2010 Fiscal Year Final Research Report
Analysis of cell wall degrading enzyme of a methanogenic archaeon, Methanosarcina mazei, and utilization of its functions
| Project/Area Number |
20580076
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Nagoya University |
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Principal Investigator |
ASAKAWA Susumu Nagoya University, 生命農学研究科, 准教授 (50335014)
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| Project Period (FY) |
2008 – 2010
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| Keywords | メタン生成古細菌 / 細胞壁分解酵素 / 形態変化 / 絶対嫌気性 / Methanosarcina mazei / disaggregatase / aggregate / single cell |
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| Research Abstract |
The objective of this study was to elucidate conditions for expression of cell wall degrading enzyme, disaggregatase, involved in morphological changes from aggregates to single cells of a methanogenic archaeon, Methanosarcina mazei, and to obtain fundamental information to utilize functions of the enzyme for mitigation of methane emission and improvement of methane fermenting efficiency. Activity of disaggregatase in Methanosarcina mazei was expressed by reducing the proteins in solutions with appropriate concentrations of cations. Rice plants introduced with the disaggregatase gene successfully transcribed the gene. Relationships between expression of disaggregatase and morphological change from aggregates to single cells varied with two strains of Methanosarcina mazei, indicating a clue to reveal mechanism of the morphological changes in Methanosarcina mazei.
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