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2010 Fiscal Year Final Research Report

Radiosensitization by cyclin-dependent kinase inhibitors and its molecular mechanism

Research Project

  • PDF
Project/Area Number 20591497
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Radiation science
Research InstitutionIbaraki Prefectural University of Health Science

Principal Investigator

KUBOTA Nobuo  Ibaraki Prefectural University of Health Science, 保健医療学部, 教授 (20046139)

Research Collaborator OKAYASU Ryuuichi  放射線医学総合研究所, 粒子線生物, グループリーダー
OHARA Maki  茨城県立医療大学, 保健医療学部, 嘱託助手
TANAKA Aya  茨城県立医療大学, 保健医療学部, 嘱託助手
Project Period (FY) 2008 – 2010
KeywordsCDK阻害剤 / イソチオシアネート / 放射線増感
Research Abstract

Isothiocyanates (ITCs) exhibit tumor prevention acivity. ITCs induce the upregulation of tumor suppressors CKI (CDK inhibitor) such as p21 and p27, and provide a protective effect to normal cells against cancer. Here, we report that Sulforaphane (SFN) and Benzyl isothiocyanate (BITC), enhance radiosensitivity in human tumor cells by repair inhibition of radiation-induced DNA double strand breaks through the impairment of nonhomologous end joining (NHEJ) and homologous recombination repair (HRR) pathways. SFN and BITC also synergisitically increase the radiation-induced apoptosis in human tumor cells. Our data suggest the potential use of SFN and BITC as an adjuvant to radiation therapy in the near future.

  • Research Products

    (16 results)

All 2011 2010 2009 2008 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (9 results) Remarks (1 results)

  • [Journal Article] Okayasu R, p53 independent radio-sensitization of human lymphoblastoid cell lines by Hsp90 inhibitor17-allylamino-17-demethoxygeldanamy cin.2010

    • Author(s)
      Fujii Y, Kato T, Kubota N, Fujimori A
    • Journal Title

      Oncol Rep 23(1)

      Pages: 199-203

    • Peer Reviewed
  • [Journal Article] Ascorbic acid gives different protective effects in human cells exposed to X-rays and heavy ions.2010

    • Author(s)
      Fujii Y, Kato T, Ueno A, Kubota N, Fujimori A, Okayasu R
    • Journal Title

      Mutat Res 699(1-2)

      Pages: 58-81

    • Peer Reviewed
  • [Journal Article] Chemopreventive agent sulforaphane enhances radiosensitivity in human tumor cells.2009

    • Author(s)
      Yu D, Sekine-Suzuki E, Xue L, Kubota N, Okayasu R
    • Journal Title

      Int J Cancer 125(5)

      Pages: 1205-1211

    • Peer Reviewed
  • [Journal Article] NBS1 regulates a novel apoptotic pathway through Bax activation.2008

    • Author(s)
      Iijima K, Muranaka C, Kobayashi J, SakamotoS, Komatsu K, Matsuura S, Kubota N, Tauchi H
    • Journal Title

      DNA Repair 7

      Pages: 1705-1716

    • Peer Reviewed
  • [Journal Article] Sulforaphane induces DNA double strand breaks predominantly repaired by homologous recombination pathway in human cancer cells.2008

    • Author(s)
      Sekine-Susuki E, Yu D, Kubota N, Okayasu R, Anzai K
    • Journal Title

      Biochem Biophys Res Com 377(2)

      Pages: 341-345

    • Peer Reviewed
  • [Journal Article] Scriptaid, a novel histone deacetylase inhibitor, enhances the response of human tumor cells to radiation.

    • Author(s)
      Kuribayashi T, Ohara M, Sora S, Kubota N
    • Journal Title

      Int J Mol Med 201,25(1)

      Pages: 25-29

    • Peer Reviewed
  • [Presentation] Hsp90阻害剤Celastrolのヒト腫瘍細胞の放射線増感2011

    • Author(s)
      瀬川達矢, 田中彩, 大西健, 窪田宜夫
    • Organizer
      第13回癌治療増感研究シンポジウム
    • Place of Presentation
      奈良
    • Year and Date
      20110200
  • [Presentation] 放射線増感作用を示す植物生理活性物質、特にフラボノイドの分子標的放射線増寒剤としての有用性2011

    • Author(s)
      大西健, 窪田宜夫, 竹森洋, 秦野修
    • Organizer
      第13回癌治療増感研究シンポジウム
    • Place of Presentation
      奈良
    • Year and Date
      20110200
  • [Presentation] 抗がん作用を持つフラボノイド類のスクリーニング2010

    • Author(s)
      大西健, 窪田宜夫, 秦野修, 竹森洋
    • Organizer
      第53回日本放射線影響学会
    • Place of Presentation
      京都
    • Year and Date
      20101100
  • [Presentation] Isothiocyanate類によるヒト腫瘍細胞の放射線増感2010

    • Author(s)
      窪田宜夫, 田中彩, 干冬, 岡安隆一
    • Organizer
      第12回癌治療増感研究シンポジウム
    • Place of Presentation
      奈良
    • Year and Date
      20100200
  • [Presentation] ヒト腫瘍細胞におけるBenzyl Isothiocyanateの放射線増感に関する研究2009

    • Author(s)
      窪田宜夫、大原麻希、木村慎一、関根恵美子、于冬、岡安隆一
    • Organizer
      第48回日本医学放射線学会生物部会
    • Place of Presentation
      富山
    • Year and Date
      20090600
  • [Presentation] ヒト腫瘍細胞でのイソチオシアネートによる放射線増感2009

    • Author(s)
      大原麻希、木村慎一、窪田宜夫、田内広
    • Organizer
      平成21年度日本原子力学会北関東支部若手研究者発表会
    • Place of Presentation
      水戸
    • Year and Date
      20090400
  • [Presentation] ヒト腫瘍細胞でのbenzyl isothiocyanateの放射線増感効果2008

    • Author(s)
      大原麻希、木村慎一、窪田宜夫
    • Organizer
      第51回日本放射線影響学会
    • Place of Presentation
      北九州
    • Year and Date
      20081100
  • [Presentation] スルフォラファンと重粒子線との併用における放射線増感による抗腫瘍効果の検討2008

    • Author(s)
      関根絵美子、于冬、二宮康晴、窪田宜夫、藤森亮、岡安隆一、安西和記
    • Organizer
      第51回日本放射線影響学会
    • Place of Presentation
      北九州
    • Year and Date
      20081100
  • [Presentation] DNA損傷修復経路によるスルフォラファンの放射線腫瘍増感作用2008

    • Author(s)
      于冬、関根絵美子、師連、藤森亮、窪田宜夫、岡安隆一
    • Organizer
      第51回日本放射線影響学会
    • Place of Presentation
      北九州
    • Year and Date
      20081100
  • [Remarks] ホームページ等

    • URL

      http://www.ipu.ac.jp/

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Published: 2012-01-26   Modified: 2016-04-21  

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