2010 Fiscal Year Final Research Report
Quantitative analyses of G protein activation by the single molecule imaging
| Project/Area Number |
20590224
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | National Institute for Physiological Sciences |
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Principal Investigator |
TATEYAMA Michihiro National Institute for Physiological Sciences, 分子生理研究系, 准教授 (30276472)
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| Co-Investigator(Renkei-kenkyūsha) |
KUBO Yoshihiro 生理学研究所, 分子生理研究系, 教授 (80211887)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 分子・細胞生理学 / FRET |
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| Research Abstract |
G protein coupled receptors (GPCR) play important roles in the inter-cellular signaling and their initial signaling processes were investigated by FRET. FRET values between the fluorescent proteins tethered to the receptors and subunits of GPCRs were measured in living cells. As for the family A Gq coupled receptors, agonist-induced receptor activation, the coupling between receptor and G proteins and the activation of G proteins were detected as ligand-induced FRET changes.
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