Integrated analysis of the foregut formation and regionalization of the endoderm

2010 Fiscal Year Final Research Report

• Project/Area Number: 20570207
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Tokyo Metropolitan University
• Principal Investigator: FUKUDA Kimiko Tokyo Metropolitan University, 大学院・理工学研究科, 准教授 (40285094)
• Project Period (FY): 2008 – 2010
• Keywords: 器官形成 / 消化管 / 内胚葉 / シグナル分子

Research Abstract

Much about molecular mechanisms involved in regionalization of the gut endoderm remain unknown. To clarify this we isolated genes expressed in the early endoderm locally, and analyzed their function. We found that sizzled modified gradient of BMP ligand and regulated liver and stomach differentiation. Also, APOA1 induced liver endoderm at the pancreas region. In addition, the mechanism of boundary formation between the stomach and the intestine were controlled by two transcriptional factors, which are expressed in each region.

Research Products

• [Journal Article] Identification of region specific genes in early chicken endoderm. (2011)
  • Author(s): Kimura, W., Cantas, A. Sheng, G. Jakt, M., Yasugi, S., Fukuda, K.
  • Journal Title: Gene Exp.Patterns in press
• [Journal Article] Conserved origin of the ventral pancreas in chicken. (2009)
  • Author(s): Matsuura, K., Katsumoto, K. Fukuda, K., Kume, K., Kume, S.
  • Journal Title: Mech.Dev. 126 Pages: 817-827
• [Journal Article] Origin of pancreatic precursors in the chick embryo and the mechanism of endoderm regionalization. (2009)
  • Author(s): Katsumoto, K., Fukuda, K., Kimura, W., Shimamura, K., Yasugi, S., Kume, S.
  • Journal Title: Mech.Dev. 126 Pages: 539-551
• [Presentation] The mechanisms of the boundary formation between the stomach and intestine endoderm in chicken embryo. (2010)
  • Author(s): 渡辺健太, 福田公子
  • Organizer: Society for Developmental Biology 69th Annual Meeting jointly with the Japanese Society of Developmental Biologists
  • Place of Presentation: Albuquerque
  • Year and Date: 20100000
• [Presentation] Sizzled in the foregut restricts liver region into anterior intestinal portal in the chicken embryo. (2010)
  • Author(s): Okayama, Y, Kimura W., Yasugi S., Fukuda K.
  • Organizer: 43^<rd> Annual Meeting for the Japanese Society for Developmental Biologists jointly sponsored by the Asia-Pacific Developmental Biology Network
  • Place of Presentation: Kyoto
  • Year and Date: 20100000
• [Presentation] ニワトリ胚内胚葉において胃/腸の境界形成に関わるSox2とCdxAの相互抑制作用 Repressive interaction between Sox2 and CdxA is involved in the establishment of stomach/intestine boundary in chicken embryonic endoderm. (2009)
  • Author(s): 渡辺健太, 福田公子
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20090000
• [Presentation] ニワトリ胚における消化管上皮分化に関わる遺伝子の探索 (2008)
  • Author(s): 武政智恵, 木村航, 八杉貞雄, 福田公子
  • Organizer: 第31回日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 20080000
• [Book] Electroporation of nucleic Acids into chick endoderm both in vitro and in ovo.(Electroporation and sonoporation in developmental biology)(H. Nakamura ed.) (2009)
  • Author(s): Kimiko Fukuda
  • Publisher: Springer