2010 Fiscal Year Final Research Report
Functional analysis of microRNAs on sex determination and differentiation
| Project/Area Number |
20570210
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Developmental biology
|
|---|
| Research Institution | Jichi Medical University |
|---|
Principal Investigator |
TAKADA Shuji Jichi Medical University, ゲノム機能研究室, 室長 (20382856)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Keywords | マウス / microRNA / 生殖腺 / 性決定 / 性分化 |
|---|
| Research Abstract |
To examine if microRNA plays a role in gonad formation and sexual differentiation, microRNA profiles were determined by high-sensitive microRNA profiling method, mRAP, using mouse embryonic supporting cells purified from male and female gonads. The profiles obtained were further verified by TaqMan method. The results shows that mir-199a-5p is preferentially expressed in female supporting cells, suggesting that microRNA is associated with gonad formation and sexual differentiation in supporting cells in mouse embryonic gonad.
|
