2010 Fiscal Year Final Research Report
Development of discrimination method between Dojou and Kara-dojou loaches using DNA markers for conservation of rice paddy ecosystem
| Project/Area Number |
20580270
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Irrigation, drainage and rural engineering/Rural planning
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| Research Institution | National Agricultural Research Organization |
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Principal Investigator |
KOIZUMI Noriyuki National Agricultural Research Organization, 農村環境部・生態工学研究室, 主任研究員 (60301222)
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| Project Period (FY) |
2008 – 2010
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| Keywords | ドジョウ / カラドジョウ / ミトコンドリアDNA / 核DNA / 判別式 / 生きもの調査 |
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| Research Abstract |
Two loaches, the Dojou Misgurnus anguillicaudatus and Kara-dojou Paramisgurnus dabryanus inhabiting in rice paddies may be discriminated each other using mitochondrial and nuclear DNA markers in this study. Applying mitochondrial DNA analysis, the Dojou was separated into four genetic clades, Europe, China, Korea and Japan types. The Kara-dojou formed a single clade, Kara-dojou type. Results of nuclear DNA analysis showed the possibility of no hybridizing individual of them. To use a species discrimination equation with valuables of barbell length and body height could be available to decrease in a number of specimens used in the DNA analysis.
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