2010 Fiscal Year Final Research Report

Development of vaccine candidate by using global regulator gene-deficient strains of Streptococcus suis.

Research Project

Project/Area Number	20580349
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Applied veterinary science
Research Institution	National Agriculture and Food Research Organization
Principal Investigator	OSAKI Makoto National Agriculture and Food Research Organization, 動物衛生研究所・細菌・寄生虫病研究チーム, 主任研究員 (80355164)
Co-Investigator(Renkei-kenkyūsha)	SEKIZAKI Tsutomu 東京大学, 農学部, 教授 (70355163) TAKAMATSU Daisuke 独立行政法人農業・食品産業技術総合研究機構, 動物衛生研究所・細菌・寄生虫病研究チーム, 主任研究員 (60414728)
Project Period (FY)	2008 – 2010
Keywords	疾病予防・制御 / 獣医細菌学 / 分子遺伝学 / ワクチン開発
Research Abstract	Streptococcus suis is an important pathogen in pig industry and also a causative agent of zoonosis. In this study, we generated deletion mutants of several global regulator genes from virulent strains of S. suis. One of those strains, a caseinolytic protease gene (clpP)-deletion mutant derived from S. suis P1/7 strain, was shown to be attenuated in pigs and possess ability for colonization at tonsil of pigs. Our results suggest that the S. suis clpP-deletion mutant strain can be a live-vaccine candidate for S. suis infection in pigs.