2010 Fiscal Year Final Research Report
Direct injection of neuronal nitric oxide synthase into the nucleus tractus soitarii in rats and development of protein therapy
| Project/Area Number |
20590236
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
|
|---|
| Research Institution | Wakayama Medical University |
|---|
Principal Investigator |
MAEDA Masanobu Wakayama Medical University, 医学部, 教授 (80181593)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
WAKI Hidefumi 和歌山県立医科大学, 医学部, 講師 (50274957)
KOHSAKA Akira 和歌山県立医科大学, 医学部, 講師 (00458051)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Keywords | 循環調節 / 孤束核 / 一酸化窒素合成酵素 / タンパク質導入 / タンパク質治療 / 心臓血管中枢 / 血圧 / 延髄 |
|---|
| Research Abstract |
There are some disadvantages in gene therapy. First, several days were required for protein expression after gene transfection. Second, expression of the protein cannot be controlled. On the other hand, in the protein therapy, the biological active transducted protein may have function soon. Expression of the protein can be controlled. Efficient delivery of proteins into the cells in vivo could be achieved only when the molecules are very small. It was shown that intraperitoneal injection of the β-galactosidase (β-gal) protein, fused to the protein transduction domain from the human immunodeficiency virus TAT protein, results in delivery of the biologically active fusion protein to all tissues in mice, including the brain. This technique might be complex. There were no reports that protein transduction into the specific restricted brain area has succeeded. We demonstrated that in vivo nNOS protein direct transduction into the nucleus tractus solitarii (NTS) could change blood pressure. The in vivo delivery of biologically active proteins is a powerful therapeutic tool and the successful transduction of proteins to specific disease-bearing CNS regions would represent a remarkable clinical advance.
|
-
-
-
-
-
-
-
-
-
-
[Journal Article] Sema4D/CD100 deficiency leads to superior performance in mouse motor behavior.2009
Author(s)
Yukawa K, Tanaka T, Takeuchi N, Iso H, Li L, Kohsaka A, Waki H, Miyajima M, Maeda M, Kikutani H, Kumanogoh A
-
Journal Title
Can J Neurol Sci. 36(*)
Pages: 349-355
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Local disturbance of blood flow in the NTS evoked hypertension in rats.2010
Author(s)
Waki H., Bhuyan.M.E.R., Gouraud S.S, Takagishi M., Kohsaka A., Paton J.F.R., Maeda M.
Organizer
Neuro 10 Joint Coference of The 33rd Annual Meeting of the Japan Neuroscience Society, The 53rd Annual Meeting of the Japanese Society for Neurochemistry, and The 20th Annual Meeting of the Japanese Neural Network Society.
Place of Presentation
神戸
Year and Date
2010-09-02
-
[Presentation] Direct transduction of vascular endothelial growth factor into cardiomyocytes reduced infarcted areas in acute myocardial infarction rats.2010
Author(s)
Kaneko M., Hatada A., Nishimura Y., Uchita S., Tuguchi K., Honda K., Nakai T., Waki H., Kohsaka A., Okamura Y., Maeda M.
Organizer
The 87th Annual Meeting of the Physiological Society of Japan
Place of Presentation
岩手
Year and Date
2010-05-21
-
-
-
-
-
-
-
-
[Presentation] Prokineticin 2 as a key molecule that regulates the cardiovascular rhythm in rats.2009
Author(s)
Kohsaka A, Bhuiyan MER, Waki H, Takagishi M, Gouraud S, Cui H, Arakawa H, Negri L, Maeda M
Organizer
The 6th Congress of Asian Sleep Research Society The 34th Annual Meeting of Japanese Society of Sleep Research The 16th Annual Meeting of Japanese Society for Chronobiology
Place of Presentation
Joint Congress. Osaka, Japan(Selected Poster,優秀ポスター講演)
Year and Date
20091024-20091027
-
-
-
-
-
-
-
-
[Presentation] Administration of adrenomedullin 2 in the nucleus tractus solitarius regulates arterial pressure and heart rate in rats.2009
Author(s)
Cui H, Kohsaka A, Waki H, Gouraud SS, Nakamura T, Yukawa K, Hatada A,Yamazaki T, Bhuiyan MER, Takagishi M, Maeda M
Organizer
36th International Congress of Physiological Sciences (IUPS2009)
Place of Presentation
Kyoto
Year and Date
20090727-20090801
-
-
-
[Presentation] Sema4d/cd100 deficiency leads to superior performance in mouse motor behavior.2009
Author(s)
Yukawa K, Tanaka T, Takeuchi N, Iso H, Li L, Kohsaka A, Waki H, Miyajima M, 前田正信, Kikutani H, Kumanogoh A
Organizer
36th International Congress of Physiological Sciences (IUPS2009)
Place of Presentation
Kyoto
Year and Date
20090727-20090801
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] 臓器虚血を伴った急性大動脈解離の検討2008
Author(s)
金子政弘,和気秀文,向阪彰,畑田充俊,Sabine Sandra Stephanie Gouraud-Waki,崔鶴,山崎寿也,Mohammad Eliusur Rahman Bhuiyan,高岸美和,前田正信
Organizer
第101回近畿生理学談話会
Place of Presentation
吹田
Year and Date
2008-09-13
-
-
-
-
-
[Presentation] Protein therapy by direct protein transduction into the brain. Symposium-Approach to whole body : New stream to study autonomic nervous and neuroendocrine system.2008
Author(s)
Maeda M., Waki H., Kohsaka A., Yukawa K., Nakamura T., Kouda K., Owada-Makabe K., Tsubota Y., Gouraud S., Cui H., Yamazaki T., Takagishi M., Bhuiyan E., Hatada A.
Organizer
The 85th Annual Meeting of the Physiological Society of Japan
Place of Presentation
Tokyo
Year and Date
2008-04-25
-
-
-
-
-
-
-
-
-
-
-
-
-
