2010 Fiscal Year Final Research Report
Treatment of diabetic neuropathy by DRG-targeting vector
| Project/Area Number |
20590995
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
YASUDA Hitoshi Shiga University of Medical Science, 医学部, 教授 (80135467)
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| Co-Investigator(Kenkyū-buntansha) |
KOJIMA Hideto 滋賀医科大学, 医学部, 准教授 (00225434)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 遺伝子治療 / 糖尿病合併症 / ファージ・ベクター / RT PCR |
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| Research Abstract |
We have tried to make in vivo gene therapy vectors targeting dorsal root ganglia (DRG) by using 3 kinds of 7 amino acids sequence that could specifically bind to DRG neurons (DRG1, DRG2, DRG3). Phage vector system was obtained by transformations of the isolated 3 kinds of peptites to the PIII of the M13 phage. The test vectors were made by an introduction of green fluorescent protein (GFP) to multi-cloning site of the vector to see the effect of the gene expression. The vectors were administrated to subarachnoid space of mice, and the GFP-expression was examined in DRG tissues. Compared with the results of a study using GST-fusion protein, the expression of GFP by a gene transfer was very low by phage vector system. This might be caused by the unstableness of the phage itself, and we need a further arrangement for the improvement of the expression efficiency.
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