Molecular mechanism of neural crest cell proliferation, differentiation and death

2010 Fiscal Year Final Research Report

• Project/Area Number: 20590303
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: Chiba University
• Principal Investigator: HATANO Masahiko Chiba University, 大学院・医学研究院, 教授 (20208523)
• Co-Investigator(Kenkyū-buntansha): FUJIMURA Lisa 千葉大学, バイオメディカル研究センター, 助教 (30376363)
• Project Period (FY): 2008 – 2010
• Keywords: 神経堤細胞 / 転写抑制因子 / 腸管神経 / ノックアウトマウス / 細胞周期

Research Abstract

Neural crest cells differentiate into diverse tissue types such as enteric neurons, sympathetic neurons, and melanocytes. Abnormal development of neural crest cells results in Hirschsprung's disease, neuroblastoma, and other anomaly syndrome in newborn. We have identified Ncx which is specifically expressed in neural crest derived cells and searched for its target genes. We found that Nczf, one of the target genes, controls cell proliferation during embryogenesis by negatively regulating p27 expression. Another target gene, Prickle 2, regulates neurite extention in neuroblastoma cell line. Analysis of Ncx target gene network will contribute to the development of new therapeutic methods of neurocristopathy by degenerative medicine using ES or iPS cells.

Research Products

• [Journal Article] CXCR4 expression on activated B cells is downregulated by CD63 and IL-21. (2011)
  • Author(s): Yoshida N., Kitayama D., Arima M., Sakamoto A., Inamine A., Takano H., Hatano M., Koike T., Tokuhisa T.
  • Journal Title: J.Immunol. 186 Pages: 2800-2808
  • Peer Reviewed
• [Journal Article] Bcl6 is required for the development of mouse CD4+ and CD8α+ dendritic cells. (2011)
  • Author(s): Ohtsuka H., Sakamoto A., Pan J., Inage S., Horigome S., Ichii H., Arima M., Hatano M., Okada S., Tokuhisa T.
  • Journal Title: J.Immunol. 186 Pages: 255-263
  • Peer Reviewed
• [Journal Article] The protooncogene BCL6 promotes survival of olfactory sensory neurons. (2010)
  • Author(s): Otaki JM., Hatano M., Matayoshi R., Tokuhisa T., Yamamoto H.
  • Journal Title: Dev.Neurobiol. 70 Pages: 424-435
  • Peer Reviewed
• [Journal Article] A kelch family protein Nd1-L functions as a metastasis suppressor in cancer cells via Rho family proteins mediated mechanism. (2009)
  • Author(s): Ohta Y., Fujimura L., Nishio S., Arima M., Sakamoto A., Shimada H., Ochiai T., Tokuhisa T., Hatano M.
  • Journal Title: Int.J.Oncol. 36 Pages: 427-434
  • Peer Reviewed
• [Journal Article] Prickle promotes neurite outgrowth via the Dishevelled dependent pathway in C1300 cells. (2009)
  • Author(s): Fujimura L., Takano H., Sato Y., Tokuhisa T., Hatano M.
  • Journal Title: Neuroscience Letters 467 Pages: 6-10
  • Peer Reviewed
• [Journal Article] IL-23 and Th17 cells enhance Th2 cellmediated eosinophilic airway inflammation in mice. (2008)
  • Author(s): Wakashin H., Hirose K., Maezawa Y., Kagami S-I., Suto A., Watanabe N., Saito Y., Hatano M., Tokuhisa T., Iwakura Y., Puccetti P., Iwamoto I., Nakajima H.
  • Journal Title: Am.J.Resp.Crit.Care Med. 178 Pages: 1023-1032
  • Peer Reviewed
• [Journal Article] A lysosomal protein negatively regulates surface T cell antigen receptor expression by promoting CD3ζ degradation. (2008)
  • Author(s): Ouchida R., Yamasaki S., Hikida M., Masuda K., Kawamura K., Wada A., Mochizuki S., Tagawa M., Sakamoto A., Hatano M., Tokuhisa T., Koseki H., Saito T., Kurosaki T., Wang Ji-Y.
  • Journal Title: Immunity 29 Pages: 33-43
  • Peer Reviewed
• [Journal Article] A distinct role for IL-4 and IL-21 in their collaboration on proliferation and differentiation of activated B ells. (2008)
  • Author(s): Saito T., Kitayama D., Sakamoto A., Tsuruoka N., Arima M., Hatano M., Miyazaki M., Tokuhisa T.
  • Journal Title: Immunobiol. 213 Pages: 545-555
  • Peer Reviewed
• [Journal Article] A role for Bcl6 in sequential class switch recombination to IgE in B cells stimulated with IL-4 and IL-1.Mol. (2008)
  • Author(s): Kitayama D., Sakamoto A., Arima M., Hatano M., Miyazaki M., Tokuhisa T.
  • Journal Title: Immunol. 45 Pages: 1337-1345
  • Peer Reviewed
• [Presentation] Ras-ERKカスケードのアンタゴニストDA-Rafは肺形成を制御している (2010)
  • Author(s): 渡邊-高野晴子、横山隆志, 幡野雅彦, 徳久剛史, 高野和儀、遠藤剛
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] IL-21 is essential for long-lived plasma cell differentiation. (2010)
  • Author(s): Inamine A., Sakamoto A., Yoshida N., Arima M., Ikari J., Hatano M., Okamoto Y., Tokuhisa T.
  • Organizer: 14th International congress of Immunology
  • Place of Presentation: Kobe
  • Year and Date: 2010-08-26
• [Presentation] A critical role of the IL-4 intron enhancer in chromatin remodeling of Th2 cytokine gene loci. (2010)
  • Author(s): Arima M.,Ogasawara T., Fujimura L., Sakamoto A., Hatano M., Tokuhisa T.
  • Organizer: 14th International congress of Immunology
  • Place of Presentation: Kobe
  • Year and Date: 2010-08-25
• [Presentation] A role of Bcl6 in differentiation of memory precursor CD8+ T cells. (2010)
  • Author(s): Pan J., Sakamoto A., Yamashita K., Kouno M., Arima M., Hatano M., Tokuhisa T.
  • Organizer: 14th International congress of Immunology
  • Place of Presentation: Kobe
  • Year and Date: 2010-08-25
• [Presentation] Role of Bcl6 in the generation and maintenance of follicular helper T cells. (2010)
  • Author(s): Sakamoto A., Pan J., Kohno M., Arima M., Hatano M., Tokuhisa T.
  • Organizer: 14th International congress of Immunology
  • Place of Presentation: Kobe
  • Year and Date: 2010-08-25
• [Presentation] IL-4 and IL21 inversely regulate CXCR4 expression on activated B cells. (2010)
  • Author(s): Yoshida N., Sakamota A., Kitayama D., Arima M., Inamine A., Hatano M., Koike T., Tokuhisa T.
  • Organizer: 14th International congress of Immunology
  • Place of Presentation: Kobe
  • Year and Date: 2010-08-24
• [Presentation] The role of PHF11 in activation of murine B cells. (2010)
  • Author(s): Ikari J., Fujimura L., Sakamoto A., Hatano M., Tatsumi K., Tokuhisa T., Arima M.
  • Organizer: 14th International congress of Immunology
  • Place of Presentation: Kobe
  • Year and Date: 2010-08-24
• [Presentation] Krab-zinc finger蛋白Nczfの個体発生における機能解析 (2009)
  • Author(s): 長谷川勇太、小関千紗、寺竹洋一、藤村理紗、徳久剛史, 幡野雅彦
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] 神経突起伸長における細胞内平面極性タンパクPrickleの機能解析 (2008)
  • Author(s): 藤村理紗、高野晴子, 坂本明美, 有馬雅史、徳久剛史, 幡野雅彦
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Book] Role of Prickle1 and Prickle2 in neurite outgrowth in murine neuroblastoma cells. Panar cell polarity methods and protocols.in "Methods in Molecular Biology"(Edited by Chen P., Turksen K.)
  • Author(s): Fujimura L., Hatano M.
  • Publisher: Springer, In Press
• [Remarks] ホームページ等
  • URL: http://www.m.chibau.ac.jp/class/biomedical/