2010 Fiscal Year Final Research Report
The role of Notch signaling in the leukemia stem cells.
| Project/Area Number |
20591111
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KUMANO Keiki The University of Tokyo, 医学部附属病院, 講師 (90396721)
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| Project Period (FY) |
2008 – 2010
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| Keywords | Notch / 白血病幹細胞 / ニッチ |
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| Research Abstract |
Murine bone marrow cells transformed by MLL-ENL induced AML in the culture for myeloid condition, and ALL in the culture for lymphoid condition. MLL-ENL induced AML cells were transduced with Notch1 active from and cultured on the OP-9 stromal cells. The one part of these cells expressed T cell specific surface marker. In the same way, AML cells trasduced with B cell gene (Pax5, E2A, EBF) expressed B cell marker.
AML cells were cultured on the Delta1 expressing OP-9 cells and activated by the Notch signaling. As a result, T cell differentiation was observed in the part of the AML cells. These results indicated that Multipotent cells existed in the MLL-ENL AML cells and the differentiation potential might be regulated by the niche signaling.
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[Journal Article] Hesl immortalizes committed progenitors and plays a role in blast crisis transition in chronic myelogenous leukemia.2010
Author(s)
Nakahara F, Sakata-Yanagimoto M, Komeno Y, Kato N, Uchida T, Haraguchi K, Kumano K, Harada Y, Harada H, Kitaura J, Ogawa S, Kurokawa M, Kitamura T, Chiba S.
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Journal Title
Blood 115
Pages: 2872-2881
Peer Reviewed
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[Journal Article] Dual antitumor mechanisms of Notch signaling inhibitor in a T-cell acute lymphoblastic leukemia xenograft model.2009
Author(s)
Masuda S, Kumano K, Suzuki T, Tomita T, Iwatsubo T, Natsugari H, Tojo A, Shibutani M, Mitsumori K, Hanazono Y, Ogawa S, Kurokawa M, Chiba S.
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Journal Title
Cancer Sci. 100
Pages: 2444-2450
Peer Reviewed
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[Journal Article] Gain-of-function of mutated C-CBL tumour suppressor in myeloid neoplasms.2009
Author(s)
Sanada M, Suzuki T, Shih LY, Otsu M, Kato M, Yamazaki S, Tamura A, Honda H, Sakata-Yanagimoto M, Kumano K, Oda H, Yamagata T, Takita J, Gotoh N, Nakazaki K, Kawamata N, Onodera M, Nobuyoshi M, Hayashi Y, Harada H, Kurokawa M, Chiba S, Mori H, Ozawa K, Omine M, Hirai H, Nakauchi H, Koeffler HP, Ogawa S.
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Journal Title
Nature. 460
Pages: 904-908
Peer Reviewed
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[Journal Article] Gain-of-function mutations and copy number increases of Notch2 in diffuse large B-cell lymphoma.2009
Author(s)
Lee SY, Kumano K, Nakazaki K, Sanada M, Matsumoto A, Yamamoto G, Nannya Y, Suzuki R, Ota S, Ota Y, Izutsu K, Sakata-Yanagimoto M, Hangaishi A, Yagita H, Fukayama M, Seto M, Kurokawa M, Ogawa S, Chiba S.
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Journal Title
Cancer Sci. 100
Pages: 920-6
Peer Reviewed
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[Journal Article] Notch activation induces the generation of functional NK cells from human cord blood CD34-positive cells devoid of IL 15.2009
Author(s)
Haraguchi K, Suzuki T, Koyama N, Kumano K, Nakahara F, Matsumoto A, Yokoyama Y, Sakata-Yanagimoto M, Masuda S, Takahashi T, Kamijo A, Takahashi K, Takanashi M, Okuyama Y, Yasutomo K, Sakano S, Yagita H, Kurokawa M, Ogawa S, Chiba S.
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Journal Title
J Immunol. 182
Pages: 6168-78
Peer Reviewed
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[Journal Article] Both Notchl and Notch2 contribute to the regulation of melanocyte homeostasis.2008
Author(s)
Kumano K, Masuda S, Sata M, Saito To, Lee SY, Sakata-Yanagimoto M, Tomita T, Iwatsubo T, Natsugari H, Kurokawa M, Ogawa S, Chiba S.
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Journal Title
Pigment Cell Melanoma Res. 21
Pages: 70-8
Peer Reviewed
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