2010 Fiscal Year Final Research Report
Regulation of SARS-CoV replication by cyclophilin
| Project/Area Number |
20590467
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Juntendo University |
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Principal Investigator |
YAMAMOTO Norio Juntendo University, 医学部, 助教 (40323703)
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| Project Period (FY) |
2008 – 2010
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| Keywords | SARSコロナウイルス / サイクロスポリンA |
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| Research Abstract |
To investigate the relationship between cyclophilin and SARS-CoV replication, we introduced shRNA expression vectors to cells and compare the virus titer produced from these cells. Efficiency of SARS-CoV replication was much lower in the cells expressing shRNA against cyclophilin A and B than in the cells expressing control shRNA. The results of time-of-addition assay and entry assay showed that cyclosporin A inhibited SARS-CoV replication at the post-entry step in viral life cycle. These results suggested that cyclophilins could play a role as positive regulators of SARS-CoV replication at the post-entry step.
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