Production of Wnt4b by floor plate cells is essential for the segmental patterning of the vertebral column in medaka

2010 Fiscal Year Final Research Report

• Project/Area Number: 20570199
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: INOHAYA Keiji Tokyo Institute of Technology, 大学院・生命理工学研究科, 助教 (70302958)
• Project Period (FY): 2008 – 2010
• Keywords: 器官形成 / メダカ / 骨形成 / 脊椎発生 / Wnt

Research Abstract

Analysis of a spontaneous medaka mutant, fused centrum ( fsc), which exhibits fused centra and the absence of the intervertebral ligaments, revealed that fsc encodes wnt4b, which was expressed exclusively in the floor plate. In fsc mutants, wnt4b expression was completely lost in the floor plate and that abnormal conversion of the intervertebral ligament cells into osteoblasts appeared to cause a defect of the intervertebral ligaments. These findings provide a novel perspective on the mechanism of vertebrate development.

Research Products

• [Journal Article] Production of Wnt4b by floor plate cells is essential for the segmental patterning of the vertebral column in medaka. (2010)
  • Author(s): Inohaya K, Takano Y, Kudo A.
  • Journal Title: Development. 137 Pages: 1807-1813
  • Peer Reviewed
• [Journal Article] Bef medaka mutant reveals the essential role of c-myb in both primitive anc definitive hematopoiesis. (2010)
  • Author(s): Moriyama A, Inohaya K, Maruyama K, Kudc A.
  • Journal Title: Developmental Biology. 345 Pages: 133-143
  • Peer Reviewed
• [Journal Article] sec24d encoding a component of COPII is essential for vertebra formation, revealed by the analysis of the medaka mutant, vbi. (2010)
  • Author(s): Ohisa S, Inohaya K, Takano Y, Kudo A.
  • Journal Title: Developmental Biology. 342 Pages: 85-95
  • Peer Reviewed
• [Journal Article] Purification and characterization of zebrafish hatching enzyme : An evolutionary aspect of the mechanism of egg envelope digestion. (2008)
  • Author(s): Sano K, Inohaya K, Kawaguchi M, Yoshizaki N, Iuchi I, Yasumasu S.
  • Journal Title: FEBS J. 275 Pages: 5934-5946
  • Peer Reviewed
• [Journal Article] Phenotypic analyses of a medaka mutant reveal the importance of bilaterally synchronized expression of isthmic fgf8 for bilaterally symmetric formation of the optic tectum. (2008)
  • Author(s): Takashima S, Kage T, Yasuda T, Inohaya K, Maruyama K, Araki K, Takeda H, Ishikawa Y.
  • Journal Title: Genesis. 46 Pages: 537-545
  • Peer Reviewed
• [Journal Article] Function of Pax1 and Pax9 in the sclerotome of medaka fish. (2008)
  • Author(s): Mise T, Iijima M, Inohaya K, Kudo A, Wada H.
  • Journal Title: Genesis. 46 Pages: 185-192
  • Peer Reviewed
• [Journal Article] The medaka FoxP2, a homologue of human language gene FOXP2, has a diverged structure and function. (2008)
  • Author(s): Itakura T, Chandra A, Yang Z, Xue X, Wang B, Kimura W, Hikosaka K, Inohaya K, Kudo A, Uezato T, Miura N.
  • Journal Title: J.Biochem. 143 Pages: 407-416
  • Peer Reviewed
• [Presentation] フロアープレート細胞におけるwnt4bの発現は脊椎の分節機構に必須である (2009)
  • Author(s): 猪早敬二、高野吉郎、工藤明
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: 静岡
  • Year and Date: 2009-09-17
• [Presentation] Production of wnt4b by floor plate cells is essential for the segmental pattern of vertebral column (2009)
  • Author(s): 猪早敬二、高野吉郎、工藤明
  • Organizer: 日本発生生物学会第42回大会
  • Place of Presentation: 新潟
  • Year and Date: 2009-05-28
• [Presentation] 脊椎骨融合を示すダルマメダカ変異体の原因遺伝子の解明 (2008)
  • Author(s): 猪早敬二、高野吉郎、工藤明
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡
  • Year and Date: 2008-09-05
• [Presentation] Analysis of the Dharma-medaka mutant, which shows a defect in formation and/or maintenance of intervertebral region (2008)
  • Author(s): 猪早敬二、高野吉郎、工藤明
  • Organizer: 日本発生生物学会第41回大会
  • Place of Presentation: 徳島
  • Year and Date: 2008-05-28