2010 Fiscal Year Final Research Report
Improvement of the developmental capacity of nuclear transfer embryos derived from gene-modified clawn miniature pig cells using a histone deacetylase inhibitor and latrunculin A.
| Project/Area Number |
20580311
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Kagoshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MIYOSHI Kazuchika 鹿児島大学, 農学部, 准教授 (70363611)
SATO Masahiro 鹿児島大学, フロンティアサイエンス研究推進センター, 教授 (30287099)
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| Research Collaborator |
HIMAKI Takehiro 鹿児島大学, 大学院・連合農学研究科, 学生
MORI Hironori 鹿児島大学, 大学院・農学研究科, 学生
TSUDA Kennichirou 鹿児島大学, 大学院・農学研究科, 学生
MIZOBE Yamato 鹿児島大学, 大学院・農学研究科, 学生
SUETOMO Masashi 鹿児島大学, 大学院・農学研究科, 学生
OGAWA Jyunki 鹿児島大学, 大学院・農学研究科, 学生
AKAHOSHI Kaoru 鹿児島大学, 農学部, 学生
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| Project Period (FY) |
2008 – 2010
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| Keywords | 体細胞クローン / 遺伝子改変 / 脱アセチル化 / トリコスタチンA / スベロイルビスヒドロキサム酸 / スクリパタイド / ラトランキュリンA / クラウン系ミニブタ |
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| Research Abstract |
The objective of this study was to examine the effects of postactivation treatment of histone deacetylase inhibitors(HADCi) and an actin polymerisation inhibitor, latrunculin A(LatA) on in vitro and in vivo development of somatic cell nuclear transfer(SCNT) embryos derived from gene^modified Clawn miniature pig cells. After electric activation, SCNT embryos were treated with or without LatA, and cultured in vitro. The rate of blastocyst formation was significantly higher(p<0. 05) in SCNT embryos treated with HADCi and 0. 5μM LatA than that in control. In addition, fetuses and piglets were obtained from recipient uteri after transfer of embryos treated with HDACi and LatA. The results of this study showed for the first time that postactivation treatment with HDACi and LatA was effective to improve in vitro and in vivo developmental capacity of gene-modified cloned miniature pig embryos.
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