2010 Fiscal Year Final Research Report
Proteomic analysis of thrombopoietin signaling
| Project/Area Number |
20590544
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied pharmacology
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| Research Institution | Osaka City University |
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Principal Investigator |
NAKAO Takafumi Osaka City University, 大学院・医学研究科, 講師 (50326261)
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| Project Period (FY) |
2008 – 2010
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| Keywords | ファーマコゲノミクス / プロテオーム / 細胞内シグナル伝達 |
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| Research Abstract |
Thrombopietin is the most important cytokine for thrombopoiesis. The importance of thrombopoietin signaling is immediately apparent; morbidity and mortality from bleeding due to moderate to severe thrombocytopenia is a major problem of a wide range of patients. The aim of this study is to find the molecules that interact with Akt, serine-threonine kinase which plays a crucial role for megakaryocytopoiesis, in human hematopoietic cells. To this end, we established the affinity purification and proteomic analysis to elucidate the role of Akt signaling in megakaryocytopoiesis. However, we could not detect Akt-binding protein under thrombopoietin-dependent manner.
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