2010 Fiscal Year Final Research Report
Analyses of Werner helicase functions : From the viewpoint of its telomere stabilization
| Project/Area Number |
20590389
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Tokyo Metropolitan Institute of Gerontology |
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Principal Investigator |
ISHIKAWA Naoshi Tokyo Metropolitan Institute of Gerontology, 研究員 (30184485)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA KenーIchi 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 研究員 (60159069)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKUBO Kaiyo 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 研究部長 (00154956)
NAKAMURA Ken-Ichi 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 研究員 (60159069)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 老化 / がん化 / ウェルナー症候群 / テロメア |
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| Research Abstract |
we measured terminal restriction fragment (TRF) lengths of 10 skin samples collected from extremities of 8 WS patients aged between 30 and 61 years that had been surgically amputated because of skin ulceration. Whereas the values of TRF length in younger WS patients (in their thirties) were within the normal range, those in older WS patients were markedly shorter relative to non-WS controls. Regression analyses indicated that the TRF length in WS was significantly shorter than that in controls (p <0.001). Furthermore, we found that TRF lengths in muscle adjacent to the examined epidermis were also significantly shorter than those of controls (p = 0.047). These data demonstrate for the first time that in vivo telomere loss is accelerated in systemic organs of WS patients, suggesting that abnormal telomere erosion is one of the major causes of early onset of age-related symptoms and a predisposition to sarcoma and carcinoma in WS.
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[Journal Article] Accelerated in vivo epidermal telomere loss in Werner syndrome.2011
Author(s)
Ishikawa N, Nakamura K, Izumiyama-Shimomura N, Aida J, Ishii A, Goto M, Ishikawa Y, Asaka R, Matsuura M, Hatamochi A, Kuroiwa M, Takubo K.
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Journal Title
Peer Reviewed
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[Journal Article] Alcoholics show reduced telomere length in the oesophagus.2011
Author(s)
Aida J, Yokoyama A, Izumiyama-Shimomura N, Nakamura K, Ishikawa N, Poon SSS, Fujiwara M, Sawabe M, Matsuura M, Arai T, Takubo K.
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Journal Title
Journal of Pathology. 223
Pages: 410-416
Peer Reviewed
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[Journal Article] Chromosomal instability and telomere lengths of each chromosomal arm measured by Q-FISH in human fibroblast strains prior to replicative senescence.2010
Author(s)
Takubo K, Aida J, Izumiyama N, Ishikawa N , Fujiwara M,. Poon SSS, Kondo H, Kammori M, Matsuura M, Sawabe M, Arai T, Baird DM, Nakamura K.
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Journal Title
Mechanisms of Ageing and Development. 131
Pages: 614-624
Peer Reviewed
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[Journal Article] Telomere lengths in the oral epithelia with and without carcinoma.2010
Author(s)
Aida J, Izumo T, Shimomura N, Nakamura K, Ishikawa N, Matsuura M, Poon SS, Fujiwara M, Sawabe M, Arai T, Takubo K.
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Journal Title
Europian Journal of Cancer. 46
Pages: 430-438
Peer Reviewed
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[Journal Article] Q-FISH analysis of telomere and chromosome instability in theoesophagus with and without squamous cell carcinoma in situ.2010
Author(s)
Takubo K, Fujita M , Izumiyama N, Nakamura K, Ishikawa N, Poon SS, Fujiwara M, Sawabe M, Matsuura M, Grabsch H, Arai T, Aida J.
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Journal Title
Journal of Pathology. 221
Pages: 201-209
Peer Reviewed
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