌 / ストレス応答 |
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| Research Abstract |
In order to elucidate the mechanisms of E.coli 100S ribosome formation, the structure of the 100S ribosome was observed by electron cryomicroscopy. The results revealed that the 100S ribosome is formed by two tRNA-free 70S ribosomes through the interactions between their 30S subunits and that the S2, S3 and S5 proteins are likely to be directly involved in the interactions. The functions of these proteins are related to the binding of mRNA to the ribosome. The undesirable binding of mRNA to the 100S ribosomes may be inhibited by the conformational changes around the S2-S5 region.
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