Differentiation potential of Notch-1 positive human hematopoietic progenitors

2010 Fiscal Year Final Research Report

• Project/Area Number: 20591154
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hematology
• Research Institution: Kyushu University
• Principal Investigator: IWASAKI Hiromi Kyushu University, 大学病院, 講師 (20403925)
• Co-Investigator(Kenkyū-buntansha): AKASHI Koichi 九州大学, 医学研究院, 教授 (80380385)
• Project Period (FY): 2008 – 2010
• Keywords: 血液免疫学 / Notchシグナル / 樹状細胞

Research Abstract

We identified a novel human myeloid progenitor population that expressed Notch-1 at a high level. These Notch-1 positive progenitors preferentially differentiated into the monocyte/dendritic cell lineage in vitro. Notch-1 positive progenitors emerged in the culture of Notch-1 negative granulocyte/macrophage progenitors (GMPs), suggesting that they are more committed to the monocyte/dendritic cell lineage compared to GMPs. Indeed, they highly expressed monocyte/dendritic cell-affiliated genes including SpiB, FLT3, and PU.1. When Notch-1 positive progenitors were cultured in the presence of Notch-ligand (Delta-like 1), their progeny dendritic cells expressed lower levels of CD86 and HLA-DR compared to cells cultured without Delta-like 1, suggesting that Notch signals suppress the activation/maturation of dendritic cell lineage.

Research Products

• [Journal Article] 白血病幹細胞の成立機序と標的治療血液フロンティア (2010)
  • Author(s): 岩崎浩己、赤司浩一
  • Journal Title: 20(3) Pages: 149-158
• [Journal Article] Identification of the human eosinophil lineage-committed progenitor : Revision of phenotypic definition of the human common myeloid progenitor. (2009)
  • Author(s): Mori Y, Iwasaki H, Kohno K, Yoshimoto G, Kikushige Y, Okeda A, Uike N, Niiro H, Takenaka K, Nagafuji K, Miyamoto T, Harada M, Takatsu K, Akashi K
  • Journal Title: J Exp Med. 206 Pages: 183-93
  • Peer Reviewed
• [Journal Article] Origin of basophils and mast cells. (2009)
  • Author(s): 有信洋二郎, 岩崎浩己, 赤司浩一
  • Journal Title: Allergol Int. 58 Pages: 21-28
  • Peer Reviewed
• [Journal Article] 造血幹細胞から好酸球への分化機構血液・腫瘍科 (2009)
  • Author(s): 森康雄、岩崎浩己、赤司浩一
  • Journal Title: 58(2) Pages: 135-140
• [Journal Article] 好塩基球の発生・分化 (2009)
  • Author(s): 有信洋二郎、岩崎浩己、赤司浩一
  • Journal Title: アレルギー・免疫 6(3) Pages: 12-17
• [Journal Article] 白血病幹細胞の細胞生物学 (2009)
  • Author(s): 岩崎浩己、赤司浩一
  • Journal Title: 血液・腫瘍科 59(4) Pages: 467-475
• [Journal Article] Human flt3 is expressed at the hematopoietic stem cell and the granulocyte/macrophage progenitor stages to maintain cell survival. (2008)
  • Author(s): Kikushige Y, Yoshimoto G, Miyamoto T, Iino T, Mori Y, Iwasaki H, Niiro H, Takenaka K, Nagafuji K, Harada M, Ishikawa F, Akashi K
  • Journal Title: J Immunol. 180 Pages: 7358-7367
  • Peer Reviewed
• [Book] 好酸球、好塩基球、肥満細胞の分化経路実験医学 (2009)
  • Author(s): 岩崎浩己、赤司浩一
  • Total Pages: 56-62
  • Publisher: アレルギー疾患の免疫機構
• [Remarks] ホームページ等
  • URL: http://www.idenshi.co.jp/
• [Remarks]
  • URL: http://www.med.kyushu-u.ac.jp/intmedl/