2010 Fiscal Year Final Research Report
Translational research on a drug delivery system using substrate binding domain derived from bacterial collagenases
Project/Area Number |
20590452
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Bacteriology (including Mycology)
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Research Institution | Kitasato University |
Principal Investigator |
MATSUSHITA Osamu Kitasato University, 医学部, 教授 (00209537)
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Co-Investigator(Kenkyū-buntansha) |
KOIDE Takaki 早稲田大学, 先進理工学部, 教授 (70322253)
TAMAI Eiji 松山大学, 薬学部, 准教授 (40333512)
MIYATA Shigeru 香川大学, 医学部, 講師 (90314913)
|
Co-Investigator(Renkei-kenkyūsha) |
MIMAMI Junzaburo 香川県立保健医療大学, 保健医療学部, 教授 (40157566)
|
Project Period (FY) |
2008 – 2010
|
Keywords | ガス壊疽菌群 / 毒素 / 構造活性相関 / コラーゲン結合ドメイン / 成長因子 / 骨新生 / 鼓膜閉鎖術 / 人工皮膚 |
Research Abstract |
'Flesh-eating bacteria' produce collagenases to hydrolyze collagen fibrils in tissue. These enzymes possess domain(s) to bind to this insoluble substrate (CBD). We identified amino acid residues responsible for the binding. We also showed that CBD binds preferentially to the under-wound region in the collagen molecule, and that the binding is unidirectional. Ca^<2+> binds to the linker region of CBD. We showed that the binding modifies CBD into a more stable and more efficient conformation. Finally, we showed the potential benefits of matrix anchoring of various bioactivity substances using CBD, which are applicable to various therapies in regenerative medicine.
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[Journal Article] Monthly administration of a novel PTH-vollagen binding domain fusion protein is anabolic in mice. Calcif.2011
Author(s)
Ponnapakkam T., Katikaneni, R., Miller, E., Ponnapakkam, A., Suda, H., Miyata, S., Suva, L.J., JSakon, J., Matsushita, O., Gensure, R.C.
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Journal Title
Peer Reviewed
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[Journal Article] Development of a high-throughput screening system for the compounds that inhibit collagen-protein interactions.2009
Author(s)
Okano-Kosugi, H., Matsushita, O., Asada, S., Herr, A.B., Kitagawa, K., Koide, T.
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Journal Title
Anal.Biochem. 394
Pages: 125-131
Peer Reviewed
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[Journal Article] Unidirectional binding of clostridial collagenase to triple helical substrates.2009
Author(s)
Philominathan, S.T., Koide, T., Hamada, K., Yasui, H., Seifert, S., Matsushita, O., Sakon, J.
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Journal Title
J.Biol.Chem. 284
Pages: 10868-10876
Peer Reviewed
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[Journal Article] High-level expression of his-tagged clostridial collagenase in Clostridium perfringens.2008
Author(s)
Tamai, E., Miyata, S., Tanaka, H., Nariya, H., Suzuki,M., Matsushita, O., Hatano, N., Okabe, A.
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Journal Title
Appl.Microbiol.Biotechnol. 80
Pages: 627-635
Peer Reviewed
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[Presentation] From bacterial toxin to drug.2010
Author(s)
Matsushita O., Collagen anchoring
Organizer
11th Joint Symposium Robert Koch Institute and The Kitasato Institute Kitasato University
Place of Presentation
東京都港区
Year and Date
2010-10-26
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