Regulation of hematopoiesis by dendritic cells

2010 Fiscal Year Final Research Report

• Project/Area Number: 20591144
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hematology
• Research Institution: Akita University
• Principal Investigator: SAWADA Kenichi Akita University, 大学院・医学系研究科, 教授 (90226069)
• Co-Investigator(Renkei-kenkyūsha): OHTEKI Toshiaki 東京医科歯科大学, 難治疾患研究所先端分子医学研究部門, 教授 (50233200)
• Project Period (FY): 2008 – 2010
• Keywords: 樹状細胞 / 造血幹細胞 / 自然免疫 / トール様受容体 / 血球貪食症候群

Research Abstract

Dendritic cells (DCs) maintain the homeostasis of immunologic system by regulating lymphocyte functions through the production of various cytokines. The collapse of this regulatory system promotes immunologic disorders such as compromised infection, overresponse to immunologic stimuli and autoimmune diseases. DCs might influence differentiation and proliferation of hematopoietic stem cells, however, a large part has remained unclear. This study, at least in part, revealed the mechanism and the physiological role of DCs phagocytosing self-hematopoietic cells and the influence of microbe-derived nucleic acid such as DNA and RNA.

Research Products

• [Journal Article] CpG-ODN 2006 and human parvovirus B19 genome consensus sequences selectively inhibit growth and development of erythroid progenitor cells. (2010)
  • Author(s): Guo YM, Ishii K, Hirokawa M, Tagawa H, Ohyagi H, Michishita Y, Ubukawa K, Yamashita J, Ohteki T, Onai N, Kawakami K, Xiao W, Sawada K
  • Journal Title: Blood. 115 Pages: 4569-4579
  • Peer Reviewed
• [Journal Article] Delayed addition of tumor necrosis factor (TNF) antagonists inhibits the generation of CD11c+ dendritic cells derived from CD34+ cells exposed to TNF-alpha. (2010)
  • Author(s): Guo YM, Hirokawa M, Takahashi N, Fujishima M, Fujishima N, Komatsuda A, Tagawa H, Ohyagi H, Michishita Y, Ubukawa K, Hebiguchi M, Xiao W, Sawada K
  • Journal Title: Int J Hematol. 91 Pages: 61-68
  • Peer Reviewed
• [Journal Article] Aberrant overexpression of microRNAs activate AKT signaling via down-regulation of tumor suppressors in natural killer-cell lymphoma/leukemia. (2009)
  • Author(s): Yamanaka Y, Tagawa H, Takahashi N, Watanabe A, Guo YM, Iwamoto K, Yamashita J, Saitoh H, Kameoka Y, Shimizu N, Ichinohasama R, Sawada K
  • Journal Title: Blood. 114 Pages: 3265-3275
  • Peer Reviewed
• [Journal Article] MicroRNA-17-92 down-regulates expression of distinct targets in different B-cell lymphoma subtypes. (2009)
  • Author(s): Inomata M, Tagawa H, Guo YM, Kameoka Y, Takahashi N, Sawada K
  • Journal Title: Blood. 113 Pages: 396-402
  • Peer Reviewed
• [Journal Article] Phagocytosis of co-developing neutrophil progenitors by dendritic cells in a culture of human CD34(+) cells with granulocyte colony-stimulating factor and tumor necrosis factor-alpha. (2008)
  • Author(s): Saito Y, Guo YM, Hirokawa M, Saito K, Komatsuda A, Takahashi N, Fujishima M, Fujishima N, Yamashita J, Sawada K
  • Journal Title: Int J Hematol. 88 Pages: 64-72
  • Peer Reviewed
• [Presentation] Establishment of a murine hemophagocytic syndrome model by administration of CpG and Mod1 ligand. (2010)
  • Author(s): Ohyagi H, Onai N, Guo YM, Takahashi N, Hirokawa M, Sawada K, Ohteki T
  • Organizer: 第72回日本血液学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 20100924-20100926
• [Presentation] CpG-ODN 2006 and parvovirus B19 genome consensus sequences selectively inhibit erythroid growth. (2010)
  • Author(s): Guo YM, Ishii K, Hirokawa M, Tagawa H, Ohyagi H, Michishita Y, Ubukawa K, Yamashita J, Ohteki T, Onai N, Kawakami K, Xiao W, Sawada K
  • Organizer: 第72回日本血液学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 20100924-20100926
• [Presentation] Establishment of a murine hemophagocytic syndrome model by administration of CpG and Nod1 ligand. (2010)
  • Author(s): Ohyagi H, Onai N, Guo YM, Takahashi N, Hirokawa M, Sawada K, Ohteki T
  • Organizer: 39^<th> The International Society of Experimental Hematology
  • Place of Presentation: Melbourne, Victoria, Australia
  • Year and Date: 2010-09-16
• [Presentation] CPG-ODN 2006 AND HUMAN PARVOVIRUS B19 GENOME CONSENSUS SEQUENCES SELECTIVELY INHIBIT GROWTH AND DEVELOPMENT OF ERYTHROID PROGENITOR CELLS. (2010)
  • Author(s): YM Guo, K Ishii, M Hirokawa, H Tagawa, H Ohyagi, Y Michishita, K Ubukawa, J Yamashita, T Ohteki, N Onai, K Kawakami, W Xiao, K Sawada
  • Organizer: 15^<th> Congress of the European Hematology Association Barcelona
  • Place of Presentation: Spain
  • Year and Date: 2010-06-11
• [Presentation] Prevention of Hemophagocytosis by TNF-α and IL-6 Blockade in TLR9- and Nod1- Ligand Induced Hemophagocytic Syndrome. (2009)
  • Author(s): Ohyagi H, Onai N, Guo Y-M, Takahashi N, Hirokawa M, Sawada K, Ohteki T
  • Organizer: Saturday, December 5, 2009, 51th Annual Scientific Meeting of The American Society of Hematology
  • Place of Presentation: New Orleans, Louisiana, USA
  • Year and Date: 20091205-20091208
• [Presentation] Aberrant over expressions of microRNAs activate AKT signaling in NK/T cell lymphoma/leukemia. (2009)
  • Author(s): Tagawa H., Ymanaka Y., Takahashi N., Y, M, Guo., Ichinohasama R., Shimizu N., Sawada K.
  • Organizer: 71回日本血液学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 20091023-20091025
• [Presentation] ABCG2 C.421C>A Is Associated with Higher Trough Imatinib Plasma Levels in Patients with Chronic Myeloid Leukemia. (2009)
  • Author(s): Takahashi N, Miura M, Scott SA, Sawada K
  • Organizer: 51th Annual Scientific Meeting of The American Society of Hematology
  • Place of Presentation: New Orleans, Louisiana, USA
  • Year and Date: 2009-12-06
• [Presentation] Aberrant Overexpressions of MicroRNA-21 and MicroRNA-155 Activate AKT Signaling Via Downregulation of Tumor Suppressors in NK-Cell Lymphoma/Leukemia. (2009)
  • Author(s): Tagawa H, Yamanaka Y, Watanabe A, Takahashi N, Sawada K
  • Organizer: 51th Annual Scientific Meeting of The American Society of Hematology
  • Place of Presentation: New Orleans, Louisiana, USA
  • Year and Date: 2009-12-05