Developmental mechanism of Drosophila male accessory gland analogous to mammalian prostate

2013 Fiscal Year Final Research Report

• Project/Area Number: 20570202
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Gakushuin University (2009-2013); Kobe University (2008)
• Principal Investigator: ADACHI-YAMADA Takashi 学習院大学, 理学部, 教授 (20221723)
• Project Period (FY): 2008-04-08 – 2013-03-31
• Keywords: ショウジョウバエ / 附属腺/前立腺 / 二核細胞 / 繁殖戦略 / 立方/扁平上皮細胞 / 細胞周期制御 / 微小管結合因子 / Mad/NuMA

Research Abstract

The accessory gland of adult male Drosophila is analogous to the mammalian prostate which produces various components in the seminal fluid. More than 80 kinds of peptidergic components are known to be important for male reproductive strategy in stimulation of various female reproductive behaviors. The accessory gland cells show a unique cellular feature in which all of the epithelial cells are binucleate cells. We demonstrated that this morphology brings a cell shape flexibility to cells to directly affect the volume of the accessory gland cavity and the storage amount of seminal fluid (Taniguchi et al. 2012). We also demonstrated that the cell cycle in the developing accessory gland was unusually synchronized around cytokinesis skipping in the pupal stage, a process of binucleation. Furthermore, we sought causal factor(s) to create binucleate cells. As a result, a previously unknown splicing variant of a microtubule–binding factor Mud/NuMA was responsible for this binucleation.

Research Products

• [Journal Article] The homeodomain protein defective proventriculus is essential for male accessory gland development to enhance fecundity in Drosophila (2012)
  • Author(s): Minami, R., Wakabayashi, M., Sugimori, S., Taniguchi, K., Kokuryo, A., Imano T., Adachi-Yamada T., Watanabe, N. and Nakagoshi, H.
  • Journal Title: PLoS One Volume: 7 Pages: e32302
• [Journal Article] Binucleation of Drosophila Adult Male Accessory Gland Cells Increases Plasticity of Organ Size for Effective Reproduction (2011)
  • Author(s): Taniguchi, K., Kokuryo, A., Imano, T., Minami, R., Nakagoshi, H. and Adachi-Yamada, T.
  • Journal Title: Biol. Syst. Volume: 1 Pages: e101
• [Presentation] Isoform-specific functions of Mud/NuMA mediate binucleation of Drosophila male accessory gland cells (2014)
  • Author(s): 谷口, 國領, 今野, 南, 中越, 安達
  • Organizer: 55rd Annual Drosophila Research Conference
  • Place of Presentation: アメリカ, サンディエゴ
  • Year and Date: 20140326-30
• [Presentation] Acquisition of apoptosis-resistances in Drosophila accessory gland exocrine cells (2013)
  • Author(s): 谷口, 安達
  • Organizer: 新学術領域研究「上皮管腔組織形成」第1回国際シンポジウム
  • Place of Presentation: 札幌
  • Year and Date: 20130622-23
• [Presentation] Mud/NuMA はショウジョウバエ雄生殖器附属腺において細胞二核化を制御する (2013)
  • Author(s): 谷口, 國領, 今野, 南, 中越, 安達
  • Organizer: 第65回日本細胞生物学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 20130619-21
• [Presentation] Elimination of malfunctioning cells in Drosophila male accessory gland, a non-regenerative tissue (2012)
  • Author(s): 谷口, 安倍, 南, 今野, 國領, 中越, 安達
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 20121211-14
• [Presentation] Cytokinesis-deficient binucleation in Drosophila accessory gland for providing plasticity of organ size (2012)
  • Author(s): 谷口, 國領, 今野, 坂田, 南, 中越, 安達
  • Organizer: BSCB/BSDB/JSDB Joint Spring Meeting 2012
  • Place of Presentation: イギリス, コヴェントリ
  • Year and Date: 20120415-18
• [Presentation] Cytokinesis-deficient binucleation in Drosophila accessory gland for providing plasticity of organ size (2012)
  • Author(s): 谷口, 國領, 今野, 坂田, 南, 中越, 安達
  • Organizer: 53rd Annual Drosophila Research Conference
  • Place of Presentation: アメリカ, シカゴ
  • Year and Date: 20120307-11
• [Presentation] Cytokinesis-deficient binucleation in Drosophila accessory gland for providing a higher level of plasticity of organ size (2011)
  • Author(s): 谷口,國領,今野,坂田,南,中越,安達
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20111213-16
• [Presentation] ショウジョウバエ Dveは附属線の細胞死阻害によってオスの繁殖力を高める (2011)
  • Author(s): 南,若林,杉森,谷口,國領,今野,安達,渡辺,中越
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20111213-16
• [Presentation] Cytokinesis-deficient binucleation in Drosophila accessory gland for providing plasticity of organ size (2011)
  • Author(s): 谷口,國領,今野,坂田,南,中越,安達
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 札幌
  • Year and Date: 20110627-29
• [Presentation] Cytokinesis-deficient binucleation in Drosophila accessory gland for providing plasticity of organ size (2011)
  • Author(s): 谷口,國領,今野,坂田,南,中越,安達
  • Organizer: 1st Asia-Pacific Drosophila Research Conference
  • Place of Presentation: 台湾,台北
  • Year and Date: 20110522-25
• [Presentation] Cytokinesis-deficient binucleation as a strategy for tissue enlargement in Drosophila (2010)
  • Author(s): 谷口,國領,今野,南,中越,安達
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 20101207-10
• [Presentation] Cytokinesis-deficient binucleation of Drosophila accessory gland cells as a strategy for better reproductive success (2010)
  • Author(s): 谷口,國領,今野,南,中越,安達
  • Organizer: 2nd Joint Meeting of the SFBD and JSDB 2010
  • Place of Presentation: フランス,パリ
  • Year and Date: 20100526-28
• [Presentation] Male fertility depends on proper differentiation of accessory gland in Drosophila (2010)
  • Author(s): 南,谷口,國領,今野,安達,中越
  • Organizer: 51st Annual Drosophila Research Conference
  • Place of Presentation: アメリカ,ワシントン
  • Year and Date: 20100407-11
• [Presentation] Analysis of binucleation in Drosophila male accessory gland cell (2009)
  • Author(s): 谷口,國領,今野,南,中越,安達
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20091209-12
• [Presentation] Centrosome dynamics and binucleation in Drosophila adult male accessory gland (2009)
  • Author(s): 今野,國領,谷口,南,中越,安達
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20091209-12
• [Presentation] Normal development of accessory gland is crucial for male fertility in Drosophila (2009)
  • Author(s): 南,谷口,國領,今野,安達,中越
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20091209-12
• [Presentation] Polo is required for proper cell differentiation of main cells in adult male accessory gland (2009)
  • Author(s): 國領,南,今野,谷口,中越,安達
  • Organizer: 日本ショウジョウバエ研究会第9回研究集会
  • Place of Presentation: 掛川
  • Year and Date: 20090706-08
• [Presentation] Male fertility depends on normal differentiation of accessory gland (2009)
  • Author(s): 南,國領,安達,中越
  • Organizer: 日本ショウジョウバエ研究会第9回研究集会
  • Place of Presentation: 掛川
  • Year and Date: 20090706-08