The regulatory mechanisms of retinal photoreceptor development

2010 Fiscal Year Final Research Report

• Project/Area Number: 20590079
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Osaka Bioscience Institute
• Principal Investigator: KOIKE Chieko Osaka Bioscience Institute, 薬学部, 准教授 (80342723)
• Project Period (FY): 2008 – 2010
• Keywords: 発生分化 / 神経科学

Research Abstract

We performed transgenic mice based promoter analysis to identify which sequence determines the restricted Crx expression. We identified 14bp sequence, named as CUE sequence, in the proximal region of Crx promoter that is required for the specific expression of Crx in photoreceptors. The lack of CUE sequence in Crx promoter resulted in disappearance of lacZ expression in the photoreceptor and pineal grand.

Research Products

• [Journal Article] TRPM1 mutations are associated with the complete form of congenital stationary night blindness. (2010)
  • Author(s): Makoto Nakamura, Rikako Sanuki, Tatsuhiro R.Yasuma, Akishi Onishi, Koji M.Nishiguchi, Chieko Koike, Mikiko Kadowaki, Mineo Kondo, Yozo Miyake, Takahisa Furukawa
  • Journal Title: Mol.Vis. 16 Pages: 425-37
  • Peer Reviewed
• [Journal Article] TRPM1 : A vertebrate TRP channel responsible for retinal ON bipolar function. (2010)
  • Author(s): Chieko Koike, Tomohiro Numata, Hiroshi Ueda, Yasuo Mori, Takahisa Furukawa
  • Journal Title: Cell Calcium(Review). 48(2-3) Pages: 95-101
  • Peer Reviewed
• [Journal Article] Panky, a novel photoreceptor-specific ankyrin repeat protein, is a transcriptional cofactor that suppresses CRX-regulated photoreceptor genes. (2010)
  • Author(s): Sanuki R, Omori Y, Koike C, Sato S, Furukawa T
  • Journal Title: FEBS Lett. 19;584(4) Pages: 753-8
  • Peer Reviewed
• [Journal Article] TRPM1 is a component of the retinal ON bipolar cell transduction channel in the mGluR6 cascade. (2010)
  • Author(s): Chieko Koike, Takehisa Obara, Yoshitsugu Uriu, Tomohiro Numata, Rikako Sanuki, Kentaro Miyata, Toshiyuki Koyasu, Shinji Ueno, Kazuo Funabiki, Akiko Tani, Hiroshi Ueda, Mineo Kondo, Yasuo Mori, Masao Tachibana, Takahisa Furukawa
  • Journal Title: Proc.Natl.Acad.Sci.USA 107(1) Pages: 333-337
  • Peer Reviewed
• [Journal Article] 網膜視細胞の細胞極性と細胞内オルガネラの制御-外節形成、繊毛内輸送、核のポジショニングから網膜変性症まで- (2008)
  • Author(s): 小池千恵子,古川貴久
  • Journal Title: 生化学 80 Pages: 224-232
• [Presentation] 網膜ON型双極細胞の視覚伝達チャネルTRPM1の発現メカニズム解析 (2011)
  • Author(s): 遠藤拓真、上田祐輔、多賀駿、古川貴久、小池千恵子
  • Organizer: 日本薬学会第131年会(地震のため中止・学会は成立)
  • Place of Presentation: 静岡県立大学(静岡県)
  • Year and Date: 2011-03-30
• [Presentation] マウス網膜発生過程におけるIRSp53の発現機能解析 (2011)
  • Author(s): 山岡昭士、末次志郎、小池千恵子
  • Organizer: 日本薬学会第131年会(地震のため中止・学会は成立)
  • Place of Presentation: 静岡県立大学(静岡県)
  • Year and Date: 2011-03-29
• [Presentation] 三次元マイクロプローブ電極アレイを用いたマウス網膜光応答解析 (2011)
  • Author(s): 多賀駿、殿村渉、寺前彰二、小西聡、小池千恵子
  • Organizer: 日本薬学会第131年会(地震のため中止・学会は成立)
  • Place of Presentation: 静岡県立大学(静岡県)
  • Year and Date: 2011-03-29
• [Presentation] Electroretinogram recordings inside isolated mouse retina using spatially arranged microelectrodes (2011)
  • Author(s): W.Tonomura, S.Taga, C.Koike, S.Konishi
  • Organizer: 国際ワークショップ
  • Place of Presentation: 東京大学武田ホール(東京都)
  • Year and Date: 2011-03-04
• [Presentation] TRPM1 is a component of the retinal ON bipolar cell transduction channel (2010)
  • Author(s): Chieko Koike, Tomohiro Numata, Hiroshi Ueda, Mineo Kondo, Masao Tachibana, Takahisa Furukawa
  • Organizer: The American Society of Cell Biology
  • Place of Presentation: Philadelphia(U.S.A)
  • Year and Date: 2010-12-13
• [Presentation] Rod bipolar cells in mGluR6-null mice are hyperpolarized (2010)
  • Author(s): Ying Xu, Marie Fina, 小池千恵子, Takahisa Furukawa, Noga Vardi
  • Organizer: Society for Neuroscience
  • Place of Presentation: San Diego(U.S.A)
  • Year and Date: 2010-11-17
• [Presentation] The function of mouse and human TRPM1 (2010)
  • Author(s): Chieko Koike, Tomohiro Numata, Hiroshi Ueda, Mineo Kondo, Masao Tachibana, Takahisa Furukawa
  • Organizer: FASEB meeting
  • Place of Presentation: Vermont(U.S.A)
  • Year and Date: 2010-07-21
• [Presentation] 網膜ON型双極細胞の視覚伝達チャネルTRPM1 (2010)
  • Author(s): 小池千恵子
  • Organizer: 第157回大阪大学 生物科学セミナー
  • Place of Presentation: 大阪大学(大阪府)
  • Year and Date: 2010-06-09
• [Presentation] TRPM1 is a component of the retinal ON bipolar cell transduction channel (2009)
  • Author(s): Chieko Koike, Tomohiro Numata, Hiroshi Ueda, Mineo Kondo, Masao Tachibana, Takahisa Furukawa
  • Organizer: Society for Neuroscience
  • Place of Presentation: Chicago(U.S.A)
  • Year and Date: 2009-10-21
• [Remarks] ホームページ等