Pathophysiological roles of signal transductions between cholinergic nerves, interstitial cells and smooth muscle cells in overactive bladder.

2010 Fiscal Year Final Research Report

• Project/Area Number: 20580322
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Gifu University
• Principal Investigator: UNNO Toshihiro Gifu University, 応用生物科学部, 教授 (90252121)
• Co-Investigator(Kenkyū-buntansha): KOMORI Seiichi 岐阜大学, 応用生物科学部, 教授 (70195866) ; MATSUYAMA Hayato 岐阜大学, 応用生物科学部, 助教 (80345800)
• Research Collaborator: SAKAI Hiroki 岐阜大学, 応用生物科学部, 准教授 ; SAKAMOTO Takashi 岐阜大学, 大学院・連合獣医学研究科, 博士課程 ; YAMAMOTO Masayuki 岐阜大学, 大学院・連合獣医学研究科, 博士課程 ; TANAHASHI Yasuyuki 岐阜大学, 大学院・連合獣医学研究科, 博士課程 ; KOIDE Kento 岐阜大学, 応用生物科学部, 学部学生 ; ICHIMURA Roshirou 岐阜大学, 応用生物科学部, 学部学生 ; ANDOH Kumiko 岐阜大学, 応用生物科学部, 学部学生 ; KIMURA Kaori 岐阜大学, 応用生物科学部, 学部学生 ; SUGIYAMA Kyoko 岐阜大学, 応用生物科学部, 学部学生 ; MATSUI Ryuusuke 岐阜大学, 応用生物科学部, 学部学生 ; KATSURADA Taisuke 岐阜大学, 応用生物科学部, 学部学生
• Project Period (FY): 2008 – 2010
• Keywords: 過活動膀胱 / コリン作動性神経 / 間質細胞 / 膀胱平滑筋細胞 / ムスカリン受容体

Research Abstract

In the present study, we attempted to clarify the phathogenetical mechanisms for the development of overactive bladder and investigated contractile responses of bladder smooth muscles to electrical field stimulation of intrinsic nerves in model mouse of overactive bladder. The results suggest that a component of bladder contraction responsible for unknown nerve stimulation was increased in the model mouse and that this unknown component may be a useful target for the new drugs for the treatment of overactive bladder.

Research Products

• [Journal Article] Nitrergic inhibition of tachykininergic neuro-muscular transmission via cyclic GMP in the hamster ileum. (2011)
  • Author(s): Matsuyama, H., Unno, T., Komori, S., Takewaki T.
  • Journal Title: J.Vet.Med.Sci. 73 Pages: 453-458
  • Peer Reviewed
• [Journal Article] Muscarinic receptor subtypes mediating Ca2+ sensitization of intestinal smooth muscle contraction; studies with mutant mice lacking the receptor subtypes. (2010)
  • Author(s): Suguro, M., Matsuyama, H., Unno, T., Tanahashi, Y., Kitazawa, T., Yamada, M., Komori. S.
  • Journal Title: J.Vet.Med.Sci. 72 Pages: 443-451
  • Peer Reviewed
• [Journal Article] Ouabain exacerbates botulinum neurotoxin-induced muscle paralysis via progression of muscle atrophy in mice. (2010)
  • Author(s): Fujikawa, R., Muroi, Y., Unno, T., Ishii, T.
  • Journal Title: J.Toxicol.Sci. 35 Pages: 795-805
  • Peer Reviewed
• [Journal Article] Multiple muscarinic pathways mediate the suppression of voltage-gated Ca2+ channels in mouse intestinal smooth muscle cells. (2009)
  • Author(s): Tanahashi, Y., Unno T., Matsuyama, H., Ishii, T., Yamada, M., Wess, J., Komori, S.
  • Journal Title: Br.J.Pharmacol. 158 Pages: 1874-1883
  • Peer Reviewed
• [Journal Article] M3 muscarinic receptors mediate positive inotropic responses in mouse atria : a study with muscarinic receptor knockout mice. (2009)
  • Author(s): Kitazawa, T., Asakawa, K., Nakamura, T., Teraoka, H., Unno, T., Komori, S., Yamada, M., Wess, J.
  • Journal Title: J.Pharmacol.Exp.Ther. 330 Pages: 487-493
  • Peer Reviewed
• [Journal Article] Tributyltin-induced Ca2+ mobilization via L-type voltage-dependent Ca2+ channels in PC12 cells. Environ.Toxicol. (2009)
  • Author(s): Unno, T., Iida, R., Okawa, M., Matsuyama, H., Hossain, M.M., Kobayashi, H., Komori, S.
  • Journal Title: Pharmacol. 28 Pages: 70-77
  • Peer Reviewed
• [Journal Article] Two types of cation channel activated by stimulation of muscarinic receptors in guinea-pig urinary bladder smooth muscle. (2008)
  • Author(s): Yamamoto, M., Unno, T., Matsuyama, H., Kohda, M., Masuda, N., Nishimura, M., Ishii, T., Komori, S.
  • Journal Title: J.Pharmacol.Sci. 108 Pages: 248-257
  • Peer Reviewed
• [Journal Article] A non-selective cationic channel activated by diacylglycerol in mouse intestinal myocytes. (2008)
  • Author(s): Sakamoto, T., Matsuyama, H., Yamamoto, M., Tanahashi, Y., Kitazawa, T., Taneike, T., Komori, S., Unno, T.
  • Journal Title: Ear.J.Pharmacol. 599 Pages: 54-57
  • Peer Reviewed
• [Journal Article] Differential presynaptic actions of pyrethroid insecticides on glutamatergic and GABAergic neurons in the hippocampus. (2008)
  • Author(s): Hossain, M.M., Suzuki, T., Unno, T., Komori, S., Kobayashi, H.
  • Journal Title: Toxicol. 243 Pages: 155-163
  • Peer Reviewed
• [Journal Article] Muscarinic receptor subtypes involved in carbachol-induced contraction of mouse uterine smooth muscle. Naunyn Schmiedebergs Arch. (2008)
  • Author(s): Kitazawa, T., Hirama, R., Masunaga, K., Nakamura, T., Asakawa, K., Cao, J., Teraoka, H., Unno, T., Komori, S., Yamada, M., Wess, J., Taneike, T.
  • Journal Title: Pharmacol. 377 Pages: 503-513
  • Peer Reviewed
• [Presentation] ハムスター小腸のタキキニン作動性神経-平滑筋間の情報伝達に対するNO作動性神経による抑制機構 (2011)
  • Author(s): 松山勇人, 海野年弘, 坂本貴史, 棚橋靖行, 山田真久, Jurgen Wess, 小森成一
  • Organizer: 第151回日本獣医学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2011-03-30
• [Presentation] Role of M3 muscarinic receptor-coupled signalling molecule in the activation of muscarinic cation channels in mouse deal smooth musclecells. (2011)
  • Author(s): Unno,T., Inasaki,M., Matsuyama H., Tanahashi Y., Kitazawa T., Yamada M., Wess, J., Komori S.
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-23
• [Presentation] マウス回腸縦走筋標本におけるムスカリン作動性収縮に対するカハール細胞の役割 (2010)
  • Author(s): 棚橋靖行、市村嘉郎、木村佳織、海野年弘、松山勇人、飯野哲、小森成一
  • Organizer: 第150回日本獣医学会学術集会
  • Place of Presentation: 帯広
  • Year and Date: 2010-09-16
• [Presentation] マウス回腸平滑筋細胞のムスカリン作動性陽イオンチャネルの活性化におけるM3受容体サブタイプに連関した情報伝達分子の役割 (2010)
  • Author(s): 海野年弘、稲崎倫子、松山勇人、棚橋靖行、北澤多喜雄、山田真久、Jurgen Wess、小森成一
  • Organizer: 第150回日本獣医学会学術集会
  • Place of Presentation: 帯広
  • Year and Date: 2010-09-16
• [Presentation] 消化管におけるコリン作動性神経-平滑筋間の情報伝達機構-ムスカリン作動性陽イオンチャネルの活性化制御を中心として- (2010)
  • Author(s): 海野年弘、北澤多喜雄、松山勇人、棚橋靖行、小森成一
  • Organizer: 第149回日本獣医学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-26
• [Presentation] Functional roles of M2 and M3 muscarinic receptors in neurogenic contractions in mouse bladder. (2010)
  • Author(s): Unno, T., Yamamoto, M., Hayashi, T., Koide, K., Matsuyama H., Yamada M., Wess, J., Komori S.
  • Organizer: 第83回日本薬理学会年会
  • Place of Presentation: 大阪
  • Year and Date: 2010-03-17
• [Presentation] マウス結腸機能におよぼすアセチルコリンームスカリン受容体経路の重要性 (2009)
  • Author(s): 中島美和、北澤多喜雄、海野年弘、小森成一, 山田真久, Jurgen Wess
  • Organizer: 第148回日本獣医学会学術集会
  • Place of Presentation: 鳥取
  • Year and Date: 2009-09-27
• [Presentation] Functional roles of M2 and M3 muscarinic receptors in cholinergic nerve-induced contractions in mouse bladder studied with receptor knockout mice. (2009)
  • Author(s): Unno, T., Yamamoto, M., Hayashi, T., Koide, K., Tanahashi Y.,. Matsuyama H., Yamada M., Wess, J., Komori, S.
  • Organizer: 6th Int.Society of Auton. Neurosci.
  • Place of Presentation: Australia.
  • Year and Date: 2009-09-02
• [Presentation] Role of M2 and M3 muscarinic receptor subtypes in mediating the cholinergic excitatory junction potentials in mouse ileum. (2009)
  • Author(s): Matsuyama H., Unno, T., Sakamoto, T., Tanahashi Y., Yamada M., Wess, J., Komori S.
  • Organizer: 6th Int.Society of Auton. Neurosci.
  • Place of Presentation: Australia.
  • Year and Date: 2009-09-02
• [Presentation] Myenteric interstitial cells of Cajal and M3 muscarinic receptors regulate the periodicity of peristaltic movements in mouse small intestine. (2009)
  • Author(s): Tanahashi Y., Waki, N.,Unno, T., Matsuyama H., Yamada M., Wess, J., Komori, S.
  • Organizer: 6th Int.Society of Auton. Neurosci.
  • Place of Presentation: Australia.
  • Year and Date: 2009-09-02
• [Presentation] Three distinct muscarinic pathways mediating cholinergic EJPs in mouse ileum. (2009)
  • Author(s): Matsuyama H., Unno, T., Yamada M., Wess, J., Komori, S.
  • Organizer: 36th Tat.congress of Physiol.Sci.(IUPS 2009).
  • Place of Presentation: 京都
  • Year and Date: 2009-07-27
• [Presentation] Myenteric interstitial cells of cajal and M3 muscarinic receptors regulate the periodicity of peristaltic movements in mouse small intestine. (2009)
  • Author(s): Tanahashi Y., Waki, N., Unno, T., Matsuyama H., Yamada M., Wess, J., Komori, S.
  • Organizer: 36th Int.congress of Physiol.Sci.(IUPS 2009).
  • Place of Presentation: 京都
  • Year and Date: 2009-07-27
• [Presentation] Functional muscarinic receptor subtypes involved in the regulation of colonic motor function in mice. (2009)
  • Author(s): Nakajima, M., Kitazawa T., Unno, T., Komori, S., Yamada, M., Wess, J.
  • Organizer: Post-genomic Advances in the Physiology of Smooth Muscle.
  • Place of Presentation: 名古屋
  • Year and Date: 2009-07-23
• [Presentation] Three distinct muscarinic pathways mediate the suppression of voltage-gated Ca2+ channels in mouse intestinal smooth muscle cells. (2009)
  • Author(s): Tanahashi Y., Unno, T., Matsuyama H., Yamada M., Wess, J., Komori, S.
  • Organizer: Post-genomic Advances in the Physiology of Smooth Muscle.
  • Place of Presentation: 名古屋
  • Year and Date: 2009-07-23
• [Presentation] マウス小腸のコリン作動性神経-平滑筋の情報伝達を仲介するムスカリン受容体サブタイプ. (2009)
  • Author(s): 松山勇人, 海野年弘, 坂本貴史, 棚橋靖行, 山田真久, Jurgen Wess, 小森成一
  • Organizer: 第51回日本平滑筋学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-07-22
• [Presentation] マウス膀胱平滑筋の神経刺激で誘発される収縮反応におけるM2およびM3ムスカリン受容体サブタイプの役割 (2009)
  • Author(s): 海野年弘, 林利美, 小出健人, 松山勇人, 山田真久, Jurgen Wess, 小森成一
  • Organizer: 第147回日本獣医学会学術集会
  • Place of Presentation: 栃木
  • Year and Date: 2009-04-04
• [Presentation] マウス回腸の興奮性接合部電位を仲介しているM2とM3ムスカリン受容体サブタイプの役割-受容体欠損マウスを用いた研究 (2009)
  • Author(s): 松山勇人, 海野年弘, 山田真久, Jurgen Wess, 小森成一
  • Organizer: 第82回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-03-16
• [Presentation] マウス膀胱平滑筋におけるM2またはM3ムスカリン受容体刺激による収縮の発現機序 (2009)
  • Author(s): 山本正行, 林利美, 松山勇人, 山田真久, Jurgen Wess, 海野年弘, 小森成一
  • Organizer: 第82回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-03-16
• [Presentation] マウス回腸の蠕動運動の発現におけるM2およびM3ムスカリン受容体サブタイプの役割 (2009)
  • Author(s): 棚橋靖行, 脇尚子, 海野年弘, 松山勇人, 山田真久, Jurgen Wess, 小森成一
  • Organizer: 第82回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-03-16
• [Presentation] M3ムスカリン受容体を介するマウス心房筋収縮増大作用 (2009)
  • Author(s): 北澤多喜雄, 浅川幸一, 寺岡宏樹, 海野生弘, 小森成一, 山田真久, Jurgen Wess
  • Organizer: 第82回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-03-16
• [Remarks] ホームページ等
  • URL: http://wwwl.gifu-u.ac.jp/~pharmaco/index.htm