2010 Fiscal Year Final Research Report
Analysis of M phase-regulation by GdX
| Project/Area Number |
20570175
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Tohoku University |
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Principal Investigator |
ISHIDA Noriko Tohoku University, 大学院・医学系研究科, 助教 (10361073)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAYAMA Keiko 東北大学, 大学院・医学系研究科, 教授 (60294972)
FUNAYAMA Ryo 東北大学, 大学院・医学系研究科, 助教 (20452295)
NAKANO Seiji 東北大学, 大学院・医学系研究科, 助手 (00529448)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 翻訳語修飾 / ユビキチン様タンパク質 / 細胞周期 / 細胞分裂 |
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| Research Abstract |
We found that cyclin F was covalently conjugated with GdX (GdXylation). Our data suggested that GdXylation of cyclin F regulated its protein stability and cell cycle progression. Although we identified 11 GdX- and 23 RNF (candidate of GdXligase)-binding proteins by biochemical techniques, identification of E1, E2 and E3 enzymes for GdXylation could not be achieved.It was suggested that RNF is functional as a ubiquitinligase in mouse spermatogenesis by analysis of RNF-deficient mice. So we will develop the functional analysis of RNF in future.
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[Journal Article] Skp2 is required for survivalof aberrantly proliferating Rb1-deficient cells and for tumorigenesis in Rb1(+/-) mice.2010
Author(s)
Wang H, Bauzon F, Ji P, Xu X, Sun D, Locker J, Sellers RS, Nakayama K, Nakayama KI, Cobrinik D, Zhu L.
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Journal Title
Nat.Genet. 42
Pages: 83-88
Peer Reviewed
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[Journal Article] Human synovial sarcoma proto-oncogene Syt is essential for early embryonic development through the regulation of cell migration.2009
Author(s)
Kimura T, Sakai M, Tabu K, Wang L, Tsunematsu R, Tsuda M, Sawa H, Nagashima K, Nishihara H, Hatakeyama S, Nakayama K, Ladanyi M, Tanaka S, Nakayama KI.
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Journal Title
Lab.Invest. 89
Pages: 645-656
Peer Reviewed
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[Journal Article] The mechanoregulation of proliferation.2009
Author(s)
Jiang X, Austin PF, Niederhoff RA, Manson SR, Riehm JJ, Cook BL, Pengue G, Chitaley K, Nakayama K, Nakayama KI, Weintraub SJ.
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Journal Title
Mol.Cell.Biol. 18
Pages: 5104-5114
Peer Reviewed
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[Journal Article] Fbxw7 acts as a critical fail-safe against premature loss of hematopoietic stem cells and development of T-ALL.2008
Author(s)
Matsuoka S, Oike Y, Onoyama I, Iwama A, Arai F, Takubo K, Mashimo Y, Oguro H, Nitta E, Ito K, Miyamoto K, Yoshiwara H, Hosokawa K, Nakamura Y, Gomei Y, Iwasaki H, Hayashi Y, Matsuzaki Y, Nakayama K, Ikeda Y,Hata A, Chiba S, Nakayama KI, Suda T.
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Journal Title
Genes Dev. 22
Pages: 986-991
Peer Reviewed
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[Journal Article] Targeting the p27 E3 ligase SCFSkp2 results in p27- and Skp2-mediated cell cycle arrest, and activation of autophagy.2008
Author(s)
Chen Q, Xie W, Kuhn DJ, Voorhees PM, Lopez-Girona A, Mendy D, Corral LG, Krenitsky VP, Xu W, Moutouh-de Parseval L, Webb DR, Mercurio F, Nakayama KI, Nakayama K. Orlowski RZ.
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Journal Title
Blood 111
Pages: 4690-4699
Peer Reviewed
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