Analyses and applications of novel mechanism of phospho-independent regulation of lipid metabolism by Trypanosoma brucei 14-3-3

2010 Fiscal Year Final Research Report

• Project/Area Number: 20590434
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Kurume University
• Principal Investigator: INOUE Masahiro Kurume University, 医学部, 教授 (00232562)
• Project Period (FY): 2008 – 2010
• Keywords: 脂質代謝 / 蛋白リン酸化酵素 / 結合蛋白 / ブルーストリパノソーマ / 2014 / 03 / 03

Research Abstract

Trypanosoma brucei is a causative agent of sleeping sickness. More than 30,000 infected patients died of this devastating disease. One of the aims for this research is to identify the molecular mechanisms by which T. brucei 14-3-3s control the motility, cytokinesis, and cell cycle focusing on the lipid metabolism controlled by Tb14-3-3s as we discovered. We found that Tb14-3-3s interact with the enzymes involved in sugar or lipid metabolism, and protein kinases. One of the protein kinases among them is found out to be critical for the regulation of the motility, cytokinesis and cell cycle.

Research Products

• [Journal Article] Phosphorylation- Dependent Protein Interaction with Trypanosoma brucei 14-3-3 Proteins that Display Atypical Target Recognition. (2010)
  • Author(s): Inoue M., et al.
  • Journal Title: PLoS One 5(12) Pages: e15566
  • Peer Reviewed
• [Journal Article] Epistatic and Functional Interactions of Catecho-O-Methyltransferase(COMT) and AKT1 on Neuregulin 1-ErbB Signaling in Cell Models. (2010)
  • Author(s): Sei Y., et al.
  • Journal Title: PLoS One 5(5) Pages: e10789
  • Peer Reviewed
• [Journal Article] Phosphorylation-dependent Lys63-linked polyubiquitination of Daxx is essential for sustained TNF -{alpha}-induced ASK1 activation. (2009)
  • Author(s): Fukuyo Y., Kitamura T., Inoue M., Horikoshi NT, Higashikubo R, Hunt CR, Usheva A, Horikoshi N.
  • Journal Title: Cancer Res. 69(19) Pages: 7512-7517
  • Peer Reviewed
• [Journal Article] Mutant p53 disrupts the stress MAPK activation circuit induced by ASK1-dependent stabilization of Daxx. (2009)
  • Author(s): Kitamura T., Fukuyo Y., Inoue M., Horikoshi NT., Shindoh, M., Rogers BE., Usheva, A., Horikoshi, N.
  • Journal Title: Cancer Res. 69(19) Pages: 7681-7688
  • Peer Reviewed
• [Journal Article] A Critical Role for highly Conserved GLU610 Residue of Oligopeptidase B from Trypanosoma Brucei in Thermal Stability. (2009)
  • Author(s): Ismail NI., Yuasa T., Yuasa K., Nambu Y, Nisimoto M., Goto M., Matsuki H., Inoue M., Nagahama M., Tsuji A.
  • Journal Title: J.Biochem. 147(2) Pages: 201-211
  • Peer Reviewed
• [Presentation] Biological significance of heterodimeric form of Trypanosoma brucei 14-3-3I and II. (2009)
  • Author(s): 井上雅広、安田幸一、上村春樹、杉本佳織、井上大志、福間利英
  • Organizer: 第78回日本寄生虫学会大会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-27
• [Presentation] Biological significance of heterodimeric form of Trypanosoma brucei 14-3-3I and II. (2009)
  • Author(s): 井上雅広
  • Organizer: 日米医学協力研究会 寄生虫疾患専門部会 第43回日米合同会議
  • Place of Presentation: 東京
  • Year and Date: 2009-01-08
• [Presentation] 普遍的でない分子認識機構をもつトリパノソーマ14-3-3 に結合するキナーゼの発見 (2008)
  • Author(s): 井上雅広
  • Organizer: 第9回分子寄生虫・マラリア研究フォーラム
  • Place of Presentation: 長崎
  • Year and Date: 2008-10-09
• [Patent(Industrial Property Rights)] 原虫の増殖阻害剤を検出する方法 (2008)
  • Inventor(s): 井上雅広
  • Industrial Property Rights Holder: 井上雅広、上村春樹
  • Industrial Property Number: 特許,3922710X08
  • Filing Date: 2008-10-08