2010 Fiscal Year Final Research Report

Study for therapeutic effect of hepatocyte specific Pten deficiency on severe obesity.

Research Project

Project/Area Number	20591041
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Metabolomics
Research Institution	Akita University
Principal Investigator	OHSHIMA Shigetoshi Akita University, 医学部, 講師 (50375268)
Co-Investigator(Kenkyū-buntansha)	HORIE Yasuo 秋田大学, 医学部, 講師 (30282164)
Project Period (FY)	2008 – 2010
Keywords	PTEN / 肥満 / 遺伝子治療
Research Abstract	Our aim is to investigate whether hepatocyte-specific Pten deficiency can improve obesity in the leptin-deficient ob/ob mouse by activating lipogenesis in the liver. For this purpose, we have mated hepatocyte-specific Pten deficient mouse with leptin-deficient ob/ob mouse and established ob/ob/AlbCre/Pten^<flox/flox> mouse. ob/ob mice (ob/ob group), ob/ob/AlbCre/Pten^<flox/flox> mice (double KO group), AlbCre/Pten^<flox/flox> mice (KO group), Wild type mice (WT group) were sacrificed at 40 weeks just after weaning and measured body weight, fat tissue weight around testis or ovary, liver weight, triglyceride content in the liver, serum transaminase level and total ROS level in serum. Moreover we investigated Akt activation by western blot analysis and examined the expression of various genes especially related to β-oxidation by quantitative real-time PCR. The result is Hepatocyte-specific Pten deficiency improved obesity in the leptin-deficient ob/ob mouse by activating lipogenesis in the liver.

(2 results)