Molecular analysis of Rcd1 protein as transcriptional cofactor, which may play a important role in the transformation of muscle cells

2010 Fiscal Year Final Research Report

• Project/Area Number: 20580320
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Gifu University
• Principal Investigator: NAGANO Isao 岐阜大学, 医学系研究科, 准教授 (40283296)
• Co-Investigator(Kenkyū-buntansha): WU Zhiliang 岐阜大学, 医学系研究科, 助教 (90313874)
• Project Period (FY): 2008 – 2010
• Keywords: 旋毛虫 / 筋肉細胞変異 / 筋肉細胞分化 / Rcd1 / AP-1 / NF-κB / myogenin / MyoD

Research Abstract

Trichinella spp. infection causes satellite cell proliferation and transformation of muscle cell to the nurse cell. Rcd1 is shown to be a transcriptional cofactor of the c-myb proto-oncogene product. Previously, we cloned Rcd1 gene from the cDNA of T. spiralis muscle larvae, and confirmed that the Rcd1 protein is part of the E-S products. Therefore, the Rcd1 likely affects host cells and tissues for capsule formation. In the present study we investigated the effect by which the Rcd1protein secreted from T. spiralis modulates myogenic differentiation in C2C12 myoblasts, and the role of the Rcd1 protein regulated NF-κB and AP-1 transcriptional pathway. The expression of myogenin and MyoD genes in C2C12 myoblasts transfected with Rcd1 gene was significantly lower than those in non-transfected C2C12 myoblasts. And we demonstrate that Rcd1 is a potent inhibitor of muscle differentiation through activation(C2C12 cells) of NF-κB and AP-1 signaling pathway. It may be inferred from these results that the Rcd1 protein functions as a transcriptional factor or cofactor of satellite cell proliferation and muscle cell transformation.

Research Products

• [Journal Article] Molecular cloning and characterization of transgelin-like proteins mainly transcribed in newborn larvae of Trichinella spp (2011)
  • Author(s): Nagano, I., Wu, Z., Asano, K. & Takahashi, Y.
  • Journal Title: Veterinary Parasitology Volume: 178(1-2) Pages: 134-142
  • Peer Reviewed
• [Journal Article] Infection of non-encapsulated species of Trichinella ameliorates experimental autoimmune encephalomyelitis involving suppression of Th17 and Th1 response (2010)
  • Author(s): Wu, Z., Nagano, I., Asano, K., & Takahashi, Y.
  • Journal Title: Parasitology Research Volume: 107(5) Pages: 1173-1188
  • Peer Reviewed
• [Journal Article] Human trichinosis after consumption of soft-shelled turtles, Taiwan (2009)
  • Author(s): Lo, Y. C., Hung, C. C., Lai, C. S., Wu, Z., Nagano, I., Maeda, T., Takahashi, Y., Chiu, C. H., & Shyong Jiang, D. D.
  • Journal Title: Emerging Infectious Diseases Volume: 15(12) Pages: 2056-2058
  • Peer Reviewed
• [Journal Article] Functional genes and proteins of Trichinella spp (2009)
  • Author(s): Nagano, I., Wu, Z. & Takahashi, Y.
  • Journal Title: Parasitology Research Volume: 104(2) Pages: 197-207
  • Peer Reviewed
• [Journal Article] Hypoglycaemia induced by Trichinella infection is due to the increase of glucose uptake in infected muscle cells (2008)
  • Author(s): Wu, Z., Nagano, I., Kajita, K., Nishida, M. & Takahashi, Y.
  • Journal Title: International Journal for Parasitology Volume: 39(4) Pages: 427-434
  • Peer Reviewed
• [Journal Article] Trichinella spiralis : nurse cell formation with emphasis on analogy to muscle cell repair (2008)
  • Author(s): Wu, Z., Nagano, I., & Takahashi, Y.
  • Journal Title: Parasite and vector Volume: 19(1) Pages: 27
  • Peer Reviewed
• [Presentation] Rcd1 protein secreted from Trichinella spiralis down-regulates myogenin and MyoD proteins in C2C12 myoblasts, and inhibits binding activities of transcription factors NF-κB and AP-1 (2011)
  • Author(s): Isao Nagano
  • Organizer: 13th International Conference on Trichinellosis
  • Place of Presentation: Changchun, China
  • Year and Date: 2011-08-16
• [Presentation] 種特異的組換え抗原を用いた旋毛虫症の血清学的診断法の検討 (2009)
  • Author(s): 長野功
  • Organizer: 第65回日本寄生虫学会西日本支部総会
  • Place of Presentation: 大阪市
  • Year and Date: 2009-11-07
• [Presentation] ELISAにおけるTrichinellaの53kDa組換えタンパク質と感染マウス血清との反応性の検討 (2009)
  • Author(s): 長野功
  • Organizer: 第78回日本寄生虫学会大会
  • Place of Presentation: 東京都
  • Year and Date: 2009-03-27
• [Book] Resent Advances in Medical Sciences(Parasites and their Human and Animal Hosts) (2010)
  • Author(s): 長野功
  • Total Pages: 111-114
  • Publisher: 第65回日本寄生虫学会西日本支部大会事務局
• [Patent(Industrial Property Rights)] 旋毛虫の種特異的抗原;および該抗原を利用した旋毛虫感染の検査法 (2011)
  • Inventor(s): 長野功,高橋優三
  • Industrial Property Rights Holder: 国立大学法人岐阜大学
  • Industrial Property Number: 特許権、特許第4830106号
  • Acquisition Date: 2011-09-30