2010 Fiscal Year Final Research Report
Experimental study for anterior cruciate ligament using cell sheet technology
| Project/Area Number |
20591747
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Tokai University |
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Principal Investigator |
MITANI Genya Tokai University, 医学部, 講師 (80307280)
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| Co-Investigator(Kenkyū-buntansha) |
SATOU Masato 東海大学, 医学部, 准教授 (10056335)
KUTUNA Toshiharu 東海大学, 医学部, 講師 (90328120)
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| Project Period (FY) |
2008 – 2010
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| Keywords | ACL / 細胞シート / 再生医療 / 前十字靭帯 / Tenomodulin |
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| Research Abstract |
Triple-layered ACL cell sheets and SCL-derived cell sheets were fabricated successfully without enzymatic digestion. Furthermore, 3-dimensional bioengineered ACL was fabricated by a combination of triple-layered ACL cell sheets and bioabsorbable mesh composite. Immunohistochemical examination and real-time PCR revealed that TnmD is a specific marker of the human ACL, and ACL sheets have a similar phenotype to the ACL. The superior expression of TnmD in synovial tissues, and synovium-derived cell sheets indicates that synovium is a potential cell source for ACL regeneration.
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