Dynamic functions of chaperonins in the cell

2010 Fiscal Year Final Research Report

• Project/Area Number: 20570182
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Akita University
• Principal Investigator: KUBOTA Hiroshi Akita University, 工学資源学研究科, 教授 (80332724)
• Project Period (FY): 2008 – 2010
• Keywords: 分子シャペロン / CCT / フォールディング / 基質特異性 / サブユニット / タンパク質相互作用 / シャペロニン / HSP60

Research Abstract

CCT and HSP60 are molecular chaperones required for eukaryotic cells including human cells. CCT and HSP60 assist in the folding of proteins in eukaryotic cytosol and mitochondria, respectively. Here, we report that individual CCT subunits play distinctive roles in substrate protein recognition and that HSP60 has an unique ATP-dependent assembly system to produce functional complex.

Research Products

• [Journal Article] Analyzing the aggregation of polyglutamine-expansion proteins and its modulation by molecular chaperones (review). (2011)
  • Author(s): Kubota H., Kitamura A., Nagata K.
  • Journal Title: Methods 53 Pages: 267-274
  • Peer Reviewed
• [Journal Article] Increased expression of co-chaperone HOP with HSP90 and HSC70 and complex formation in human colonic carcinoma. (2010)
  • Author(s): Kubota H., Yamamoto S., Itoh E., Abe Y., Nakamura A., Izumi Y., Okada H., Iida M., Nanjo H., Itoh H., Yamamoto Y.
  • Journal Title: Cell Stress Chaperones 15 Pages: 1003-1011
  • Peer Reviewed
• [Journal Article] Gentamicin inhibits HSP70-assisted protein folding by interfering with substrate recognition. (2010)
  • Author(s): Yamamoto S., Nakano S., Owari K., Fuziwara K., Ogawa N., Otaka M., Tamaki K., Watanabe S., Komatsuda A., Wakui H., Sawada K.I., Kubota H., Itoh H.
  • Journal Title: FEBS Lett. 584 Pages: 645-651
  • Peer Reviewed
• [Journal Article] Solution structure and dynamics of mouse ARMET. (2010)
  • Author(s): Hoseki J., Sasakawa H., Yamaguchi Y., Maeda M., Kubota H., Kato K., Nagata K.
  • Journal Title: FEBS Lett. 584 Pages: 1536-1542
  • Peer Reviewed
• [Journal Article] Amyloid oligomers : dynamics and toxicity in the cytosol and nucleus (review). (2010)
  • Author(s): Kitamura A., Kubota H.
  • Journal Title: FEBS J. 277 Pages: 1369-1379
  • Peer Reviewed
• [Journal Article] Fluorescently labeled proteins as a tool for analyzing the dynamics of protein quality control in living cells. (2010)
  • Author(s): Kubota H., Kitamura A., Itoh H., Kinjo M., Nagata K.
  • Journal Title: Int.J.Soc.Mater.Eng.Resour. 17 Pages: 1-4
  • Peer Reviewed
• [Journal Article] Autophagic elimination of misfolded procollagen aggregates in the endoplasmic reticulum as a means of cell protection. (2009)
  • Author(s): Ishida Y., Yamamoto A., Kitamura A., Lamande S.R., Yoshimori T., Bateman J.F., Kubota H., Nagata K.
  • Journal Title: Mol.Biol.Cell 20 Pages: 2744-2754
  • Peer Reviewed
• [Journal Article] Elastic fiber assembly is disrupted by excessive accumulation of chondroitin sulfate in the human dermal fibrotic disease, keloid. (2009)
  • Author(s): Ikeda M., Naitoh M., Kubota H., Ishiko T., Yoshikawa K., Yamawaki S., Kurokawa M., Utani A., Nakamura T., Nagata K., Suzuki S.
  • Journal Title: Biochem.Biophys.Res.Commun. 390 Pages: 1221-1228
  • Peer Reviewed
• [Journal Article] Guidelines for the nomenclature of the human heat shock proteins (review). (2009)
  • Author(s): Kampinga H.H., Hageman J., Vos M.J., Kubota H., Tanguay R.M., Bruford E.A., Cheetham M.E., Chen B., Hightower L.E.
  • Journal Title: Cell Stress Chaperones 14 Pages: 105-111
  • Peer Reviewed
• [Journal Article] Quality control against misfolded proteins in the cytosol : a network for cell survival (review). (2009)
  • Author(s): Kubota H.
  • Journal Title: J.Biochem. 146 Pages: 609-616
  • Peer Reviewed
• [Journal Article] MKKS is a centrosome-shuttling protein degraded by disease-causing mutations via CHIP-mediated ubiquitination. (2008)
  • Author(s): Hirayama S., Yamazaki Y., Kitamura A., Oda Y., Morito D., Okawa K., Kimura H., Cyr D.M., Kubota H., Nagata K.
  • Journal Title: Mol.Biol.Cell 19 Pages: 899-911
  • Peer Reviewed
• [Journal Article] Cisplatin differently affects amino terminal and carboxyl terminal domains of HSP90. (2008)
  • Author(s): Ishida R., Takaoka Y., Yamamoto S., Miyazaki T., Otaka M., Watanabe S., Komatsuda A., Wakui H., Sawada K., Kubota H., Itoh H.
  • Journal Title: FEBS Lett. 582 Pages: 3879-3883
  • Peer Reviewed
• [Journal Article] High serum concentrations of autoantibodies to HSP47 in nonspecific interstitial pneumonia compared with idiopathic pulmonary fibrosis. (2008)
  • Author(s): Kakugawa T., Yokota S., Mukae H., Kubota H., Sakamoto N., Mizunoe S., Matsuoka Y., Kadota J., Fujii N., Nagata K., Kohno S.
  • Journal Title: BMC Pulm.Med. 8 Pages: 23
  • Peer Reviewed
• [Presentation] 分子シャペロンPrefoldinは変異ハンチンチンのオリゴマーおよび凝集形成を阻害して細胞死を減少させる (2010)
  • Author(s): 田代絵梨佳、武藤秀樹、座古保、ソルヤード・カリン、宮沢誠、北浦廣剛、北村朗、久保田広志、前田瑞夫、金城政孝、有賀寛芳
  • Organizer: 第33回日本分子生物学会年会-第83 回日本生化学会大会-合同大会
  • Place of Presentation: 神戸市(口頭発表およびポスター発表)
  • Year and Date: 20101207-20101210
• [Presentation] 筋萎縮性側索硬化症を引き起こす原因タンパク質の脱凝集過程と細胞毒性の関係性 (2010)
  • Author(s): 北村朗,稲田のりこ,久保田広志,松本弦,永田和宏,金城政孝
  • Organizer: 第33回日本分子生物学会年会-第83回日本生化学会大会-合同大会
  • Place of Presentation: 神戸市(ポスター発表)
  • Year and Date: 20101207-20101210
• [Presentation] 免疫抑制剤MizoribineによるHSP60阻害機構の解明 (2010)
  • Author(s): 田名邉雅子、宮崎敏夫、石田竜一、高橋弘樹、久保田広志、伊藤英晃
  • Organizer: 第5回臨床ストレス応答学会
  • Place of Presentation: 徳島市(口頭発表)
  • Year and Date: 20101119-20101120
• [Presentation] Molecular chaperone prefoldin blocks the formation of mutant Huntingtin oligomer and inclusion body, resulting in reduction of its Cytotoxity. (2010)
  • Author(s): Erika Tashiro, Hideki Muto, Tamotsu Zako, Makoto Miyazawa, Hirotake Kitaura, Akira Kitamura, Hiroshi Kubota, Mizuo Maeda, Masataka Kinjo, Hiroyoshi Ariga
  • Organizer: 40th Annual Meeting of the Society for Neuroscience (Neuroscience 2010)
  • Place of Presentation: San Diego(ポスター発表)
  • Year and Date: 20101113-20101117
• [Presentation] Formation of HOP-containing chaperone complexes and the effect of 17-AAG, an HSP90 inhibitor, on colon cancer cells. (2010)
  • Author(s): Hiroshi Kubota, Soh Yamamoto, Yuzo Yamamoto, Hideaki Itoh
  • Organizer: The 3rd International Symposium on Protein Community
  • Place of Presentation: 奈良市(ポスター発表)
  • Year and Date: 20100913-20100916
• [Presentation] ATP-dependent transition of Hsp60 from single to double rings in the presence of cochaperonin Hsp10. (2010)
  • Author(s): Ryuichi Ishida, Toshihiko Oka, Hiroki Takahashi, Hiroshi Kubota, Hideaki Itoh
  • Organizer: The 3rd International Symposium on Protein Community
  • Place of Presentation: 奈良市(ポスター発表)
  • Year and Date: 20100913-20100916
• [Presentation] Co-chaperone HOP (HSP70/HSP90 organizing protein) has ATPase activity and changes its conformation in the presence of ATP. (2010)
  • Author(s): Soh Yamamoto, Ganesh Prasad Subedi, Shinya Hanashima, Tadashi Satoh, Yoshiki Yamaguchi, Hiroshi Kubota, Hideaki Itoh
  • Organizer: The 3rd International Symposium on Protein Community
  • Place of Presentation: 奈良市(ポスター発表)
  • Year and Date: 20100913-20100916
• [Presentation] 分子シャペロンPrefoldinはポリグルタミンの凝集と細胞毒性を減少させる (2010)
  • Author(s): 田代絵梨佳,武藤秀樹,座古保,宮澤誠,北浦廣剛,北村朗,久保田広志,前田瑞夫,金城政孝,有賀寛芳
  • Organizer: 第33回日本神経科学大会-第53回日本神経化学会大会-第20回日本神経回路学会大会-合同大会
  • Place of Presentation: 神戸市(ポスター発表)
  • Year and Date: 20100902-20100904
• [Presentation] 蛍光で見る蛋白質品質管理のダイナミクス (2010)
  • Author(s): 久保田広志
  • Organizer: 平成22年度日本分析化学会東北支部若手交流会
  • Place of Presentation: 秋田市(口頭発表)
  • Year and Date: 20100702-20100703
• [Presentation] ポリグルタミンタンパク質凝集における分子シャペロンプレフォルディンの役割 (2010)
  • Author(s): 伊藤禎宣、座古保、ソルヤード・カリン、寺田尚史、田代絵梨佳、北村朗、久保田広志、武藤秀樹、金城政孝、有賀寛芳、前田瑞夫
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 札幌市(ポスター発表)
  • Year and Date: 20100616-20100618
• [Presentation] SOD1脱凝集過程におけるタンパク質分解経路と細胞毒性の関係について (2009)
  • Author(s): 北村朗,稲田のりこ,久保田広志,松本弦,Richard I.Morimoto,金城政孝,永田和宏
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜市(口頭発表およびポスター発表)
  • Year and Date: 20091209-20091212
• [Presentation] MM-1およびPrefoldinがポリグルタミン凝集に与える影響 (2009)
  • Author(s): 田代絵梨佳,武藤秀樹,座古誠,宮澤誠,北浦廣剛,北村朗,久保田広志,前田瑞夫,金城正孝,有賀芳寛
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜市(ポスター発表)
  • Year and Date: 20091209-20091212
• [Presentation] Increased expression of HOP and chaperone complex formation in human colonic carcinoma. (2009)
  • Author(s): Hiroshi Kubota, Soh Yamamoto, Yuzo Yamamoto, Hideaki Itoh
  • Organizer: The 4th International Congress on Stress Responses in Biology and Medicine. Workshop 'Stress Responses in Disease'(Abstracts, p169)
  • Place of Presentation: 札幌市(口頭発表およびポスター発表)
  • Year and Date: 20091006-20091009
• [Presentation] The antibiotic gentamicin (GM) was specifically bound to HSP70 C-domain and inhibited HSP40/HSC70 chaperone activity. (2009)
  • Author(s): Soh Yamamoto, Shunsuke Nakano, Hiroshi Kubota, Hideaki Itoh
  • Organizer: The 4th International Congress on Stress Responses in Biology and Medicine.
  • Place of Presentation: 札幌市(ポスター発表)
  • Year and Date: 20091006-20091009
• [Presentation] Cisplatin differently affects amino terminal amd carboxyl terminal domains of HSP90. (2009)
  • Author(s): Ryuichi Ishida, Yuka Takaoka, Soh Yamamoto, Toshio Miyazaki, Michiro Otaka, Sumio Watanabe, Atushi Komatsuda, Hideki Wakui, Ken-ichi Sawada, Hiroshi Kubota, Hideaki Itoh
  • Organizer: The 4th International Congress on Stress Responses in Biology and Medicine.
  • Place of Presentation: 札幌市(ポスター発表)
  • Year and Date: 20091006-20091009
• [Presentation] Autophagy eliminates misfolded procollagen aggregates in the endoplasmic reticulum for cell survival. (2009)
  • Author(s): Yoshihito Ishida, Akitsugu Yamamoto, Akira Kitamura, Shireen R.Lamande, Tamotsu Yoshimori, John F.Bateman, Hiroshi Kubota, Kazuhiro Nagata
  • Organizer: Joint meeting of 8th Pan-Pacific Connective Tissue Societies Symposium (PPCTSS), 41st Annual Meeting of the Japanese Society of Connective Tissue Research (JSCTR), and 56th Annual Meeting of the Japan Matrix Club.
  • Place of Presentation: 横須賀市(ポスター発表)
  • Year and Date: 20090704-20090707
• [Presentation] ミトコンドリア移行シグナル付加Hsp60の生理機能解析 (2009)
  • Author(s): 石田竜一、伊豆原史子、関根和宏、大高慶、久保田広志、伊藤英晃
  • Organizer: 第9回日本蛋白質科学会年会
  • Place of Presentation: 熊本市(ポスター発表)
  • Year and Date: 20090520-20090522
• [Presentation] Fluorescently labeled proteins as a tool for analyzing the dynamics of protein quality control in living cells. (2009)
  • Author(s): Hiroshi Kubota, Akira Kitamura, Hideaki Itoh, Masataka Kinjo, Kazuhiro Nagata
  • Organizer: The Sixth Conference on Materials Engineering for Resources. Keynote session 'New Materials for Life Science-I'(Abstracts, p12)
  • Place of Presentation: 秋田市(口頭発表)
  • Year and Date: 2009-10-22
• [Presentation] Gravity-responsive transcriptional regulation of collagen-specific molecular chaperone HSP47. (2009)
  • Author(s): Asami Oguro-Ando, Hiroshi Kubota, Yoriko Atomi, Shoichi Ishiura, Kazuhiro Nagata
  • Organizer: 日本宇宙生物科学会第23回大会
  • Place of Presentation: つくば市(口頭発表)
  • Year and Date: 2009-10-02
• [Presentation] Aggregation and disaggregation of ALS-linked mutant SOD1 via soluble oligomers in living cells. (2009)
  • Author(s): Hiroshi Kubota, Akira Kitamura, Noriko Inada, Gen Matsumoto, Richard Morimoto, Masataka Kinjo, Kazuhiro Nagata
  • Organizer: 第61回日本細胞生物学会大会シンポジウム「タンパク質社会と品質管理」
  • Place of Presentation: 名古屋市(口頭発表)
  • Year and Date: 2009-06-03
• [Presentation] Dynamics of Aggregation and disaggregation of neurodegenerative disease-causative proteins (2009)
  • Author(s): Akira Kitamura, Masataka Kinjo, Hiroshi Kubota, Kazuhiro Nagata
  • Organizer: International Conference 'Protein folding and neurodegenerative disease'
  • Place of Presentation: 京都市(口頭発表)
  • Year and Date: 2009-04-06
• [Presentation] タンパク質品質管理機構の研究 (2009)
  • Author(s): 久保田広志
  • Organizer: 平成20年度京都大学再生医科学研究所共同研究会
  • Place of Presentation: 京都市(口頭発表)
  • Year and Date: 2009-03-27
• [Presentation] 分光イメージング法を用いた変異型SOD1の凝集体形成と凝集体中間構造の解析-凝集中間体と細胞毒性の関係について- (2008)
  • Author(s): 北村朗、久保田広志、稲田のりこ、松本弦、Richard I.Morimoto、金城政孝、永田和宏
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会-合同大会
  • Place of Presentation: 神戸市(一般口頭発表およびポスター発表)
  • Year and Date: 20081209-20081212
• [Presentation] 小胞体に蓄積した変異コラーゲンの分解:オートファジーとERADの使い分け (2008)
  • Author(s): 石田義人、久保田広志、北村朗、山本章嗣、John Bateman、吉森保、永田和宏
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸市(一般口頭発表およびポスター発表)
  • Year and Date: 20081209-20081212
• [Presentation] 小胞体に蓄積するミスフォールドコラーゲンの構造依存的な分解経路の仕分け (2008)
  • Author(s): 石田義人、山本章嗣、北村朗、Shireen R Lamande、吉森保、John F Bateman、久保田広志、永田和宏
  • Organizer: 第3回臨床ストレス応答学会大会
  • Place of Presentation: 秋田市
  • Year and Date: 20081114-20081115
• [Presentation] 大腸菌多機能プロテアーゼ/シャペロンHtrA/DegPのヒトホモローグの一つHteA1の発現はケロイドの病期と予後に相関する (2008)
  • Author(s): 内藤素子、久保田広志、石河利弘、吉川勝宇、山脇聖子、鈴木茂彦、永田和宏
  • Organizer: 第3回臨床ストレス応答学会大会
  • Place of Presentation: 秋田市
  • Year and Date: 20081114-20081115
• [Presentation] GGAはヘリコバクターピロリ菌dnaK(HSP70)のシャペロン活性を選択的に抑制する (2008)
  • Author(s): 大瀧貴子,山本聡,藤原一彦,小熊恵二,佐藤昇志,大高道郎,久保田広志,伊藤英晃
  • Organizer: 第3回臨床ストレス応答学会大会
  • Place of Presentation: 秋田市
  • Year and Date: 20081114-20081115
• [Presentation] Gentamicin誘導腎毒性に対するHSP70の抵抗性 (2008)
  • Author(s): 伊藤江里、山本聡、宮崎敏夫、小松敦、涌井秀樹、澤田賢一、大高道郎、久保田広志、伊藤英晃
  • Organizer: 第3回臨床ストレス応答学会大会
  • Place of Presentation: 秋田市
  • Year and Date: 20081114-20081115
• [Presentation] 哺乳類Hsp60の生化学的性質の解析 (2008)
  • Author(s): 関根和宏,石田竜一,野口晋,山本聡,大高慶,伊豆原史子,涌井秀樹,澤田賢一,大高道郎,久保田広志,伊藤英晃
  • Organizer: 第3回臨床ストレス応答学会大会
  • Place of Presentation: 秋田市
  • Year and Date: 20081114-20081115
• [Presentation] HOP ATPase活性の分子シャペロン複合体に及ぼす影響 (2008)
  • Author(s): 山本聡、藤原一彦、涌井秀樹、山本雄造、大高道郎、久保田広志、伊藤英晃
  • Organizer: 第3回臨床ストレス応答学会大会
  • Place of Presentation: 秋田市
  • Year and Date: 20081114-20081115
• [Presentation] コシャペロンHOPとAnnexinA2の相互作用解析 (2008)
  • Author(s): 尾張健介、山本聡、湧井秀樹、澤田賢一、小林良二、久保田広志、伊藤英晃
  • Organizer: 第3回臨床ストレス応答学会大会
  • Place of Presentation: 秋田市
  • Year and Date: 20081114-20081115
• [Presentation] Misfolded collagens acumulated in the ER by the absence of HSP47 are disposed via autophagy-lysosome. (2008)
  • Author(s): Yoshihito Ishida, Akitsugu Yamamoto, Akira Kitamura, Tamotsu Yoshimori, John F.Bateman, Hiroshi Kubota, Kazuhiro Nagata
  • Organizer: Cold Spring Harbor Meeting "Molecular Chaperones and stress Response"
  • Place of Presentation: Cold Spring Harbor (USA)
  • Year and Date: 20080430-20080504
• [Book] 蛋白質核酸酵素53(遠藤斗志也,小椋光,永田和宏,森和俊,田口英樹,吉田賢右編)(蛋白質品質管理,CCT キーワード:蛋白質の一生) (2008)
  • Author(s): 久保田広志
  • Total Pages: 942,967,1031
  • Publisher: シャペロニン