2010 Fiscal Year Final Research Report
Development of a new diagnostic system of inflammatory diseases by measuring S100A8/A9・cytokine complexes
| Project/Area Number |
20590567
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Kyoto University |
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Principal Investigator |
IKEMOTO Masaki Kyoto University, 大学院・医学研究科, 准教授 (80144385)
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| Co-Investigator(Kenkyū-buntansha) |
UEMOTO Shinji 京都大学, 大学院・医学研究科, 教授 (40252449)
EGAWA Haruto 朝日大学, 歯学部, 教授 (40293865)
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| Project Period (FY) |
2008 – 2010
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| Keywords | S100蛋白 / マクロファージ / 好中球 / 拒絶反応 / 急性炎症 / サイトカイン |
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| Research Abstract |
We found that S100A8/A9 non-specifically bind with fibronectin (h-FN), and that both the two protein exist on surface membrane of neutrophils and monocytes. The h-FN could be conclusively transported with S100A8/A9 in blood and/or on immunological cells, and effectively prevent further attack by various internal oxidants or repair damaged liver tissue in vivo. When recombinant human S100A8 was intravenously injected in the rats with LPS-induced liver damage, not only expression of r-S100A9 but also the activities of AST, ALT and LD significantly went down in comparison with those of the rats with liver damage, strongly suggested that the r-S100A8 could serve as a regulator of acute inflammatory reaction in the rats with LPS-induced damage. Immunohistochemistry provided interesting microscopic images, in which much r-S100A8 and r-S100A9 were observed in many macrophages in the heart tissues of rats with experimental autoimmune myocarditis. In addition, macrophages were positive for not only CD68 antigen but also r-S100A8 and/or r-S100A9 proteins, strongly suggesting active condition of the macrophages in inflamed areas of the heart tissues with EAM. Therefore, r-S100A8 and r-S100A9 in macrophages should be novel indexes for active condition of the cells in the EAM rats.
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[Journal Article] Identification of serum proteins that bind with S100A8, S100A9 and S100A8/A9 : clinical significance of using proteins for monitoring the postoperative condition of liver recipients.2010
Author(s)
Namura T, Arai S, Okawa K, Koike A, Yamada S, Saita N, Nagae A, Itoh H, Totani M, Uemoto S, Ikemoto M.
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Journal Title
Clin Chim Acta Vol.411
Pages: 1766-1773
Peer Reviewed
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[Journal Article] Enhanced Expression of the S100A8/A9 Complex in AcuteMyocardial Infraction Patients.2010
Author(s)
Katashima T, Naruko T, Terasaki F, Fujita M, Otsuka K, Murakami S, Sato S, Hiroe M, Ikura Y, Ueda M, Ikemoto M, Kitaura Y.
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Journal Title
Circ J Vol.74
Pages: 741-748
Peer Reviewed
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[Journal Article] Suppression of inflammation in rat autoimmunemyocarditis by S100A8/A9 through modulation ofthe proinflammatory cytokine network.2009
Author(s)
Otsuka K, Terasaki F, Ikemoto M, Fujita M, Tsukada B, Takashima T, Kanzaki Y, Sohmiya K,Kono T, Toko H, Fujita M, Kitaura Y l.
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Journal Title
Eur J of Heart Failure Vol.10
Pages: 1093-1101
Peer Reviewed
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