How P.vivax parasite maintains multiple infection in areas of low intensity malaria?

2010 Fiscal Year Final Research Report

• Project/Area Number: 20590422
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Osaka University
• Principal Investigator: HORII Toshihiro Osaka University, 微生物病研究所, 教授 (80142305)
• Co-Investigator(Kenkyū-buntansha): ARISUE Nobuko 大阪大学, 微生物病研究所, 助教 (00242339) ; ISHII Ken 大阪大学, 微生物病研究所, 准教授 (00448086) ; TOUGAN Takahiro 大阪大学, 微生物病研究所, 助教 (20379093) ; YAGI Masanori 大阪大学, 微生物病研究所, 特任研究員 (60452463) ; TAI Kumiko 大阪大学, 微生物病研究所, 教務職員 (00187907)
• Co-Investigator(Renkei-kenkyūsha): TANABE Kazuyuki 大阪大学, 微生物病研究所, 招聘教授 (40047410)
• Research Collaborator: PALACPAC Nirianne M. Q. 一般財団法人阪大微生物病研究会 ; COBAN Cevayir 大阪大学, 免疫学フロンティア研究センター ; ZEYREK Fadile Yildiz Harran University Medical Faculty, Turkey ; UDOMSANGPETCH Rachanee Mahidol University, Thailand ; SATTABONGKOT Jetsumon USAMC Armed Forces Research Institute of Medical Sciences, Thailand ; CULLETON Richard 長崎大学, 熱帯医学研究所
• Project Period (FY): 2008 – 2010
• Keywords: 三日熱マラリア原虫 / 遺伝子多型 / 多重感染 / 流行度 / 組み換え

Research Abstract

In P.vivaxpatients, multiple infection is common even in low infection intensity areas. This study was conducted to understand the paradox. Haplotype diversities and the number of allele of the Plasmodium vivaxpopulation isolated from Thailand, Turkey and Africa were analyzed using two antigen coding genes (msp1, sera5) and four microsatellite makers (2.21, 3.35, AT, GT). All parasite samples showed multiple numbers of alleles and high degree of haplotype diversities against each locus. The nucleotide diversity of Group IV sera genes of P.vivax were 200 times higher than those of P.falciparum, implying possible immune pressure from the host. The multiple infection of P.vivax seemed to be caused by the divergent parasite population and it might be result in the positive selection.

Research Products

• [Presentation] マラリア原虫SERA 遺伝子ファミリーの系統特異的な進化 (2010)
  • Author(s): 有末伸子、Nirianne M.Q.Palacpac、川合覚、平井誠、田邉和裄、堀井俊宏
  • Organizer: 第9回分子寄生虫・マラリア研究フォーラム
  • Place of Presentation: 長崎
  • Year and Date: 2010-10-09
• [Presentation] The SERA gene family from several Plasmodium species : Picking important clues for the evolutionary puzzle of this gene family. (2010)
  • Author(s): Nobuko Arisue, Nirianne M.Q.Palacpac, Satoru Kawai, Makoto Hirai, Kazuyuki Tanabe, Toshihiro Horii.
  • Organizer: The 10th Awaji International Forum on Infection and Immunity
  • Place of Presentation: 淡路島
  • Year and Date: 2010-09-09
• [Presentation] マラリア原虫sera遺伝子ファミリーの多型解析 (2010)
  • Author(s): 有末伸子、Nirianne M.Q.Palacpac、田邊和裄、堀井俊宏
  • Organizer: 第79回日本寄生虫学会大会
  • Place of Presentation: 旭川
  • Year and Date: 2010-05-20
• [Presentation] Comparative analysis of human vivax malaria infections in different endemic area (2009)
  • Author(s): Nirianne M.Q.Palacpac, Nobuko Arisue, Kazuyuki Tanabe, Hiromi Sawai, Richard Culleton Rachanee Udomsangpetch, Jetsumon Sattabongkot, Fadile Zeyrek, Cevayir Coban, Toshihiro Horii.
  • Organizer: International Joint Forum on Infectious Disease 2009
  • Place of Presentation: Bangkok
  • Year and Date: 2009-09-16
• [Presentation] The SERA gene family : Impotant clues from several Plasmodium species. (2009)
  • Author(s): Nobuko Arisue, Nirianne M.Q.Palacpac, Satoru Kawai, Makoto Hirai, Kazuyuki Tanabe, Toshihiro Horii.
  • Organizer: International Joint Forum on Infectious Disease 2009
  • Place of Presentation: Bangkok
  • Year and Date: 2009-09-16
• [Presentation] Diversi ty in geographically distinct Plasmodium vivax analysis of human vivax malaria infections in different endemic area (2009)
  • Author(s): Nirianne M.Q.Palacpac, Nobuko Arisue, Richard Culleton, Kazuyuki Tanabe, Fadile Yildiz Zeyrek, Cevayir Coban, Jetsumon Sattabongkot, Takafumi Tsuboi, Motmi, Torii, Rachanee Udomsangpetch, Toshihiro Horii.
  • Organizer: International Joint Forum on Infectious Disease 2009
  • Place of Presentation: Bangkok
  • Year and Date: 2009-09-16
• [Presentation] The SERA gene family : Impotant clues from several Plasmodium species (2009)
  • Author(s): Nobuko Arisue, Nirianne M.Q.Palacpac, Satoru Kawai, Makoto Hirai, Kazuyuki Tanabe, Toshihiro Horii.
  • Organizer: International Joint Forum on Infectious Disease 2009
  • Place of Presentation: Bangkok
  • Year and Date: 2009-09-16
• [Presentation] Comparative analysis of human vivax malaria infections in different endemic area (2009)
  • Author(s): Nirianne M.Q.Palacpac, Nobuko Arisue, Kazuyuki Tanabe, Hiromi Sawai, Richard Culleton, Rachanee Udomsangpetch, Jetsumon Sattabongkot, Fadile Zeyrek, Cevayir Coban, Toshihiro Horii.
  • Organizer: The 9th AWAJI International Forum on Infection and Immunity
  • Place of Presentation: 淡路島
  • Year and Date: 2009-09-10
• [Presentation] The SERA gene family : Impotant clues from several Plasmodium species (2009)
  • Author(s): Nobuko Arisue, Nirianne M. Q. Palacpac, Satoru Kawai, Makoto Hirai, Kazuyuki Tanabe, Toshihiro Horii.
  • Organizer: The 9th AWAJI International Forum on Infection and Immunity
  • Place of Presentation: 淡路島
  • Year and Date: 2009-09-10
• [Presentation] Diversity in geographically distinct Plasmodium vivax populations (2008)
  • Author(s): Nirianne M.Q.Palacpac, Nobuko Arisue, Richard Culleton, Kazuyuki Tanabe, Fadile Yildiz Zeyrek, Cevayir Coban, Jetsumon Sattabongkot, Takafumi Tsuboi, Motmi, Torii, Rachanee Udomsangpetch, Toshihiro Horii.
  • Organizer: 第77回日本寄生虫学会大会
  • Place of Presentation: 長崎
  • Year and Date: 2008-04-04