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2010 Fiscal Year Final Research Report

Cytoprotection against necrotic cell death by the stabilization of the lysosome

Research Project

  • PDF
Project/Area Number 20590211
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General physiology
Research InstitutionFukushima Medical University

Principal Investigator

HAZAMA Akihiro  福島県立医科大学, 医学部, 教授 (60218394)

Co-Investigator(Kenkyū-buntansha) KATSUDA Shin-ichiro  福島県立医科大学, 医学部, 准教授 (80285022)
MIYAKE Masao  福島県立医科大学, 医学部, 助教 (00381385)
KOBAYASHI Daisuke  福島県立医科大学, 医学部, 助教 (40528080)
Project Period (FY) 2008 – 2010
Keywords細胞死 / ネクローシス / 塩素イオン / リソソーム
Research Abstract

We visualized the lysosome of HeLa cells using pepstatine or lysosensor. The lysosomal signal disappeared by the necrotic cell damage. The replacement of Cl-by other anion or the application of Cl-channel blocker protected the lysosomal signal against the necrotic cell damage. These results indicate that the inhibition of Cl-fluxes has the cytoprotective effect via the stabilization of liposome.

  • Research Products

    (8 results)

All 2012 2011 2010

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (4 results)

  • [Journal Article] Plasma membrane aquaporin AqpZ protein is essential for glucose metabolism during photomixotrophic growth of Synechocystis2011

    • Author(s)
      Akai M, Onai K, Kusano M, Sato M, Redestig H, Toyooka K, Morishita M, Miyake H, Hazama A, Checchetto V, Szabo I, Matsuoka K, Saito K, Yasui M, Ishiura M, Uozumi N.
    • Journal Title

      J Biol Chem

      Volume: 15(286) Pages: 25224-35

    • Peer Reviewed
  • [Journal Article] Improved islet yield and function by use of a chloride channel blocker during collagenase digestion2011

    • Author(s)
      Anazawa T, Sato Y, Saito T, Tsuchiya T, Kenjo A, Kimura T, Haga J, Miyake M, Waguri S, Hazama A, Gotoh M.
    • Journal Title

      Transplantation

      Volume: 27(92) Pages: 871-7

    • Peer Reviewed
  • [Journal Article] A tissue-engineered trachea derived from a framed collagen scaffold, gingival fibroblasts and adipose-derived stem cells.2010

    • Author(s)
      Kobayashi K, Suzuki T, Nomoto Y, Tada Y, Miyake M, Hazama A, Wada I, Nakamura T, Omori K.
    • Journal Title

      Biomaterials

      Volume: 31(18) Pages: 4855-63

    • Peer Reviewed
  • [Journal Article] The effect of topical amiloride eye drops on tear quantity in rabbits2010

    • Author(s)
      Hara S, Hazama A, Miyake M, Kojima T, Sasaki Y, Shimazaki J, Dogru M, Tsubota K.
    • Journal Title

      Mol Vis

      Volume: 4(16) Pages: 2279-85

    • Peer Reviewed
  • [Presentation] ウシ卵細胞における膜電位分布解析2012

    • Author(s)
      三宅将生、宮村元晴、濱野晴三、兼子智、挾間章博
    • Organizer
      日本生理学会大会
    • Year and Date
      20120000
  • [Presentation] セシウムは細胞増殖を抑制する2012

    • Author(s)
      小林大輔、垣野内景、松村香楠、挾間章博
    • Organizer
      細胞統合生理日本生理学会大会
    • Year and Date
      20120000
  • [Presentation] リソソーム安定化機構と細胞死2011

    • Author(s)
      挾間章博, 三宅将生
    • Organizer
      福島医大・医・細胞統合生理日本生理学会大会
    • Year and Date
      20110000
  • [Presentation] アンフォテリシンB誘導性ネクローシスにおける細胞骨格の役割2010

    • Author(s)
      三宅将生、挾間章博
    • Organizer
      福島医大・医・細胞統合生理日本生理学会大会
    • Year and Date
      20100000

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Published: 2013-07-31   Modified: 2014-09-08  

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