2010 Fiscal Year Final Research Report

Treatment of diabetic neuropathy by DRG-targeting vector

Research Project

Project/Area Number	20590995
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Neurology
Research Institution	Shiga University of Medical Science
Principal Investigator	YASUDA Hitoshi Shiga University of Medical Science, 医学部, 教授 (80135467)
Co-Investigator(Kenkyū-buntansha)	KOJIMA Hideto 滋賀医科大学, 医学部, 准教授 (00225434)
Project Period (FY)	2008 – 2010
Keywords	遺伝子治療 / 糖尿病合併症 / ファージ・ベクター / RT PCR
Research Abstract	We have tried to make in vivo gene therapy vectors targeting dorsal root ganglia (DRG) by using 3 kinds of 7 amino acids sequence that could specifically bind to DRG neurons (DRG1, DRG2, DRG3). Phage vector system was obtained by transformations of the isolated 3 kinds of peptites to the PIII of the M13 phage. The test vectors were made by an introduction of green fluorescent protein (GFP) to multi-cloning site of the vector to see the effect of the gene expression. The vectors were administrated to subarachnoid space of mice, and the GFP-expression was examined in DRG tissues. Compared with the results of a study using GST-fusion protein, the expression of GFP by a gene transfer was very low by phage vector system. This might be caused by the unstableness of the phage itself, and we need a further arrangement for the improvement of the expression efficiency.

(2 results)