2010 Fiscal Year Final Research Report
The regenerative effect of autologous growth factors on nectotic bone.
| Project/Area Number |
20591735
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Yamagata University |
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Principal Investigator |
HASHIMOTO Junichi Yamagata University, 医学部, 講師 (10359565)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAGI Michiaki 山形大学, 医学部, 准教授 (40241707)
KIKUCHI Noriaki 山形大学, 医学部, 助教 (90361261)
WATANABE Tadayoshi 山形大学, 医学部, 助教 (90422170)
TABATA Yasuhiko 京都大学, 再生医科学研究所, 教授 (50211371)
NARITA Atsushi 山形大学, 医学部, 医員 (60400579)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 筋 / 神経病学 |
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| Research Abstract |
The aim of this study was to determine the appropriate concentration of platelet rich plasma (PRP) for a bone regeneration and evaluate the effect of PRP on MSCs. In in vitro study, we showed that PRP had a concentration-dependent effect on the cell proliferation. MSCs differentiated into osteoblasts with 125x10^4/μl and 62.5x10^4/ μl of PRP. In in vivo study, adequate bone regeneration was observed using 8x10^6 of MSCs and 2x10^8 of platelet. PRP could be candidate for safe and useful activator in clinical application.
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